个人简历

简介
本实验室主要兴趣在于：
　　多年来，本实验室的工作得到国际同行的认可，与欧洲的几个实验室建立并维持着卓有成效的合作关系，如法国巴斯德研究所、英国帝国理工学院、英国John Innes研究所、爱尔兰Carlow理工学院、爱尔兰国立大学Cork学院、西班牙国家生物技术中心、德国比勒菲尔德大学等。主要得到国家自然科学基金、国家科技部“863”、“973”项目基金、中法先进合作项目、国家教育部基金资助。主要工作包括：大肠杆菌及相关细菌中的基因调控网络，尤其是碳代谢和氮代谢的调控偶联；大肠杆菌及相关细菌中的基因调控机理；植物与微生物相互作用的分子生物学及功能基因组学研究；生物修复领域的研究（功能基因的分离）；在大肠杆菌及相关细菌中大量表达外源基因技术等。取得的主要成就有，发现原核基因表达调控中碳代谢及氮代谢之间的新的偶联作用及其分子机理；首次发现“下游激活序列”对固氮基因的调控作用；获得了新型高效抗草苷膦基因，在核酸水平上与已知基因没有同源性，在氨基酸水平上也只有37％左右的同源性，具有与受专利保护的EPSPS基因保守区不同的序列特征。目前世界上70％的转基因植物含有抗除草剂基因，其中60％是草甘膦耐受型EPSPS基因,具有很大的应用前景。

研究领域

多年来，本实验室的工作得到国际同行的认可。主要得到国家自然科学基金、国家科技部“863”、“973”项目基金、国家教育部基金资助、中法先进合作项目、Human Frontier Science Program等项目资助。主要工作包括：大肠杆菌及相关细菌中的基因调控网络，尤其是碳代谢和氮代谢的调控偶联；大肠杆菌及相关细菌中的基因调控机理；植物与微生物相互作用的分子生物学及功能基因组学研究；生物修复领域的研究（功能基因的分离）；合成生物学及生物固氮；大肠杆菌定量生物学研究等。取得的主要成就有，发现原核基因表达调控中碳代谢及氮代谢之间的新的偶联作用及其分子机理；发现DNA物理特性参与基因表达调控（该成果被国际知名学术网站Faculty of 1000 推荐）；提出了激活蛋白－启动子DNA－σ54-RNA聚合酶所形成的激活复合体的“三明治”结构模型（该成果被国际知名学术网站Faculty of 1000 推荐；使用合成生物学方法，我们使用T7 RNA聚合酶表达系统替代原有的σ54 RNA聚合酶对固氮基因簇的转录调控，绕开了原有固氮基因簇对转录调控系统中14种调控蛋白的依赖，为最终实现固氮基因向真核系统的转移打下坚实的基础；以肺炎克氏杆菌钼铁固氮基因簇为底盘，成功的在大肠杆菌中构建了杂合的铁铁固氮酶体系，从而在不损失酶活的前提下，成功构建了杂合的只含有10个结构基因的最小铁铁固氮酶体系。这一体系的建立，成功的绕开了我国缺乏钼元素，及植物学界关于线粒体、叶绿体内钼元素缺乏等问题，为最终实现固氮基因向真核系统的转移打下坚实的基础。"研究领域：大肠杆菌及相关细菌中的基因调控网络、大肠杆菌及相关细菌中的基因调控机理、植物与微生物相互作用的分子生物学及功能基因组学研究、生物修复领域的研究。"

近期论文

1. Yang, J., Xie, X., Xiang, N., Dixon, R.,* and Wang, Y.P.* (2018) A polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology. Proc Natl Acad Sci U S A. 201804992; DOI: 10.1073/pnas.1804992115 [Highlighted by Stefan Burén, Gema Ló pez-Torrejón, and Luis M. Rubio (2018) Extreme bioengineering tomeet the nitrogen challenge Proc Natl Acad Sci U S A. doi/10.1073/pnas.1812247115] 2. Zhu, M., Dai, X., Guo, W., Ge, Z., Yang, M., Wang, H., and Wang, Y.P.* (2017) Manipulating bacterial cell cycle and cell size by titrating the expression of ribonucleotide reductase. mBio 8: e01741-17.. 3. Yang, J., Xie, X. Yang, M. Dixon, R.* and Wang, Y.P.* Modular electron transport chains from eukaryotic organelles function to support nitrogenase activity. Proc Natl Acad Sci U S A. 2017, 114: 3009-3011. doi:10.1073/pnas.1620058114 [Highlighted by Vicente, E.J. and Dean, D.R. Keeping the nitrogen-fixation dream alive. Proc Natl Acad Sci U S A. 2017, 114 (12): 3009-3011, doi:10.1073/pnas.1701560114. And by Good A. (2018) Toward nitrogen-fixing plants. Science 359 (6378): 869-870, DOI: 10.1126/science.aas8737]. It has been compiled by the Royal Society of Biology (UK) as one of The Big Biology Breakthroughs of 2017. https://www.rsb.org.uk/about-us/mysociety/158-biologist/features/1881-big-biology-breakthroughs-2017 4. Dai, X., Zhu, M., Warren, M., Balakrishnan, R., Patsalo, V., Okano, H., Williamson, J.R., Fredrick, K., Wang, Y.P.* Hwa, T.* Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth. Nature Microbiology 2016, 2:16231. doi: 10.1038/nmicrobiol.2016.231 5. Zhu, M., Dai, X.,* and Wang, Y.P.* Real time determination of bacterial in vivo ribosome translation elongation speed based on LacZ complementation system. Nucleic Acids Res. 2016, 44 (20): e155. doi: 10.1093/nar/gkw698. 6. Tian, Z.X*., Yi, X.X., Cho, A., O`Gara, F., and Wang, Y.P.* CpxR activates MexAB-OprM efflux pump expression and enhances antibiotic resistance in both laboratory and clinical nalB-type isolates of Pseudomonas aeruginosa. PLOS Pathogens 2016, 12(10):e1005932. doi: 10.1371/journal.ppat.1005932. 7. Wang, J., Yan, D., Dixon, R.*, and Wang, Y.P.* Deciphering the principles of bacterial nitrogen dietary preferences: a strategy for nutrient containment. mBio 2016, 7(4):e00792-16. doi:10.1128/mBio.00792-16. 8. Yang, Y., Darbari, V.C, Zhang, N., Lu, D., Glyde, R., Wang, Y.P., Winkelman, J., Gourse, R.L., Murakami, K.S., Buck, M., Zhang, X.*, Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies. Science, 2015, 349: 882-885 doi: 10.1126/science.aab1478 9. Basan, M., Zhu, M., Dai, X., Warren, M., Sévin, D., Wang, Y.P., Hwa, T.*, Inflating bacterial cells by increased protein synthesis. Molecular Systems Biology, 2015, 11: 836. 10. Liang, J.L., Yong Nie, Y., Wang, M., Xiong, G., Wang, Y.P., Maser, E., and Wu, X.L.*, Regulation of alkane degradation pathway by a TetR family repressor via an autoregulation positive feedback mechanism in a Gram-positive Dietzia bacterium. Mol. Microbiol., 2015, 99(2):338-59. doi: 10.1111/mmi.13232. 11. Tian, C., Jinren Ni, J.*, Chang, F., Liu, S., Xu, N., Sun, W., Xie, Y., Guo, Y., Ma, Y., Yang, Z., Dang, C., Huang, Y., Tian, Z., and Wang, Y.P, Bio-Source of di-n-butyl phthalate production by filamentous fungi. Scientific Report, 2015, 6:19791. doi: 10.1038 12. Liu, J., Wen, J., Yang, J., Yang, Y., Wei, X., Zhang, X.* and Wang, Y.P.*, Mutational analysis of dimeric linkers in peri- and cytoplasmic domains of histidine kinase DctB reveal their functional roles in signal transduction, Open Biology, 2014, 4: 140023. 13. Zhang, Y., Jiang, F., Tian, Z., Huo, Y., Sun, Y., and Wang, Y.P.*, CRP-Cyclic AMP Dependent Inhibition of the Xylene-Responsive σ54-Promoter Pu in Escherichia coli, PLoS ONE 2014, 9(1): e86727. 14. Dai, X., Zhu, M., and Wang, Y.P.*, Circular permutation of E. coli EPSP synthase: increased inhibitor resistance, improved catalytic activity, and an indicator for protein fragment complementation , Chem. Commun., 2014, 50 (15): 1830 – 1832. 15. Yang, J., Xie, X., Wang, X., Dixon, R.*, and Wang, Y.P.*, Reconstruction and minimal gene requirement for the alternative iron-only nitrogenase system in Escherichia coli., Proc Natl Acad Sci U S A., 2014, 111(35): E3718-E3725 [Highlighted by Vicente, E.J. and Dean, D.R. Keeping the nitrogen-fixation dream alive. Proc Natl Acad Sci U S A. 2017, 114 (12): 3009-3011, doi:10.1073/pnas.1701560114] 16. Zhou, Y., Kolb, A., Busby, S.J.W., and Wang, Y.P.*, Spacing requirements for bacterial Class I transcrIption activation are set by promoter elements., Nucleic Acids Res., 2014, 42(14): 9209-16. 17. You, C., Okano, H., Hui, S., Zhang, Z., Kim, M., Gunderson, C.W., Wang, Y.P., Lenz, P., Yan, D., and Hwa, T.*, Coordination of bacterial proteome with metabolism by cAMP signalling, Nature, 2013, 500: 301-306 18. Wang, X., Yang, J., Chen, L., Cheng, Q., Dixon, R.*, and Wang, Y.P.*, Using Synthetic Biology to Distinguish and Overcome Regulatory and Functional Barriers Related to Nitrogen Fixation, PLoS ONE , 2013 , 8(7): e68677 19. Aogain, M.M., Mooij, M.J., McCarthy, R.R., Eimear Plower, E., Wang, Y.P., Tian, Z.X., Dobson, A.D.W., Morrissey, J.P. Claire Adams, C., and O`Gara, F.*, The non-classical ArsR-family repressor PyeR (PA4354) modulates biofilm formation in Pseudomonas aeruginosa, Microbiology , 2012, 158: 2598-2609