黎明
近期热点
资料介绍
个人简历
黎明,男,博士,教授,硕士生导师。2003年09至今一直天津科技大学生物工程学院、教育部工业发酵微生物重点实验室、天津市工业微生物重点实验从事教学和科研工作。曾在美国伦斯勒理工学院访学一年。科研项目:参与或主持的项目多项,近五年的主要科研项目如下:1、国家自然科学基金面上项目,No.21176190、谷氨酸棒杆菌生物合成尸胺及其代谢调控研究。主持人2、天津市科技支撑计划项目,No.11ZCKFSY00900、高活力碱性果胶酶的规模发酵技术及酶制剂的开发。主持人3、天津市应用基础及前沿技术研究计划项目,No.11JCYBJC09600、高产α-葡萄糖转苷酶黑曲霉工程构建及发酵优化。主持人。4、国家863项目,NO.2007AA02Z212、热稳定低温碱性蛋白酶的定向改造。第3完成人。5、国家科技部项目子项目:食品微生物基因组改造,第三参加人。6、国家科技部项目子项目:大宗食品发酵安全低碳加工关键技术研究-07.第二参加人。7、教育部项目:大肠杆菌中蒜氨酸生物合成途径的构建及其代谢流研究。第二参加人。8、天津科技大学科研平台项目,高产柠檬酸大肠杆菌工程菌的构建。主持人。主要著作:参加编写《微生物实验技术》,中国轻工业出版社,2005.研究领域
[1]微生物与生化药学:基因工程药物,酶制剂的开发与应用;[2]工业微生物育种:基于代谢工程策略,对微生物进行改造,从而实现高附加值化学品和平台化合物的微生物合成与生产;[3]发酵工程:微生物代谢工程与发酵。近期论文
1.WenqinHe,YuanyuanZhu,AbhijeetShirke,XiaojunSun,JianLiu,RichardA.Gross,MattheosA.G.Koffas*,RobertJ.Linhardt*,MingLi*.Expressionofchondroitin-4-O-sulfotransferaseinEscherichiacoliandPichiapastoris.AppliedMicrobiologyandBiotechnology(ApplMicrobiolBiotechnol).2017,101:6919-6928.2.杨田,随树珍,刘逸寒,黎明*.大麻纤维微生物脱胶菌株的筛选、鉴定及其应用.天津科技大学学报,2017,32(4):24-29.3.QingHao,XiaoguangLiu,GuozhongZhao*,LuJiang,MingLi,BinZeng*.Recombinantexpression,purification,andcharacterizationofanacyl-CoAbindingproteinfromAspergillusoryzae.BiotechnologyLetters(BiotechnolLett).2016,38:519–5254.RunWei,Xiao-meiLiang1,Mei-juanXuan,Hao-yuYuan,Fu-pingLuandMingLi*.ConstructionoftheEscherichiacoli-BacillussubtilisShuttleVectorpBE2RandIdentificationoftheCriticalResiduesInvolvedintheAutoprocessingofthePropeptideoftheAlkalineProtease.InternationalConferenceonAppliedBiotechnology(ICAB2016),November25-27,2016.Tianjin,China.5.Mei-juanXuan,Jian-zhongZhang,Hao-yuYuan,RunWei,Fu-pingLuandMingLi*.OptimizationofProcessforOpticalResolutionofDL-pantolactoneUsingImmobilizedD-lactonohydrolase.InternationalConferenceonAppliedBiotechnology(ICAB2016),November25-27,2016.Tianjin,China.6.黎明,唐奇,李东霞,李杨,胡顿吉,随树珍,路福平.谷氨酸棒杆菌发酵生产1,5-戊二胺的代谢流分析.天津科技大学学报.2015,30(1):9-137.MingLi,DongxiaLi,YunyanHuang,MengLiu,HongxinWang,QiTang,FupingLu.ImprovingthesecretionofcadaverineinCorynebacteriumglutamicumbycadaverine-lysineantiporter.JournalofIndustrialMicrobiology&Biotechnology.2014,41(4):701-709.8.李东霞,黎明*,王洪鑫,王舒雅,路福平.生物法合成戊二胺研究进展.生物工程学报.2014,30(2):161-174.9.MingLI,LiyingZHOU,MengLIU,YunyanHUANG,XinSUN,FupingLU.TheConstructionofEngineeringStrainproducinghighyieldofα-transglucosidaseviaAgrobacteriumtumefaciens-mediatedTransformationofAsperillusniger.BioscienceBiotechnologyandBiochemis.2013,77(9):1860-186610.MingLi,DongxiaLi,YunyanHuang,TaoNiu,MengLiu,LiyingZhou,XinSun,FupingLu.ConstructionofRecombinantCorynebacteriumglutamicumProducingCadaverinebyOneStepMethod.InternationalJournalofAdvancementsinComputingTechnology.2013,5(8):619-629.11.TongleiShi,AiqunYu,MingLi,MengZhang,LaijunXing,MingchunLi.IdentificationandcharacterizationofanovelC20-elongasegenefromthemarinemicroalgae,Pavlovaviridis,anditsuseforthereconstitutionoftwopathwaysoflong-chainpolyunsaturedfattyacidsbiosynthesisinSaccharomycescerevisiae.BiotechnologyLetters.2013,35(8):1271-1282.12.MingLi,XiuyuanOu,DongshengWei,XiangdongYang,DongquanGuo,XueyanQian,LaijunXing,MingchunLi.CloningandidentificationofanovelΔ9-specificpolyunsaturatedfattyacidelongasevariantfromDHA-producingIsochrysisgalbanaH29.BiotechnologyandBioprocessEngineering.2012,17:22-32.13.TongleiShi,AiqunYu,MingLi,XiuyuanOu,LaijunXingandMingchunLi.IdentificationofanovelC22-∆4-producingdocosahexaenoicacid(DHA)specificpolyunsaturatedfattyaciddesaturasegenefromIsochrysisgalbanaanditsexpressioninSaccharomycescerevisiae.BiotechnologyLetters.2012,34:2265-227414.YihanLiu,GuanqunChen,JianlingWang,YujieHao,MingLi,YuLi,BoHu,FupingLu.EfficientexpressionofanalkalinepectatelyasegenefromBacillussubtilisandthecharacterizationoftherecombinantprotein.BiotechnolLett,2012,34:109–11515.黎明,刘萌,黄云雁,周丽颖,孙昕,路福平.根癌农杆菌介导的黑曲霉遗传转化体系的建立及优化.中国生物工程杂志.2012,32(1):56-63.16.MingLi,XiuyuanOu,XiangdongYang,DongquanGuo,XueyanQian,LaijunXing,andMingchunLi.IsolationofanovelC18-Δ9polyunsaturatedfattyacidspecificelongasegenefromDHA-producingIsochrysisgalbanaH29anditsuseforthereconstitutionofthealternativeΔ8pathwayinSaccharomycescerevisiae.BiotechnologyLetters.2011,33(9):1823-1830 相关热点
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