孙方臻
近期热点
资料介绍
个人简历
该实验室主要研究方向是受精过程中卵激活和精核重建的机理研究。受精是生殖与发育的一个必不可少的过程。我们的目的是在分子和细胞水平上搞清楚受精的两个重要事件,即由精子引起的卵激活和由卵子因子诱导的精核重建。我们的研究聚焦在鉴定决定这些事件的父源和母源分子,搞清楚其作用机理。这些研究可以提高核移植胚的胚胎发育率,并应用于动物克隆或人类治疗性克隆研究。目前,我们的主要聚焦点是搞清楚精子如何通过引起细胞内钙离子浓度瞬间升高(一个到目前为止在所研究的动物种类中均观察到的现象),启动储存在卵子内的胚胎发育程序。这个钙信号过程在卵激活和早期胚胎发育中起关键作用。我们的研究证据显示在哺乳动物卵子内钙振荡的有序进行主要依赖于两个必要因子:一个是触发钙从细胞内钙库释放的来自精子内的蛋白因子;而另一个是决定振荡过程是否可以持续的母源性装置。我们已证明来源于精子的蛋白因子的活性在进化上是高度保守的;从植物精子中提取的提取物显微注射到小鼠卵内可以诱导小鼠卵发生钙振荡。我们正准备纯化这个精子蛋白因子并搞清楚这个因子如何通过IP3受体使钙从细胞内钙库中释放的。我们首次报道了精子因子诱导的钙振荡是受哺乳动物卵子内一个母源性装置调控的,该装置的功能是一次性的。现在我们要研究的是这个母源性装置的性质和特性。此外,我们要确定精子蛋白因子用于触发钙从细胞内钙库释放的特异信号传导通路。近期论文
Gui-RongBai,Ling-HaiYang,Xiu-YingHuang,Fang-ZhenSun.Inositol1,4,5-trisphosphatereceptortype1phosphorylationandregulationbyextracellularsignal-regulatedkinase.Biochem.Biophys.Res.Commun.,348:1319-1327.2006Ling-HaiYang,Gui-RongBai,Xiu-YingHuang,Fang-ZhenSun.ERKbinds,phosphorylatesInsP3type1receptorandregulatesintracellularcalciumdynamicsinDT40cells.Biochem.Biophys.Res.Commun.,349:1339-1344.2006.周光炎,孙方臻主编《异种移植》,上海科学技术出版社,2006.DiZhang,LeiPan,Ling-HaiYang,Xiao-KeHe,Xiu-YingHuangandFang-ZhenSun.Sr2+promotescalciumoscillationsinmousemeioticoocytesandmitoticembryosthroughInsP3receptorsandrequireactivationofPLCandasynergisticactionofInsP3.HumanReproduction,20(11):3053-3061,2005.LongboCui,XiuyingHuangandFang-ZhenSun.Transferofgerminalvesicletoooplasmofyoungmicecouldnotrescueageing-associatedchromosomemisalignmentinmeiosisofoocytesfromagedmice.HumanReproduction,20(6):1624-31.2005.LongboCui,XiuyingHuangandFang-ZhenSun.NucleocytoplasmicRatioofFullyGrowngerminalvesicleOocytesIsEssentialforMouseMeioticChromosomeSegregationandAlignment,SpindleShapeandEarlyEmbryonicDevelopment.HumanReproduction,20(10):2946-2953,2005.Qi-CaiLiu,Tian-eChen,Xiu-YingHuangandFang-ZhenSun.Mammalianfreeze-driedspermcanmaintaintheircalciumoscillation-inducingabilitywhenmicroinjectedintomouseeggs.Biochem.Biophys.ResearchCommun.,328:824-830,2005Guang-JianJiang,LeiPan,Xiu-YingHuang,MeiHan,Jin-KunWen,Fang-ZhenSun.ExpressionofHSGisessentialformouseblastocystformation.Biochem.Biophys.ResearchCommun.,335(2):351-355.,2005.Sun,F.Z.,Bradshaw,J.P.,Calli,C.,&Moor,R.M.,Changesinintracellularcalciumconcentration([Ca2+]i)inbovineoocytesfollowingspermpenetration.J.Reprod.Fertil.,101:713-719.1994.Sun,F.Z.andMoor,R.M.Nucleartransplantationinmammalianeggsandembryos.CurrenttopicsinDevelopmentalBiology32:147-176.1995。Deng,M.Q.,andSun,F.Z.Thefertilization-inducedCa2+oscillationinmouseoocytesiscytoplasmicmaturationdependent.CellResearch6:167-175.1996。Zalewski,P.D.,Jian,X.,Soon,L.L.L.,Breed,W.G.,Seamark,R.F.,Lincoln,S.F.,Ward,A.D.,andSun,F.Z.Changesindistributionoflabilezincinmousespermatozoaduringmaturationintheepididymisassessedbythefluorophorezinquin.Reprod.Fertil.Dev.8:1097-1105,1996。曾一青,孙方臻.利用基因诱捕识别哺乳动物发育调控基因.生物化学与生物物理进展.23(3):194-200,1996.HeWei,GaoJian-gang,LiuXiaoandSunFang-zhen.Establishmentofembryonicstemcelllinesderivedfromoutbredmouseembryosandproductionofchimeras.Dev.Reprod.Biol.,5(2):21-25,1996.邓满齐,辛俭,黄秀英,孙方臻小鼠卵母细胞成熟和老化的不同阶段受精诱导的胞质Ca2+波动特点.科学通报.42(3):317-320.1997。Deng,M.Q.,Huang,X.Y.,Tang,T.S.,andSun,F.Z.,Spontaneousandfertilization-inducedCa2+oscillationinmouseimmatureGVstageoocytes.Biol.Reprod.56(suppl.1)588,1997.DengManqi,HuangXiuying,TangTieshan&SunFangzhen.Spontaneousandfertilization-inducedCa2+oscillationinmouseimmatureGVstageoocytes.Biol.Reprod.,58(3):807-813,1998.DengManqi,HuangXiuying,&SunFangzhen.Intracellularcalciumresponsestofertilizationinmouseeggsmodifiedduringposrovulatoryagingprocess.BiologyofReproductions,Suppl.,58:279,1998.黄秀英,劳为德,郑瑞珍等:《发育生物学》,高等教育出版社,1998。LiShuhong,GuoShaodong,&SunFangzhen.ANovelgeneexpressedduringearlyembryogenesisofzebrafishidentifiedbymRNAdifferentialdisplay.ChineseScienceBulletin,43(11):936-940,1998.LiShuhong,GuoShaodong,&SunFangzhen.ExpressionpatternofaBeta-IItubulingeneinzebrafishembryosidentifiedbywholemountinsituhybridization.ChineseScienceBulletin,43(17):759-763,1998.白成,乔殿华,孙方臻。表达人膜辅助因子蛋白基因的猪内皮细胞可抑制人血清补体的细胞毒作用。中华微生物学和免疫学杂志19(2):133-136,1999。Dong,J.B.,Tang,T.S.andSun,F.Z.Xenopusandchickenspermcontainacytosolicsolublefactorwhichcantriggercalciumoscillationsinmouseeggs.Biochem.Biophys.ResearchCommun.,268:947-951,2000.Tang,T.S.,Dong,J.B.,Huang,X.Y.andSun,F.Z.Ca2+oscillationsinducedbyacytosolicspermproteinfactoraremediatedbyamaternalmachinerythatfunctionsonlyonceinmammalianeggs.Development,127:1141-1151,2000.严进,何成,路长林,王成海,鲍璿,唐铁山,黄秀英,孙方臻。CNTF信号转导途径上游的新支路。科学通报,45(18):1978-1981,2000。Li,S.T.,Huang,XY.,&Sun,F.Z.Floweringplantspermcontainsacytosolicsolubleproteinfactorwhichcantriggercalciumoscillationsinmouseeggs.Biochem.Biophys.ResearchCommun.,287(1):56-59,2001。向国胜,孙方臻。转录因子NF-kB/IkB。细胞生物学杂志,23(2):71-77,2001。 相关热点
最新收录
- 千奈美(ちなみん) 06-25
- 皆川琉衣(皆川るい) 06-25
- 凪光(凪ひかる) 06-25
- 织本濑里乃(織本せりの 06-25
- 冈本莉里 岡本莉里 (おか 06-25
- 凯蒂·佩里(水果姐) 06-25
- 吉高宁宁(吉高寧々 Nene 06-25
- 水咲优美(水咲優美) 06-25
- 三月光(三月ひかる) 06-21
- 澪川遥(澪川はるか) 06-21