个人简历

讲授课程：
本科生：发育生物学
研究生：动物胚胎工程
教育经历：
1980.9-1984.7，四川畜牧兽医学院，本科学习
1980.9-1983.7，兽医大学，获得基础兽医学硕士
1992.9-1995.7，东北农业大学，获得生物工程博士学位
工作经历：
1987.9-1990.10， 兽医大学 助教
1990.11-1997.10, 兽医大学 讲师
1997.11-1998.10, 兽医大学 副教授
1998.1-2002.9, 美国密苏里大学 博士后
2002.10-2006.10,美国密苏里大学 研究助理教授
2007.10至今, 吉林大学 教授 博导
科研项目：
1．重点研发计划，神经疾病大动物模型的建立及干细胞治疗评价，2017.07-2021.12，2879万元。
2.科技部863计划，华南地区特种疾病相关胚胎干细胞库的建立 2006.1-2010.12， 226万
3. 科技部重大专项项目 “艾滋病和病毒性肝炎等重大传染病防治，人CD4和CCR5转基因兔作为HIV-1感染的动物模型 2009.1-2010.12， 752万
4. 国家973项目， 发育与生殖重要哺乳动物模型的建立2010.1-2015.12， 3000万
5. 转基因专项，广谱抗病毒DRACO转基因猪新品种培育， 2012.1-2016.12,2727万
6. 自然基金，高表达双链RNA激活凋亡寡聚体，2013.1-2016.12,85万
获奖情况：
1. 美国《科技新时代》杂志“医学技术最高奖获”得者 2002 年（
2. 两项研究成果分别在2002年和2006年被美国《发现》杂志评出的世界100大科技新闻中列前30位。
3. 我们的转基因绿猪照片和文字介绍被刊登在美国《国家地理杂志》 2005 年
4. 中国海外杰出青年基金获得者。
5. 国务院特殊津贴获得者

研究领域

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近期论文

1: Xie J, Ge W, Li N, Liu Q, Chen F, Yang X, Huang X, Ouyang Z, Zhang Q, Zhao Y, Liu Z, Gou S, Wu H, Lai C, Fan N, Jin Q, Shi H, Liang Y, Lan T, Quan L, Li X,Wang K, Lai L. Efficient base editing for multiple genes and loci in pigs using base editors. Nat Commun. 2019 Jun 28;10(1):2852. doi: 10.1038/s41467-019-10421-8. PubMed PMID: 31253764; PubMed Central PMCID:
PMC6599043.通讯作者
2: Liu Z, Chen M, Shan H, Chen S, Xu Y, Song Y, Zhang Q, Yuan H, Ouyang H, Li Z, Lai L. Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7). Cell Mol Life Sci. 2019 Oct;76(20):4155-4164. doi:10.1007/s00018-019-03110-8. Epub 2019 Apr 27. PubMed PMID: 31030226. 通讯作者
3: Sui T, Song Y, Liu Z, Chen M, Deng J, Xu Y, Lai L, Li Z. CRISPR-induced exon skipping is dependent on premature termination codon mutations. Genome Biol. 2018 Oct 17;19(1):164. doi: 10.1186/s13059-018-1532-z. PubMed PMID: 30333044; PubMed Central PMCID: PMC6193291. 通讯作者
4: Liu Z, Chen M, Chen S, Deng J, Song Y, Lai L, Li Z. Highly efficient RNA-guided base editing in rabbit. Nat Commun. 2018 Jul 13;9(1):2717. doi: 10.1038/s41467-018-05232-2. PubMed PMID: 30006570; PubMed Central PMCID:PMC6045575. 通讯作者
5: Wu H, Liu Q, Shi H, Xie J, Zhang Q, Ouyang Z, Li N, Yang Y, Liu Z, Zhao Y, Lai C, Ruan D, Peng J, Ge W, Chen F, Fan N, Jin Q, Liang Y, Lan T, Yang X, Wang X,Lei Z, Doevendans PA, Sluijter JPG, Wang K, Li X, Lai L. Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems. Cell Mol Life Sci. 2018 Oct;75(19):3593-3607. doi: 10.1007/s00018-018-2810-3. Epub 2018 Apr 10.PubMed PMID: 29637228. 通讯作者
6: Yan S, Tu Z, Liu Z, Fan N, Yang H, Yang S, Yang W, Zhao Y, Ouyang Z, Lai C,Yang H, Li L, Liu Q, Shi H, Xu G, Zhao H, Wei H, Pei Z, Li S, Lai L, Li XJ. A Huntingtin Knockin Pig Model Recapitulates Features of Selective Neurodegeneration in Huntington's Disease. Cell. 2018 May 3;173(4):989-1002.e13. doi: 10.1016/j.cell.2018.03.005. Epub 2018 Mar 29. PubMed PMID: 29606351; PubMed Central PMCID: PMC5935586. 通讯作者
7: Wang K, Jin Q, Ruan D, Yang Y, Liu Q, Wu H, Zhou Z, Ouyang Z, Liu Z, Zhao Y, Zhao B, Zhang Q, Peng J, Lai C, Fan N, Liang Y, Lan T, Li N, Wang X, Wang X, Fan Y, Doevendans PA, Sluijter JPG, Liu P, Li X, Lai L. Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing. Genome Res. 2017 Dec;27(12):2061-2071. doi: 10.1101/gr.222521.117. Epub 2017 Nov 16. PubMed PMID:
29146772; PubMed Central PMCID: PMC5741047. 通讯作者
8: Li L, Zhang Q, Yang H, Zou Q, Lai C, Jiang F, Zhao P, Luo Z, Yang J, Chen Q,Wang Y, Newsome PN, Frampton J, Maxwell PH, Li W, Chen S, Wang D, Siu TS, Tam S, Tse HF, Qin B, Bao X, Esteban MA, Lai L. Fumarylacetoacetate Hydrolase Knock-out Rabbit Model for Hereditary Tyrosinemia Type 1. J Biol Chem. 2017 Mar 17;292(11):4755-4763. doi: 10.1074/jbc.M116.764787. Epub 2017 Jan 4. PubMed PMID:28053091; PubMed Central PMCID: PMC5377789. 通讯作者
9: Sui T, Yuan L, Liu H, Chen M, Deng J, Wang Y, Li Z, Lai L. CRISPR/Cas9-mediated mutation of PHEX in rabbit recapitulates human X-linked hypophosphatemia (XLH). Hum Mol Genet. 2016 Jul 1;25(13):2661-2671. Epub 2016 Apr 28. PubMed PMID: 27126636. 通讯作者
10: Lv Q, Yuan L, Song Y, Sui T, Li Z, Lai L. D-repeat in the XIST gene is required for X chromosome inactivation. RNA Biol. 2016;13(2):172-6. doi: 10.1080/15476286.2015.1137420. PubMed PMID: 26786668; PubMed Central PMCID: PMC4829313. 通讯作者
11: Wang G, Yang H, Yan S, Wang CE, Liu X, Zhao B, Ouyang Z, Yin P, Liu Z, ZhaoY, Liu T, Fan N, Guo L, Li S, Li XJ, Lai L. Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain. Mol Neurodegener. 2015 Sep 3;10:42. doi: 10.1186/s13024-015-0036-5. PubMed PMID: 26334913; PubMed Central PMCID: PMC4557629. 通讯作者
12: Zhou X, Xin J, Fan N, Zou Q, Huang J, Ouyang Z, Zhao Y, Zhao B, Liu Z, Lai S, Yi X, Guo L, Esteban MA, Zeng Y, Yang H, Lai L. Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Cell Mol Life Sci. 2015 Mar;72(6):1175-84. doi: 10.1007/s00018-014-1744-7. Epub 2014 Oct 2. PubMed PMID: 25274063. 通讯作者
13: Huang J, Guo X, Fan N, Song J, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yan Q, Yi X, Schambach A, Frampton J, Esteban MA, Yang D, Yang H, Lai L. RAG1/2 knockout pigs with severe combined immunodeficiency. J Immunol. 2014 Aug 1;193(3):1496-503. doi: 10.4049/jimmunol.1400915. Epub 2014 Jun 27. PubMed PMID: 24973446. 通讯作者
14: Yang H, Wang G, Sun H, Shu R, Liu T, Wang CE, Liu Z, Zhao Y, Zhao B, Ouyang Z, Yang D, Huang J, Zhou Y, Li S, Jiang X, Xiao Z, Li XJ, Lai L. Species-dependent neuropathology in transgenic SOD1 pigs. Cell Res. 2014 Apr;24(4):464-81. doi: 10.1038/cr.2014.25. Epub 2014 Feb 28. PubMed PMID:
24577199; PubMed Central PMCID: PMC3975503. 通讯作者
15: Zou Q, Yan Q, Zhong J, Wang K, Sun H, Yi X, Lai L. Direct conversion of human fibroblasts into neuronal restricted progenitors. J Biol Chem. 2014 Feb 21;289(8):5250-60. doi: 10.1074/jbc.M113.516112. Epub 2014 Jan 2. PubMed PMID: 24385434; PubMed Central PMCID: PMC3931081. 通讯作者
16: Song J, Zhong J, Guo X, Chen Y, Zou Q, Huang J, Li X, Zhang Q, Jiang Z, Tang C, Yang H, Liu T, Li P, Pei D, Lai L. Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs. Cell Res. 2013 Aug;23(8):1059-62. doi: 10.1038/cr.2013.85. Epub 2013 Jul 9. PubMed PMID: 23835472; PubMed Central PMCID: PMC3731565. 通讯作者
17: Fan N, Chen J, Shang Z, Dou H, Ji G, Zou Q, Wu L, He L, Wang F, Liu K, Liu N,Han J, Zhou Q, Pan D, Yang D, Zhao B, Ouyang Z, Liu Z, Zhao Y, Lin L, Zhong C, Wang Q, Wang S, Xu Y, Luan J, Liang Y, Yang Z, Li J, Lu C, Vajta G, Li Z, Ouyang H, Wang H, Wang Y, Yang Y, Liu Z, Wei H, Luan Z, Esteban MA, Deng H, Yang H, Pei D, Li N, Pei G, Liu L, Du Y, Xiao L, Lai L. Piglets cloned from induced pluripotent stem cells. Cell Res. 2013 Jan;23(1):162-6. doi: 10.1038/cr.2012.176. Epub 2012 Dec 18. PubMed PMID: 23247628; PubMed Central PMCID: PMC3541650. 通讯作者
18: Yang D, Yang H, Li W, Zhao B, Ouyang Z, Liu Z, Zhao Y, Fan N, Song J, Tian J,Li F, Zhang J, Chang L, Pei D, Chen YE, Lai L. Generation of PPARγ mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning. Cell Res.2011 Jun;21(6):979-82. doi: 10.1038/cr.2011.70. Epub 2011 Apr 19. PubMed PMID: 21502977; PubMed Central PMCID: PMC3203707. 通讯作者
19: Lai L, Kang JX, Li R, Wang J, Witt WT, Yong HY, Hao Y, Wax DM, Murphy CN, Rieke A, Samuel M, Linville ML, Korte SW, Evans RW, Starzl TE, Prather RS, Dai Y. Generation of cloned transgenic pigs rich in omega-3 fatty acids. Nat Biotechnol. 2006 Apr;24(4):435-6. Epub 2006 Mar 26. PubMed PMID: 16565727; PubMed Central PMCID: PMC2976610.第一作者
20: Kolber-Simonds D, Lai L, Watt SR, Denaro M, Arn S, Augenstein ML, Betthauser J, Carter DB, Greenstein JL, Hao Y, Im GS, Liu Z, Mell GD, Murphy CN, Park KW,Rieke A, Ryan DJ, Sachs DH, Forsberg EJ, Prather RS, Hawley RJ. Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations. Proc Natl Acad Sci U S A.2004 May 11;101(19):7335-40. Epub 2004 May 3. PubMed PMID: 15123792; PubMed Central PMCID: PMC409919. 第一作者
21: Lai L, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science. 2002 Feb 8;295(5557):1089-92. Epub 2002 Jan 3. PubMed PMID: 11778012. 第一作者