个人简历
Education Experience 2003-2008 Ph. D. Organic Chemistry Nankai Univ 1999-2003 Bachelor of Science Chemistry Nankai Univ Professional Experience 2009-2014 Postdoc University of California, Berkeley 2014-2016 Ass Project Scientist University of California, Berkeley 2016- Associate Prof Tianjin Univ
研究领域
Our group research interest lies in investigating basic mechanism of bio-active molecular (including nitric oxide, hydride and methyl et al.) transfer reaction in enzyme or in solution, with a goal of 1) design of more efficient chemical catalysis as well as the designed enzyme; 2) rational design of corresponding inhibitor/drug basing on the mechanism exploration. This inspiring research area requires the combination the application of physical organic chemistry, biochemistry, chemical biology and molecular biology. The most recently work refers to the understanding the role of compaction in methyl transfer reactions with the target of finding how the molecular motion in enzymes would affect the catalytic ability in the methyl transfer reaction. This study about the methyl transfer system had/will extended from catechol-O-methyltransferase (COMT) to glycine-N-methyltransferase (GNMT), Nicotinamide N-Methyltransferase (NNMT) and DNA\\RNA demethylation with various experimental approaches such kinetic isotope effect, binding isotope effect, time-resolved spectrometers, hydrogen deuterium exchange with mass spectrometry (HDX-MS) as well as computational simulation.
近期论文
2018 Activity-Related Microsecond Dynamics Revealed by Temperature-Jump Forster Resonance Energy Transfer Measurements on Thermophilic Alcohol Dehydrogenase Vaughn, Morgan B.; Zhang, Jianyu; Spiro, Thomas G.; et al. Journal of the American Chemical Society 140 2016 CH···O Interactions Are Not the Cause of Trends in Reactivity and Secondary Kinetic Isotope Effects for Enzymatic SN2 Methyl Transfer Reactions Zhang, Jianyu; Klinman, Judith P. Biorxiv Preprint 2016 Convergent Mechanistic Features between the Structurally Diverse N- and O-Methyltransferases: Glycine N-Methyltransferase and Catechol O-Methyltransferase Zhang, J. Y.; Klinman, J. P. Journal of the American Chemical Society 138 2016 How Large Should the QM Region Be in QM/MM Calculations? The Case of Catechol O-Methyltransferase Kulik, Heather J.; Zhang, Jianyu; Klinman, Judith P.; et al. Journal of Physical Chemistry B 120 2015 High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-L-methionine Zhang, Jianyu; Klinman, Judith P. Analytical Biochemistry 476 2015 How large should the QM region be in QM/MM calculations? The case of catechol O-methyltransferase Kulik, Heather J; Zhang, Jianyu; Klinman, Judith P; et al. arXiv preprint 2015 Mediation of donor-acceptor distance in an enzymatic methyl transfer reaction Zhang, Jianyu; Kulik, Heather J.; Martinez, Todd J.; et al. Proceedings of the National Academy of Sciences of the United States of America 112 2011 Enzymatic Methyl Transfer: Role of an Active Site Residue in Generating Active Site Compaction That Correlates with Catalytic Efficiency Zhang, Jianyu; Klinman, Judith P. Journal of the American Chemical Society 133 2007 Mechanism and driving force of NO transfer from S-nitrosothiol to cobalt(II) porphyrin: A detailed thermodynamic and kinetic study Zhu, Xiao-Qing; Zhang, Jian-Yu; Cheng, Jin-Pei Inorganic Chemistry 46 2006 A facile experimental method to determine the hydride affinity of polarized olefins in acetonitrile Zhu, XQ; Zhang, M; Liu, QY; et al. Angewandte Chemie-International Edition 45 2006 Mechanism of NO transfer from NO-donors (SNAP and G-MNBS) to ferrous tetraphenylporphyrin in CH3OH Zhu, Xiao-Qing; Zhang, Jian-Yu; Mei, Lian-Rui; et al. Organic Letters 8 2006 Negative kinetic temperature effect on the hydride transfer from NADH analogue BNAH to the radical cation of N-benzylphenothiazine in acetonitrile Zhu, Xiao-Qing; Zhang, Jian-Yu; Cheng, Jin-Pei Journal of Organic Chemistry 71
