个人简历
浙江大学, 生物系统工程与食品科学学院, 教授教育经历：1. 1998.02–2002.06, Katholieke Universiteit Leuven, Belgium, 电子工程,博士, 导师: Willy Sansen2. 1992.09–1994.10, 浙江大学, 生物医学工程, 硕士, 导师: 陈裕泉3. 1987.07–1992.07, 浙江大学, 生物医学工程, 学士, 导师: 陈裕泉科研与学术工作经历：1. 2005.02-至今, 浙江大学, 生物系统工程与食品科学学院, 教授2. 2004.03-2005.02, University of Alberta, 电子系, 访问学者3. 2002.08-2004.02, Technical University of Denmark, 微电子中心, 助理教授教学与课程微电子制造与纳米技术（本科生通识课程）主讲农业工程检测技术专题讨论与实验（研究生必修课程）主讲农业与生物系统工程学（博士生必修课程）承担二分之一课程食品仪器分析实务（食品系研究生课程）负责其中生物器内容教学科研主持的重要科研项目：1. 国家自然科学基金面上项目，31571918，基于微纳磁珠的高灵敏阻抗免疫分析方法及在食物过敏原检测中应用的研究，2016/01-2019/12，主持2. 国家自然科学基金面上项目，31271617，功能离子液体厚膜电极用于阿特拉津快速检测的关键科学问题研究，2013/01-2016/12，主持3. 教育部新世纪优秀人才支持计划，NCET-07-0752，食品安全与生物传感器的研究，2008/01-2010/12，主持4. 国家自然科学基金面上项目，30670535，液态电极等离子体光谱技术及在金属离子检测中应用的研究，2007/01-2009/12，主持所获奖励 吴坚(7/14), 新型在线水质分析系统研制与产业化, 中国机械工业联合会, 科技进步, 省部一等奖, 2010.10.30 (王健; 项光宏; 王静; 韩双来; 叶华俊; 陈生龙; 吴坚; 王建华; 闻路红; 王欣; 刘中化; 杨波; 刘波; 李长喜) (科研奖励)吴坚（4/16）以生为本多元融合——依托紧密型团队的农业工程研究生培养的探索与实践 国家级教学成果一等奖 2014.9 （应义斌；蒋焕煜；徐惠荣；吴坚；成芳；泮进明；傅霞萍；谢丽娟；叶尊忠；俞永华；崔笛；李冬阳；刘湘江；邵玉芳；饶秀勤；王剑平）
研究领域
食品安全快速检测技术核酸分析纳米技术""
近期论文
Hui Wu, Cheng Qian, Cui Wu, Zhen Wang, Dacheng Wang, Zunzhong Ye, Jianfeng Ping, Jian Wu*, Feng Ji*, End-point dual specifc detection of nucleic acids using CRISPR/Cas12a based portable biosensor. Biosensors and Bioelectronics. 2020; 157:112153. (2019 IF = 10.257)Yanju Chen, Yang Liu, Ya Shi, Jianfeng Ping, Jian Wu*, Huan Chen*, Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting. TrAC Trends in Analytical Chemistry. 2020; 127: 115912. (2019 IF = 9.801)Yanju Chen, Yang Liu, Ya Shi, Jianfeng Ping, Jian Wu*, Huan Chen*, Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting. Biosensors and Bioelectronics. 2020; 153: 112049. (2019 IF = 10.257)Yuanchao Lu, Qunqing Yang, Jian Wu*, Recent advances in biosensor-integrated enrichment methods for preconcentrating and detecting the low-abundant analytes in agriculture and food samples. TrAC Trends in Analytical Chemistry 2020;128: 115914. (2019 IF = 9.801)Hui Wu, Jin-song He*, Fang Zhang, Jianfeng Ping, Jian Wu*, Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed reaction vessel: Rapid, specific and sensitive. Analytica Chimica Acta 2020;1096: 130-137. (2019 IF = 5.997)Mengyao Zhang, Jing Ye, Jin-song He*, Fang Zhang, Jianfeng Ping, Cheng Qian, Jian Wu*. Visual detection for nucleic acid-based techniques as potential on-site detection methods. Analytica Chimica Acta. 2020; 1099: 1-15. (2019 IF = 5.997)Yuanchao Lu, Xunjia Li, Jianfeng Ping, Jin-song He*, Jian Wu*, A Flexible, Recyclable, and High-Performance Pullulan-Based Triboelectric Nanogenerator (TENG). Adv. Mater. Technol. 2020; 5: 1900905. (2019 IF = 5.969)Yuanchao Lu, Yawen Zheng, Hao Zhang, Xiangxiang He, Qunqing Yang, Jian Wu*, A high performance and flexible in-plane asymmetric micro-supercapacitor (MSC) fabricated with functional electrochemical-exfoliated graphene. Journal of Electroanalytical Chemistry 2020; 866: 114169. (2019 IF = 3.807)Cheng Qian, Hui Wu, Ya Shi, Jian Wu*, Huan Chen*, Dehydrated CRISPR-mediated DNA analysis for visualized animal-bornevirus sensing in the unprocessed blood sample. Sensors & Actuators: B. Chemical. 2020; 305: 127440-127446. (2019 IF = 7.100)Mengyao Zhang, Chengzhi Liu, Ya Shi, Jun Wu, Jian Wu*, Huan Chen*, Selective endpoint visualized detection of Vibrio parahaemolyticus with CRISPR/Cas12a assisted PCR using thermal cycler for on-site application. Talanta 2020; 214: 120818. (2019 IF = 5.339)Cheng Qian, Rui Wang, Hui Wu, Fang Zhang, Jian Wu*, Liu Wang*, Uracil-Mediated New Photospacer-Adjacent Motif of Cas12a To Realize Visualized DNA Detection at the Single-Copy Level Free from Contamination. Analytical Chemistry. 2019; 91: 11362−11366. (2019 IF = 6.785)Cheng Qian, Rui Wang, Hui Wu, Feng Ji*, Jian Wu*, Nicking enzyme-assisted amplification (NEAA) technology and its applications: A review. Analytica Chimica Acta 2019;1050: 1-15. (2019 IF = 5.997)Mengyao Zhang, Ping Wu, Jun Wu, Jianfeng Ping, Jian Wu*, Advanced DNA-based methods for the detection of peanut allergens in processed food. Trends in Analytical Chemistry 2019; 114: 278-292. (2019 IF = 9.801)Cheng Qian, Rui Wang, Cui Wu, Liu Wang, Zunzhong Ye, Jian Wu*, Feng Ji*, A fast and visual method for duplex shrimp pathogens detection with high specificity using rapid PCR and molecular beacon. Analytica Chimica Acta 2018; 1040: 105-111. (2018 IF = 5.256)Liu Wang, Zhicheng Huang, Rui Wang, Yibo Liu, Cheng Qian, Jian Wu*, and Juewen Liu*, Transition Metal Dichalcogenide Nanosheets for Visual Monitoring PCR Rivaling a Real-Time PCR Instrument, ACS Appl. Mater. Interfaces 2018; 10: 4409−4418. (2018 IF = 8.456)Liu Wang, Cheng Qian, Hui Wu, Wenjuan Qian, Rui Wang, Jian Wu*, Technical aspects of nicking enzyme assistedamplification. Analyst, 2018; 143: 1444–1453. (2018 IF = 4.019)Rui Wang, Fang Zhang, Cheng Qian, Cui Wu, Zunzhong Ye, Liu Wang, Wenjuan Qian, Jianfeng Ping, Jian Wu*, Yibin Ying, Counting DNA molecules with visual segment-based readouts in minutes. Chem. Commun., 2018; 54: 1105-1108. (2018 IF = 6.164)Wenjuan Qian, Ying Lu, Youqing Meng, Zunzhong Ye, Liu Wang, Rui Wang, Qiqi Zheng, Hui Wu, Jian Wu*, Field Detection of Citrus Huanglongbing Associated with ‘Candidatus Liberibacter Asiaticus’ by Recombinese Polymerase Amplification within 15 min. J. Agric. Food Chem. 2018; 66: 5473−5480. (2018 IF = 3.571)Liu Wang, Zhicheng Huang, Yibo Liu, Jian Wu*, Juewen Liu*, Fluorescent DNA Probing Nanoscale MnO2: Adsorption, Dissolution by Thiol, and Nanozyme Activity. Langmuir 2018; 34: 3094−3101. (2018 IF = 3.683)Liu Wang, Zijie Zhang, Biwu Liu, Yibo Liu, Anand Lopez, Jian Wu*, Juewen Liu*, Interfacing DNA Oligonucleotides with Calcium Phosphate and Other Metal Phosphates. Langmuir 2018; 34: 14975−14982. (2018 IF = 3.683)Cheng Qian, Rui Wang, Hui Wu, Jianfeng Ping, Jian Wu*, Recent advances in emerging DNA-based methods for genetically modified organisms (GMOs) rapid detection. TrAC Trends in Analytical Chemistry 109 (2018) 19-31. (2018 IF = 8.428)Rui Wang, Fang Zhang, Liu Wang, Wenjuan Qian, Cheng Qian, Jian Wu*, Yibin Ying, Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3‑2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification. Anal. Chem. 2017; 89: 4413−4418. (2017 IF = 6.042)Qiqi Zheng, Yuan Chen, Kai Fan, Jian Wu*, Yibin Ying, Exploring pralidoxime chloride as a universal electrochemical probe for organophosphorus pesticides detection. Analytica Chimica Acta 2017；982: 78-83. (2018 IF = 5.123)Wenzhi Tang, Tingting Yan, Jianfeng Ping, Jian Wu,* Yibin Ying, Rapid Fabrication of Flexible and Stretchable Strain Sensor by Chitosan-Based Water Ink for Plants Growth Monitoring. Adv. Mater. Technol. 2017; 2: 1700021. (2017 IF = 4.622)Rui Wang, Xingning Xiao, Yuan Chen, Jun Wu, Wenjuan Qian, Liu Wang, Yuan Liu, Feng Ji and Jian Wu*, A loop-mediated, isothermal amplification-based method for visual detection of Vibrio parahaemolyticus within only 1 h, from shrimp sampling to results. Anal. Methods, 2017, 9, 1695–1701. (2017 IF = 2.073)Liu Wang, Cheng Qian, Wenjuan Qian, Rui Wang, Jian Wu*, Yibin Ying, A highly specific strategy for in suit detection of DNA with nicking enzyme assisted amplification and lateral flow. Sensors and Actuators B 2017; 253: 258–265. (2017 IF = 5.667)Wenzhi Tang, Jian Wu*, Yibin Ying, Yuan Liu*, Writing Sensors on Solid Agricultural Products for In Situ Detection. Anal. Chem. 2015; 87: 10703−10707. (2017 IF = 6.042)Fang Zhang, Liu Wang, Rui Wang, Yibin Ying, Jian Wu*, Simple Screening Strategy with Only Water Bath Needed for the Identification of Insect-Resistant Genetically Modified Rice. Anal. Chem. 2015; 87: 1523−1526. (2015 IF=5.886)Fang Zhang, Rui Wang, Liu Wang, Jian Wu*, Yibin Ying, Tracing phosphate ions generated during DNA amplification and its simple use for visual detection of isothermal amplified products, Chemical Communications, 2014, 50(92): 14382~14385. (2014 IF=6.834)Fang Zhang, Jian Wu*, Rui Wang, Liu Wang, Yibin Ying, Portable pH-inspired electrochemical detection of DNA amplification, Chemical Communications, 2014, 50(61): 8416~8419. (2014 IF=6.834)Jianfeng Ping, Yixian Wang, Jian Wu*, Yibin Ying, Development of an electrochemically reduced graphene oxide modified disposable bismuth film electrode and its application for stripping analysis of heavy metals in milk , Food Chemistry, 2014, 151: 65~71. (2014 IF = 3.391)Kai Fan, Xue Luo, Jianfeng Ping, Wenzhi Tang, Jian Wu*, Yibin Ying, Qingli Zhou*, Sensitive Determination of (-)-Epigallocatechin Gallate in Tea Infusion Using a Novel Ionic Liquid Carbon Paste Electrode, Journal of Agricultural and Food Chemistry, 2012, 60(25): 6333~6340. (2012 IF = 2.906)Jianfeng Ping, Yixian Wang, Jian Wu*, Yibin Ying, Feng Ji*, Determination of ascorbic acid levels in food samples by using an ionic liquid-carbon nanotube composite electrode , Food Chemistry, 2012, 135(2): 362~367. (2012 IF = 3.334)Jianfeng Ping, Yixian Wang, Kai Fan, Jian Wu*, Yibin Ying, Direct electrochemical reduction of graphene oxide on ionic liquid dopedscreen-printed electrode and its electrochemical biosensing application. Biosensors and Bioelectronics 2011; 28: 204– 209. (2018 IF = 5.602)Jian Wu*; Jing Ye, Micro flow sensor based on two closely spaced amperometric sensors, Lab on a Chip, 2005, 5(12): 1344~1347. (2005 IF=5.265)
