个人简历

针对日趋严重的海岸带环境污染现状，开展了面向机制探索的海岸带典型污染物毒理效应及风险评价的研究工作。整合传统毒理学、系统生物学和计算毒理学手段，较为系统地研究了海岸带典型污染物(如重金属、多环芳烃和多溴联苯醚等)的急慢性毒性、内分泌干扰效应和遗传毒性，识别出多种表征污染物暴露的敏感生物标记物。相关结果先后以第一作者或通讯在Environmental Health Perspectives、Journal of Hazardous Materials、Chemosphere、Chemical Research in Toxicology、Marine Pollution Bulletin、Ecotoxicology and Environmental Safety以及《科学通报》等期刊上发表30余篇。研究结果为海岸带典型污染物的生态风险评价提供了毒理学数据，阐明了生物对海岸带环境污染物的响应机制，评价了海岸带污染物的生态风险性，为海岸带环境污染物的环境容量的计算提供基础数据与生物学依据。先后承担了国家自然科学基金重点（子课题）、面上项目、青年基金和国际科学基金（IFS）、参与了国家973计划课题、863重大项目课题等研究。至2019年12月，发表论文50多篇，授权发明专利3项，参与编写英文专著1部，中文专著2部。
研究内容
海洋生物对污染物胁迫（如重金属、阻燃剂和纳米材料等）的毒性效应及生态风险评估；海洋生物对环境污染物响应机制的计算毒理学研究；水生态系统健康快速监测及风险评估。
教育背景
2004-09-01_2010-06-30 大连理工大学 环境学院 博士
2000-09-01_2004-07-31 大连理工大学 学士
工作经历
2010-07-01_今 中国科学院烟台海岸带研究所 海岸带环境过程与生态修复重点实验室
研究项目
2017-01-01_2020-12-31 典型有机磷酸酯阻燃剂的跨膜机制及其与关键蛋白的相互作用
2016-01-01_2020-12-31 典型人工纳米颗粒在不同生态系统中的环境行为及食物链中的关键过程
2012-01-01_2014-12-31 典型有机磷酸酯阻燃剂对DNA功能基因的损伤机制和构效关系研究
2012-09-01_2015-12-31 Binding Interactions Between Anthraquinone Derivatives and DNA: An Integrated Experimental Determination and Molecular Simulation Approach
2011-01-01_2013-12-31 多环芳烃对DNA损伤机制的计算模拟与验证
专利
[1] 王晓晴,李斐,刘佳琳,等. 评估和预测有机磷酸酯阻燃剂对模拟生物膜损伤的方法. 评估和预测有机磷酸酯阻燃剂对模拟生物膜损伤的方法. 2018-12-21.
[2] 李斐,曹璐璐,吴惠丰,等. 一种预测生物标志物p53与有机磷酸酯阻燃剂相互作用亲和力的方法. 2014-04-18.
[3] 李斐,吴惠丰,曹璐璐,等. 预测海岸带持久性有机污染物消除速率的方法. 2014-01-07.

研究领域

环境科学 生态毒理学、计算毒理学""

近期论文

[1] Ji, Chenglong.,Lu, Zhen.,Xu, Lanlan.,Li, Fei.,Cong, Ming.,...&Wu, Huifeng.(2020).Global responses to tris(1-chloro-2-propyl)phosphate (TCPP) in rockfish Sebastes schlegeli using integrated proteomic and metabolomic approach.SCIENCE OF THE TOTAL ENVIRONMENT,724.
[2] 孟祥敬,李斐,王晓晴,等. 石墨烯联合磷酸三苯酯(TPP)胁迫紫贻贝(Mytilus galloprovincialis)的生理生化响应[J]. 科学通报,2020,65(16):1599-1609.
[3] Sun, Tao,Wu, Huifeng,Cong, Ming,Zhan, Junfei,&Li, Fei.(2020).Meta-analytic evidence for the anti-aging effect of hormesis on Caenorhabditis elegans.AGING-US,12(3),2723-2746.
[4] Meng, Xiangjing,Li, Fei,Wang, Xiaoqing,Liu, Jialin,Ji, Chenglong,&Wu, Huifeng.(2020).Toxicological effects of graphene on mussel Mytilus galloprovincialis hemocytes after individual and combined exposure with triphenyl phosphate.MARINE POLLUTION BULLETIN,151,110838.
[5] Lu, Z.,Wang, S.,Ji, CL.,Li, F.,Cong, M.,...&Wu, HF.(2020).iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium.ENVIRONMENTAL POLLUTION,257,113591.
[6] Xu, LL.,Lu, Z.,Ji, CL.,Cong, M.,Li, F.,...&Wu, HF.(2019).Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach.ENVIRONMENTAL POLLUTION,255(2),113333.
[7] Meng, XJ,Li, F,Wang, XQ,Liu, JL,Ji, CL,&Wu, HF.(2019).Combinatorial immune and stress response, cytoskeleton and signal transduction effects of graphene and triphenyl phosphate (TPP) in mussel Mytilus galloprovincialis.JOURNAL OF HAZARDOUS MATERIALS,378,UNSP 120778.
[8] Ji, CL.,Lu, Z.,Xu, LL.,Li, F.,Cong, M.,...&Wu, HF.(2019).Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics.ENVIRONMENTAL POLLUTION,251,802-810.
[9] Wang, XQ,Meng, XJ,Li, F,Ding, JW,Ji, CL,&Wu, HF.(2019).The critical factors affecting typical organophosphate flame retardants to mimetic biomembrane: An integrated in vitro and in silico study.CHEMOSPHERE,226,159-165.
[10] Zhu, N.,Yang, YY.,Xu, H.,Wang, Q.,Wei, YY.,...&Fang, Y.(2019).Bioaccumulation of decabromodiphenyl ether affects the antioxidant system in the clam Mactra veneriformis.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,68,19-26.
[11] 王晓晴,刘佳琳,李斐,等. 磷酸三苯酯(TPP)和磷酸三(2-氯异丙基)酯(TCPP)诱导人胚肾细胞HEK293凋亡的机制研究[J]. 生态毒理学报,2019,14(5):123-132.
[12] 李斐,王晓晴,刘佳琳,等. 溴系阻燃剂毒理效应的计算模拟预测与环境风险评估进展[J]. 生态毒理学报,2019,14(4):2-13.
[13] Xu, Lina.,Dai, Yanhui.,Wang, Zhenyu.,Zhao, Jian.,Li, Fei.,...&Xing, Baoshan.(2018).Graphene quantum dots in alveolar macrophage: uptake-exocytosis, accumulation in nuclei, nuclear responses and DNA cleavage.PARTICLE AND FIBRE TOXICOLOGY,15,45.
[14] Yang, Dinglong.,Zhang, Qianqian.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2018).A defensin-like antimicrobial peptide from the manila clam Ruditapes philippinarum: Investigation of the antibacterial activities and mode of action.FISH & SHELLFISH IMMUNOLOGY,80,274-280.
[15] Wu, Huifeng.,Zhong, Mingyu.,Lu, Zhen.,Shan, Xiujuan.,Li, Fei.,...&Cong, Ming.(2018).Biological effects of tris (1-chloro-2-propyl) phosphate (TCPP) on immunity in mussel Mytilus galloprovincialis.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,61,102-106.
[16] Cao, Ruiwen.,Wang, Qing.,Yang, Dinglong.,Liu, Yongliang.,Ran, Wen.,...&Zhao, Jianmin.(2018).CO2-induced ocean acidification impairs the immune function of the Pacific oyster against Vibrio splendidus challenge: An integrated study from a cellular and proteomic perspective.SCIENCE OF THE TOTAL ENVIRONMENT,625,1574-1583.
[17] Li, Fei.(2018).Interaction between organic phosphate ester and p53: An integrated experimental and in silico approach.ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY,255.
[18] Zhong, Mingyu.,Wu, Huifeng.,Mi, Wenying.,Li, Fei.,Ji, Chenglong.,...&Tang, Jianhui.(2018).Occurrences and distribution characteristics of organophosphate ester flame retardants and plasticizers in the sediments of the Bohai and Yellow Seas, China.SCIENCE OF THE TOTAL ENVIRONMENT,615(0),1305-1311.
[19] 李斐,丁家旺,吴惠丰,等. 电化学方法研究石墨烯和磷酸三苯酯(TPP)与模拟生物膜间的相互作用[J]. 科学通报,2017,62(36):4285-4293.
[20] Li, Fei.,Wang, Li.,Ji, Chenglong.,Wu, Huifeng.,Zhao, Jianmin.,...&Wu, Huifeng.(2017).Toxicological effects of tris(2-chloropropyl) phosphate in human hepatic cells.CHEMOSPHERE,187,88-96.
[21] Xie, Jia.,Yang, Dinglong.,Sun, Xiyan.,Cao, Ruiwen.,Chen, Lizhu.,...&Zhao, Jianmin.(2017).Individual and Combined Toxicities of Benzo[a]pyrene and 2,2',4,4'-Tetrabromodiphenyl Ether on Early Life Stages of the Pacific Oyster, Crassostrea gigas.BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,99(5),582-588.
[22] Yu, Qian.,Yang, Dinglong.,Wang, Qing.,Zhang, Yingying.,Cong, Ming.,...&Zhao, Jianmin.(2017).Molecular characterization, expression and functional analysis of two Kazal-type serine protease inhibitors from Venerupis philippinarum.FISH & SHELLFISH IMMUNOLOGY,70,156-163.
[23] Xie, J.,Yang, DL.,Sun, XY.,Cao, RW.,Chen, LZ.,...&Zhao, JM.(2017).Individual and Combined Toxicities of Benzo[a]pyrene and 2,2',4,4'-Tetrabromodiphenyl Ether on Early Life Stages of the Pacific Oyster, Crassostrea gigas.BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,99(5),582-588.
[24] 刘佳琳,徐兰兰,路珍,等. 基于核磁共振的褐牙鲆幼鱼组织代谢组学分析[J]. 海洋通报,2017,36(5):538-546.
[25] Zhong, Mingyu.,Tang, Jianhui.,Mi, Lijie.,Li, Fei.,Wang, Runmei.,...&Tang, Jianhui.(2017).Occurrence and spatial distribution of organophosphorus flame retardants and plasticizers in the Bohai and Yellow Seas, China.MARINE POLLUTION BULLETIN,121(1-2),331-338.
[26] Yang, Dinglong.,Wang, Qing.,Cao, Ruiwen.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2017).Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,73,109-118.
[27] 吴惠丰,张明兴,李斐,等. 石墨烯与抑癌基因p53 DNA片段相互作用的分子模拟与光谱学验证[J]. 生态毒理学报,2017,12(3):243-250.
[28] Yang, Dinglong.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,Cao, Ruiwen.,...&Zhao, Jianmin.(2017).Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,65,17-24.
[29] 谢嘉,滕佳,刘永亮,等. Cd~(2+)和Pb~(2+)单一与复合污染对脊尾白虾的急性毒性效应研究[J]. 海洋科学,2017,41(5):27-33.
[30] Xie, J..,Yang, D..,Sun, X..,Cao, R..,Chen, L..,...&Zhao, J..(2017).Combined toxicity of cadmium and lead on early life stages of the Pacific oyster, Crassostrea gigas.ISJ-INVERTEBRATE SURVIVAL JOURNAL,14,210-220.
[31] Xie, Jia.,Zhao, Yun.,Wang, Qing.,Wu, Huifeng.,Teng, Jia.,...&Zhao, JM .(2016).An integrative biomarker approach to assess the environmental stress in the north coast of Shandong Peninsula using native oysters, Crassostrea gigas (vol 112, pg 318, 2016).MARINE POLLUTION BULLETIN,113(1-2),600-600.
[32] Yang, Xianhai.,Liu, Huihui.,Liu, Jining.,Li, Fei.,Li, Xuehua.,...&jwchen@dlut.edu.cn.(2016).Rational Selection of the 3D Structure of Biomacromolecules for Molecular Docking Studies on the Mechanism of Endocrine Disruptor Action.CHEMICAL RESEARCH IN TOXICOLOGY,29(9),1565-1570.
[33] Ji, CL.,Yu, DL.,Wang, Q.,Li, F.,Zhao, JM.,...&Wu, HF .(2016).Impact of metal pollution on shrimp Crangon affinis by NMR-based metabolomics.MARINE POLLUTION BULLETIN,106(1-2),372-376.
[34] Yang, HB,Li, F,Ji, NY,Li, F ,Ji, NY ,&nyji@yic.ac.cn.(2016).Alkaloids from an algicolous strain of Talaromyces sp..CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,34(2),367-371.
[35] Hu, XP.,Pan, DW.,Lin, MY.,Han, HT.,Li, F.,...&Pan, DW .(2016).Graphene oxide-assisted synthesis of bismuth nanosheets for catalytic stripping voltammetric determination of iron in coastal waters.MICROCHIMICA ACTA,183(2),855-861.
[36] Ji, CL.,Li, F.,Wang, Q.,Zhao, JM.,Sun, ZD.,...&Wu, HF .(2016).An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis to tetrabromobisphenol A (TBBPA).CHEMOSPHERE,144,527-539.
[37] 钟鸣宇　李斐　唐建辉　吴惠丰　赵建民,李斐. 有机磷酸酯在环境/ 生物相中的分布、分析方法及毒理效应[J]. 海岸科学,2016,第3卷(第1期):59-70.
[38] Lin, MY,Pan, DW,Hu, XP,Han, HT,Li, F,&Pan, DW .(2015).Titanium carbide nanoparticles/ion-exchange polymer-based sensor for catalytic stripping determination of trace iron in coastal waters.SENSORS AND ACTUATORS B-CHEMICAL,219,164-170.
[39] Han, HT,Pan, DW,Lin, MY,Hu, XP,Li, F,&Han, HT .(2015).In-situ Synthesis of Graphitic Carbon Nitride/Gold Nanoparticle Nanocomposites for Electrocatalytic Reduction of Iron(III).INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE,10(11),9006-9014.
[40] Li, Fei.,Pan, Dawei.,Lin, Mingyue.,Han, Haitao.,Hu, Xueping.,...&qikang@sdu.edu.cn.(2015).Electrochemical determination of iron in coastal waters based on ionic liquid-reduced graphene oxide supported gold nanodendrites.ELECTROCHIMICA ACTA,176,548-554.
[41] 杨先海,陈景文,李斐. 化学品甲状腺干扰效应的计算毒理学研究进展[J]. 科学通报,2015,60(19):1761-1771.
[42] 吴惠丰,曹璐璐,李斐,等. 典型持久性有机污染物与抑癌基因相互作用的分子模拟与验证[J]. 科学通报,2015,60(19):1804-1809.
[43] Ding, Jiawang,Gu, Yue,Li, Fei,Zhang, Hongxia,&Qin, Wei.(2015).DNA Nanostructure-Based Magnetic Beads for Potentiometric Aptasensing.ANALYTICAL CHEMISTRY,87(13),6465-6469.
[44] Li, Fei,Zhang, Qing,Pan, Dawei,Lin, Mingyue,Kang, Qi,&Kang, Q .(2015).Electrochemical determination of tryptophan at room-temperature ionic liquid-titanium carbide nanoparticle gel modified electrode.IONICS,21(6),1711-1718.
[45] Wu, Huifeng,Cao, Lulu,Li, Fei,Lian, Peiwen,Zhao, Jianmin,&Li, F .(2015).Multiple biomarkers of the cytotoxicity induced by BDE-47 in human embryonic kidney cells.CHEMOSPHERE,126,32-39.
[46] Li Fei,Wu Huifeng,Wang Qing,Li Xuehua,Zhao Jianmin,&Wu, HF .(2015).Glutathione S-transferase (GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(3),705-713.
[47] Ji, Chenglong,Cao, Lulu,Li, Fei,&Li, F .(2015).Toxicological evaluation of two pedigrees of clam Ruditapes philippinarum as bioindicators of heavy metal contaminants using metabolomics.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,39(2),545-554.
[48] Wei, Lei.,Wang, Qing.,Ning, Xuanxuan.,Mu, Changkao.,Wang, Chunlin.,...&jmzhao@yic.ac.cn.(2015).Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,13,16-23.
[49] Li, Fei.,Cao, Lulu.,Li, Xuehua.,Li, Na.,Wang, Zijian.,...&Wu, HF .(2015).Affinities of organophosphate flame retardants to tumor suppressor gene p53: An integrated in vitro and in silico study.TOXICOLOGY LETTERS,232(2),533-541.
[50] 于德良 李斐 吴惠丰 赵建民,李斐. 海岸带污染物联合毒性的预测及评价[J]. 海岸科学,2015,第2卷(第1期):27-36.
[51] Hu, XP,Pan, DW,Lin, MY,Han, HT,Li, F,&Hu, XP .(2015).One-Step Electrochemical Deposition of Reduced Graphene Oxide-Bismuth Nanocomposites for Determination of Lead.ECS ELECTROCHEMISTRY LETTERS,4(9),H43-H45.
[52] Lin, MY,Pan, DW,Hu, XP,Li, F,Han, HT,&Pan, DW .(2015).A tin-bismuth alloy electrode for the cathodic stripping voltammetric determination of iron in coastal waters.ANALYTICAL METHODS,7(12),5169-5174.
[53] Li, Fei.,Yang, Xianhai.,Li, Xuehua.,Li, Renmin.,Zhao, Jianmin.,...&hfwu@yic.ac.cn.(2014).Determination and Prediction of the Binding Interaction between Organophosphate Flame Retardants and p53.CHEMICAL RESEARCH IN TOXICOLOGY,27(11),1918-1925.
[54] Li, Fei.,Li, Renmin.,Yang, Xianhai.,You, Liping.,Zhao, Jianmin.,...&hfwu@yic.ac.cn.(2014).The interaction between organic phosphate ester and p53: An integrated experimental and in silico approach.MARINE POLLUTION BULLETIN,85(2),516-521.
[55] Liu, Xiaoli.,Ji, Chenglong.,Zhao, Jianmin.,Wang, Qing.,Li, Fei.,...&Liu, XL .(2014).Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge.FISH & SHELLFISH IMMUNOLOGY,39(2),372-377.
[56] 林晓玲,王清,袁泽轶,等. 菲律宾蛤仔(Ruditapes philippinarum)金属硫蛋白基因克隆及Cd~(2+)胁迫下的表达分析[J]. 海洋与湖沼,2014,45(5):1006-1012.
[57] Li, Fei,Wu, Huifeng,Li, Lianzhen,Li, Xuehua,Zhao, Jianmin,&Peijnenburg, Willie J. G. M..(2012).Docking and QSAR study on the binding interactions between polycyclic aromatic hydrocarbons and estrogen receptor.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,80,273-279.
[58] Li, Fei.,Li, Xuehua.,Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,...&Wu, Huifeng.(2011).Docking and 3D-QSAR studies on the Ah receptor binding affinities of polychlorinated biphenyls (PCBs), dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs).ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,32(3),478-485.
[59] Li, Fei.,Li, Xuehua.,Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,...&Wu, Huifeng.(2011).Noncovalent interactions between hydroxylated polycyclic aromatic hydrocarbon and DNA: Molecular docking and QSAR study.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,32(3),373-381.
[60] Zhang, Linbao.,Liu, Xiaoli.,You, Liping.,Zhou, Di.,Wang, Qing.,...&Wu, Huifeng.(2011).Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance (H-1 NMR) based metabolomics.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,32(2),218-225.
[61] Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Wu, Huifeng.,Zhao, Jianmin.,...&Yu, Junbao.(2011).Metabolomic Study on the Halophyte Suaeda salsa in the Yellow River Delta.CLEAN-SOIL AIR WATER,39(8),720-727.
[62] Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Yu, Junbao.,Cong, Ming.,...&Wu, Huifeng.(2011).Assessment of Clam Ruditapes philippinarum as Heavy Metal Bioindicators Using NMR-Based Metabolomics.CLEAN-SOIL AIR WATER,39(8),759-766.
[63] Qiao, Xianliang,Carmosini, Nadia,Li, Fei,&Lee, Linda S..(2011).Probing the Primary Mechanisms Affecting the Environmental Distribution of Estrogen and Androgen Isomers.ENVIRONMENTAL SCIENCE & TECHNOLOGY,45(9),3989-3995.
[64] Li, F..,Liu, X..,Zhang, L..,You, L..,Wu, H..,...&Yu, J..(2011).QSAR studies on the depuration rates of polycyclic aromatic hydrocarbons, polybrominated diphenyl ethers and polychlorinated biphenyls in mussels (Elliptio complanata).SAR AND QSAR IN ENVIRONMENTAL RESEARCH,22(5-6),561-573.
[65] Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Yu, Junbao.,Zhao, Jianmin.,...&Wu, Huifeng.(2011).Differential toxicological effects induced by mercury in gills from three pedigrees of Manila clam Ruditapes philippinarum by NMR-based metabolomics.ECOTOXICOLOGY,20(1),177-186.