个人简历

1. 2008.09~至今 浙江理工大学 生命科学学院 副研究员
2. 2006.04~2008.08 浙江理工大学 生命科学学院 助理研究员
3. 1999.09~2006.03 浙江大学 生物化学与分子生物学专业 博士
4.1995.09~1999.06 华中农业大学 畜牧兽医学院兽医专业 本科

研究领域

1. 家蚕生物反应器
2. 杆状病毒分子生物学

近期论文

1. 费伟强, 陈琴, 陈倩, 梅文枫, 边腾飞, 吕正兵, 于威, 陈健*, 张耀洲, 吴祥甫. 复制缺陷型BmNPV载体的构建及初步应用. 蚕业科学, 2013, 39（3）：0501-0508.
2. 陈和, 陈琴, 梅文枫, 钱月忠, 聂作明, 于威, 陈健*, 张耀洲. 一种红色荧光蛋白基因稳定转化的家蚕细胞株构建及应用. 蚕业科学, 2013, 39（3）：0494-0500.
3. 陈琴, 杨丹, 聂作明, 陈健, 张耀洲. 家蚕脂蛋白基因BmLp-c21的表达及亚细胞定位. 蚕业科学, 2012, 39（3）：0434-0441.
4. Liu Y, Chen Y, Chen J, Zhang W, Sheng Q, Chen J, Yu W, Nie Z, Zhang Y, Wu W, Wang L, Indran IR, Li J, Qian L, Lv Z. A Shark Liver Gene-Derived Active Peptide Expressed in the Silkworm, Bombyx mori: Preliminary Studies for Oral Administration of the Recombinant Protein. Mar Drugs. 2013, 11(5):1492-1505.
5. Yu W, Du CY, Quan YP, Nie ZM, Chen J, Lv ZB, Zhang YZ. Characterization of late gene expression factor LEF-10 from Bombyx mori nucleopolyhedrovirus. Virus Res. 2013, 175(1): 45-51.
6. Zhou F, Gao Z, Lv Z, Chen J, Hong Y, Yu W, Wang D, Jiang C, Wu X, Zhang Y, Nie Z. Construction of the ie1-Bacmid Expression System and Its Use to Express EGFP and BmAGO2 in BmN Cells. Appl Biochem Biotechnol. 2013 Apr; 169(8): 2237-2247.
7. Yang L, Lu X, Liu Y, Lv Z, Chen J, Yu W, Zhang Y, Nie Z. Expression analysis of miRNAs in BmN cells. Gene. 2012 Sep 1; 505(2): 240-245.
8. Yu W, Li J, Wang M, Quan Y, Chen J, Nie Z, Lv Z, Zhang Y. The screening and functional study of proteins binding with the BmNPV polyhedrin promoter. Virol J. 2012 May 6; 9:90.
9. Quan Y, Liu G, Yu W, Nie Z, Chen J, Lv Z, Zhang Y. Expression, Purification, and Characterization of Ras Protein (BmRas1) from Bombyx mori. Comp Funct Genomics. 2012; 2012:747539.
10. Lv Z, Zhang X, Liu L, Chen J, Nie Z, Sheng Q, Zhang W, Jiang C, Yu W, Wang D, Wu X, Zhang S, Li J, Zhang Y. Characterization of a gene encoding prohibitin in silkworm, Bombyx mori. Gene. 2012 Jul 10; 502(2):118-124.
11. Chen J, Gai Q, Lv Z, Chen J, Nie Z, Wu X, Zhang Y. All-trans retinoic acid affects subcellular localization of a novel BmNIF3l protein: functional deduce and tissue distribution of NIF3l gene from silkworm (Bombyx mori). Arch Insect Biochem Physiol. 2010 Aug; 74(4): 217-231.
12. Lan H, Nie Z, Liu Y, Lv Z, Liu Y, Quan Y, Chen J, Zhen Q, Chen Q, Wang D, Sheng Q, Yu W, Chen J, Wu X, Zhang Y. In vivo bioassay of recombinant human growth hormone synthesized in B. mori pupae. J Biomed Biotechnol. 2010; 2010:306462.
13. Zeng F, Xie H, Nie Z, Chen J, Lv Z, Chen J, Wang D, Liu L, Yu W, Sheng Q, Wu X, Zhang Y. Characterization of the gene BmEm4, a homologue of Drosophila E(spl)m4, from the silkworm, Bombyx mori. Comp Funct Genomics. 2009: 947490.
14. Shen Y, Zhang T, Chen J, Lv Z, Chen J, Wang D, Nie Z, He P, Wang J, Zheng Q, Sheng Q, Wu X, Zhang Y. Molecular characterization and tissue localization of an F-box only protein from silkworm, Bombyx mori. Comp Funct Genomics. 2009: 416040.
15. Zhang Y, Xia Q, Xu J, Chen J, Nie Z, Wang D, Zhang W, Chen J, Zheng Q, Chen Q, Kong L, Ren X, Wang J, Lv Z, Yu W, Jiang C, Liu L, Sheng Q, Jin Y, Wu X. Aligning the proteome and genome of the silkworm, Bombyx mori. Funct Integr Genomics. 2009 Nov; 9(4):447-454.
16. Jin R, Lv Z, Chen Q, Quan Y, Zhang H, Li S, Chen G, Zheng Q, Jin L, Wu X, Chen J, Zhang Y. Safety and immunogenicity of H5N1 influenza vaccine based on baculovirus surface display system of Bombyx mori. PLoS One. 2008; 3(12):e3933.
17. He PA, Nie Z, Chen J, Chen J, Lv Z, Sheng Q, Zhou S, Gao X, Kong L, Wu X, Jin Y, Zhang Y. Identification and characteristics of microRNAs from Bombyx mori. BMC Genomics. 2008 May 28; 9:248.
18. Lingyin Kong, Zhengbing Lv, Jian Chen, Zuoming Nie, Dan Wang, Hongdan Shen, Xuedong Wang, Xiangfu Wu, Yaozhou Zhang. Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori). BBA-General subject, 2007 Dec;1770(12):1598-1604.
19. Zhang YZ, Chen J, Nie ZM, Lu ZB, Wang D, Jiang CY, He PA, Liu LL, Lou YL, Song L, Wu XF. Expression of open reading frames in silkworm pupal cDNA library. Appl Biochem Biotechnol, 2007 Mar; 136(3):327-343.
20. Wang X, Chen J, Lv Z, Nie Z, Wang D, Shen H, Wang X, Wu X, Zhang Y. Expression and Functional Analysis of CRABP from Silkworm Pupae (Bombyx mori). J Cell Biochem, 2007 Nov 1; 102(4): 970-997.