在DNA测序研究方面做出杰出贡献。1978年他发现梯度电场抵抗核酸分子扩散效应，导致DNA顺序的可读量增加30%以上，在国际上被广泛地应用。他创建了单链DNA双向测定的方法。他创建了高温DNA测序体系。他从嗜热脂肪芽孢杆菌中成功的分离出耐高温高保真高进行性的BstDNA聚合酶，利用该酶，成功地消除了长期未能解决的DNA顺序测定由二级结构造成的“堆积效应”。该酶已获美国、欧洲、日本等国的专利。洪国藩的有关DNA研究成果已被收录在美国出版的权威著作《Molecular Cloning》和《Methods in Enzymology》之中
研究领域
Small RNA是当前国际研究热点之一，本研究组研究共生生物体内的Small RNA世界
近期论文
Yinghua Zhang and Guofan Hong. Evidences of Hfq associates with tryptophanase and affects extracellular indole levels. Acta Biochim Biophys Sin (Shanghai). 2009, 41:709-717.Yinghua Zhang and Guofan Hong. Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae. Acta Biochim Biophys Sin (Shanghai). 2009, 41: 719-730.Bihe Hou, Fengqing Li, Xiao’er Yang and Guofan Hong. A small functional intramolecular region of NodD was identified by mutation. Acta Biochim Biophys Sin (Shanghai). 2009, 41: 822-830.Bihe Hou, Fengqing Li, Xiao’er Yang and Guofan Hong. The properties of NodD were affected by mere variation in length within its hinge region. Acta Biochim Biophys Sin (Shanghai). 2009, 41:936-971.Li F, Hou BH, Lei C, Yao ZJ, Hong GF. In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer. Acta Biochim Biophys Sin (Shanghai). 2008, 40: 783-789.Fengqing Li, Bihe Hou, and Guofan Hong. Symbiotic plasmid is required for NolR to fully repress nodulation genes in Rhizobium leguminosarum A34. Acta Biochim Biophys Sin (Shanghai). 2008, 40:901-907.Chen XC, Feng J, Hou BH, Li FQ, Li Q, Hong GF. Modulating DNA bending affects NodD-mediated transcriptional control in Rhizobium leguminosarum. Nucl Acids Res. 2005, 33: 2540-8.
