个人简历

Education Experience 2001-2006 Ph. D. School of Medicine, Cell and Molecular Biology, Ge University of Pennsylvania, PA, USA Professional Experience 2008-2013 Research Scientist, Postdoctoral Fellow Massachusetts Institute of Technology 2013-2014 Senior Scientist R&D Systems Inc 2014-2018 Associate Professor Tianjin University 2018-2018 Professor Tianjin University

研究领域

The Zhang lab identifies and characterizes new enzymes and new metabolic pathways in nature using a combination of bioinformatics, genetic, biochemical and biophysical methods.  In particular, the Zhang lab has a long term interest in metal trafficking, metalloenzymes. and their catalytic mechanisms. Other projects in the Zhang lab include synthetic biology, and immuno-based human disease diagnosis.

近期论文

Xing M#, Wei Y#, Zhou Y, Zhang J, Lin L, Hu Y, Hua G, Nanjaraj A.N.,Liu D, Wang F, Guo C, Tong Y, Li M, Liu Y, Ang E, Zhao H*, Yuchi Z*, Zhang Y*. Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria. Nat. Commun. DOI: 10.1038/s41467-019-09618-8. Zhang J#, Nanjaraj A.N. #, Lin L, Yuchi Z*, Zhang Y*. Structure of glycerol dehydrogenase (GldA) from Escherichia coli. ACTA CRYSTALLOGR F.2019, Mar ; DOI: 10.1107/S2053230X19000037 Zhou Y#, Wei Y#, Lin L, Xu T, Ang E, Zhao H*, Yuchi Z*, Zhang Y*. Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca. Biochem J. 2019 ,Feb; DOI:10.1042/BCJ20190005. Nanjaraj A.N., Hu Y, Li P, Yuchi Z, Chen Y*, Zhang Y*. Cloning and expression of a non-ribosomal peptide synthetase to generate blue rose. ACS Synth. Biol. DOI: 10.1021/acssynbio.8b00187 Liu D#, Wei Y#, Liu X, Zhou Y, Jiang L, Yin J, Wang F, Hu Y, Nanjaraj A.N., Liu Y, Ang E, Zhao S*, Zhao H*, Zhang Y*. Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole. Nature Comm. 2018 Oct 11;9(1):4224. doi: 10.1038/s41467-018-06627-x. Guo C#, Hu Y#, Yang C, Nanjaraj Urs AN, Zhang Y* Developing a colorimetric assay for Fe(II)/2-oxoglutarate-dependent dioxygenase. Anal Biochem. 2018 May 1;548:109-114. Hua G#, Hu Y#, Yang C, Liu D, Mao Z, Zhang L, Zhang Y* Characterization of santalene synthases using an inorganic pyrophosphatase coupled colorimetric assay. Anal Biochem. 2018 Apr 15;547:26-36. Zhang Y, Yang C, Dancis A, Nakamaru-Ogiso E. EPR studies of wild type and mutant Dre2 identify essential [2Fe–2S] and [4Fe–4S] clusters and their cysteine ligands. J Biochem. 2017 Jan;161(1):67-78. Zhang Y#, Li H#, Zhang C, An X, Liu L, Stubbe J, Huang M. The Conserved Electron Donor Complex Dre2-Tah18 Is Required for Ribonucleotide Reductase Metallocofactor Assembly and DNA Synthesis. Proc Natl Acad Sci U S A. 2014 Apr 29; 111(17): E1695-704. Yoon H, Knight SA, Pandey A, Pain J, Zhang Y, Pain D, Dancis A. Frataxin-bypassing Isu1: characterization of the bypass activity in cells and mitochondria. Biochem J. 2014 Apr 1;459(1):71-81. Zhang Y, Liu L, Stubbe J. Huang M. Investigation of in vivo roles of the carboxyl terminal tails of the small subunit (ββ’) of S. cerevisiae ribonucleotide reductase: contribution to cofactor formation and inter-subunit association within the active holoenzyme. J Biol Chem. 2013 May 17;288(20):13951-9. Selected as the “Papers of The Week”， highlighted at http://www.jbc.org/content/288/20/13960/suppl/DCAuthor_profile Zhang Y, Liu L, Wu X, An X, Stubbe J , Huang M. Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2: requirement of Rnr4 and contribution of Grx3/4 and Dre2. J Biol Chem. 2011. Dec 2;286(48):41499-509. Selected as the “Papers of The Week”, highlighted at http://www.jbc.org/content/286/48/41499/suppl/DCAuthor_profile http://www.asbmb.org/asbmbtoday/asbmbtoday_article.aspx?id=14939 Zhang Y, Stubbe J, Bacillus subtilis Class Ib Ribonucleotide Reductase Is a Dimanganese(III)-Tyrosyl Radical Enzyme. Biochemistry. 2011 Jun 28;50(25):5615-23. Mühlenhoff U, Molik S, Godoy JR, Uzarska MA, Richter N, Seubert A, Zhang Y, Stubbe J, Pierrel F, Herrero E, Lillig CH, Lill R. Cytosolic monothiol glutaredoxins function in intracellular iron sensing and trafficking via their bound iron-sulfur cluster. Cell Metab. 2010 Oct 6;12(4):373-85. Yoon H, Zhang Y, Pain J, Lyver ER, Lesuisse E, Pain D, Dancis A. Rim2, pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria. Biochem J. 2011 Nov 15;440(1):137-46. Zhang Y, Lyver, ER, Amutha B, Ohnishi, T, Pain, D, and Dancis, A. Dre2, a conserved eukaryotic protein with both [2Fe-2S] and [4Fe-4S] clusters, functions in cytosolic iron-sulfur protein biogenesis. Mol Cell Biol. 2008 Sept. 28 (18) 5569-82. Zhang Y, Lyver, ER, Knight, SAB, Pain, D, Lesuisse, E, and Dancis, A. Mrs3p, Mrs4p, and frataxin provide iron for Fe-S cluster synthesis in mitochondria. J Biol Chem. 2006 Aug 11;281(32):22493-502. Zhang Y, Lyver, ER, Knight, SAB, Lesuisse, E, and Dancis, A. Frataxin and mitochondrial carrier proteins, Mrs3p and Mrs4p, cooperate in providing iron for heme synthesis. J Biol Chem. 2005 May 20; 280 (20):19794-807. He Y, Alam SL, Proteasa SV, Zhang Y, Lesuisse E, Dancis A, and Stemmler T.L. Yeast frataxin solution structure, iron binding, and ferrochelatase interaction. Biochemistry. 2004 Dec 28;43(51):16254-62. Deshmukh M, May M, Zhang Y, Gabbert KK, Karberg KA, Kranz RG, and Daldal F. Overexpression of ccl1-2 can bypass the need for the putative apocytochrome chaperone CycH during the biogenesis of c-type cytochromes. Mol. Microbiology 2002 Nov 46(4)1069-1080.