研究领域

何爱彬实验室致力于研究心脏发育起源与再生的细胞与分子机制。我们的研究集中在如下两个方向：1. 胚胎期心脏干细胞特化与分化的表观遗传调控机制。我们利用/发展单细胞转录组与表观遗传学技术等手段，探索心脏干细胞特化与命运决定的关键增强子组与其调控的转录因子网络。2. 实时成像结合数学模型绘制心脏发育起源/心脏再生的细胞学图谱。我们利用光片显微镜与图像图形处理技术，结合细胞谱系示踪，追踪心脏干细胞的增殖、分化、迁移和分配，绘制细胞图谱。本实验室综合运用发育生物学、表观遗传学、生物信息学、光片显微镜技术和数学建模等多学科交叉工具与方法，解决上述科学问题。

近期论文

1. Li Y, Li C, Li S, Peng Q, An NA, He A# and Li CY#. Human exonization through differential nucleosome occupancy. Proc Natl Acad Sci U S A. 2018;115:8817-8822.
2. Ai S, Yu X, Li Y, Peng Y, Li C, Yue Y, Tao G, Li C-Y, Pu WT and He A. Divergent Requirements for EZH1 in Heart Development Versus Regeneration. Circ Res. 2017;121:106-112.
3. Ai S, Peng Y, Li C, Gu F, Yu X, Yue Y, Ma Q, Chen J, Lin Z, Zhou P, Xie H, Prendiville TW, Zheng W, Liu Y, Orkin SH, Wang DZ, Yu J, Pu WT, He A. EED orchestration of heart maturation through i nteraction with HDACs is H3K27me3-independent. Elife. 2017;6.
4. He A#, Gu F, Hu Y, Ma Q, Yi Ye L, Akiyama JA, Visel A, Pennacchio LA, Pu WT#. Dynamic GATA4 enhancers shape the chromatin landscape central to heart development and disease. Nat Commun. 2014;5:4907. Co-corresponding authors.
5. Chen JY, Shen QS, Zhou WZ, Peng J, He BZ, Li Y, Liu CJ, Luan X, Ding W, Li S, Chen C, Tan BC, Zhang YE, He A#, Li CY#. Emergence, Retention and Selection: A Trilogy of Origination for Functional De Novo Proteins from Ancestral LncRNAs in Primates. PLoS Genet. 2015;11:e1005391. Co-corresponding authors.
6. He A, Shen X, Ma Q, Cao J, von Gise A, Zhou P, Wang G, Marquez VE, Orkin SH, Pu WT. PRC2 directly methylates GATA4 and represses its transcriptional activity. Genes Dev. 2012;26:37-42.
7. He A, Ma Q, Cao J, von Gise A, Zhou P, Xie H, Zhang B, Hsing M, Christodoulou DC, Cahan P, Daley GQ, Kong SW, Orkin SH, Seidman CE, Seidman JG, Pu WT. Polycomb Repressive Complex 2 Regulates Normal Development of the Mouse Heart. Circ Res. 2012;110:406-15.
8. He A, Kong SW, Ma Q, Pu WT. Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart. Proc Natl Acad Sci U S A. 2011;108:5632-7.