个人简历
Education Experience 2000-2004 Bachelor of Science Bioengineering Tianjin University of Science and Technology 2005-2010 Ph. D. Microbiology Nankai University 2008-2009 Ph. D. Protein Engineering VTT Technical Research Centre of Finland 2010-2014 Postdoctoral Cell Biology New York University Professional Experience 2004-2016 Tianjin University
研究领域
My research is focused on two specific areas, one is hydrophobin and another one is biology and disease of urothelium. For the hydrophobin study, my research is involved but not limited to: 1) Study protein-protein interaction and protein assembly in vitro and in vivo. 2) Protein expression, purification and characterization in both prokaryotic and eukaryotic systems. 3) Hydrophobin based drug and gene delivery systems. 4) Hydrophobin based chip and sensors. 5) Antibody production and purification. 6) Large-scale high-density fermentation and downstream separation. For the urothelium study, my research is involved but not limited to: 1) Study the urothelial disease like bacterial urinary tract infection and bladder. 2) study of ATP release channel of urothelium in mouse model. 3) Drug release and delivery by adenovirus and liposome in urothelium. 4) Characterization and function study of lipid raft proteins. 5) Study the stem cell of the urothelium.
近期论文
2014 Crystallization and preliminary crystallographic study of Porcine epidemic diarrhea virus main protease in complex with an inhibitor Tan, Yusheng; Wang, Fenghua; Chen, Xia; et al. Acta Crystallographica Section F-Structural Biology Communications 70 2013 Poly(epsilon-caprolactone) modified with fusion protein containing self-assembled hydrophobin and functional peptide for selective capture of human blood outgrowth endothelial cells Huang, Yujian; Zhang, Suai; Niu, Baolong; et al. Colloids and Surfaces B-Biointerfaces 101 2012 MAL facilitates the incorporation of exocytic uroplakin-delivering vesicles into the apical membrane of urothelial umbrella cells Zhou, Ge; Liang, Feng-Xia; Romih, Rok; et al. Molecular Biology of the Cell 23 2011 Immobilization of anti-CD31 antibody on electrospun poly(epsilon-caprolactone) scaffolds through hydrophobins for specific adhesion of endothelial cells Zhang, Min; Wang, Zhexiang; Wang, Zefang; et al. Colloids and Surfaces B-Biointerfaces 85 2010 Adsorption of Protein on Hydrophobin Surface Driven by Electrostatic Force Wang Ze-Fang; Huang Yu-Jian; Niu Bao-Long; et al. Chemical Journal of Chinese Universities-Chinese 31 2010 Characterization and application of hydrophobin-dispersed multi-walled carbon nanotubes Wang, Zefang; Wang, Yanyan; Huang, Yujian; et al. Carbon 48 2010 Expression and characterization of a Grifola frondosa hydrophobin in Pichia pastoris Wang, Zefang; Feng, Shuren; Huang, Yujian; et al. Protein Expression and Purification 72 2010 Hydrophilic modification of polystyrene with hydrophobin for time-resolved immunofluorometric assay Wang, Zefang; Huang, Yujian; Li, Shan; et al. Biosensors & Bioelectronics 26 2010 Mechanisms of Protein Adhesion on Surface Films of Hydrophobin Wang, Zefang; Lienemann, Michael; Qiau, Mingqiang; et al. Langmuir 26 2010 Prokaryotic expression, purification, and polyclonal antibody production of a hydrophobin from Grifola frondosa Wang, Zefang; Feng, Shuren; Huang, Yujian; et al. Acta Biochimica Et Biophysica Sinica 42 2008 Protein HGFI from the edible mushroom Grifola frondosa is a novel 8 kDa class I hydrophobin that forms rodlets in compressed monolayers Yu, Lei; Zhang, Baohua; Szilvay, Geza R.; et al. Microbiology-Sgm 154 Use of fungal hydrophobin having a property capable of self-assembling into amphoteric protein film at the two interfaces of the hydrophobin, or self-assemble the fungal hydrophobin, for making biochips QIAO M; YU L; WANG Z; et al.
