个人简历

教育背景： 2002/09– 2006/06，湖南师范大学，生命科学学院，基地班，学士 2006/09– 2012/12，湖南师范大学，生命科学学院，微生物学，博士 2008/12 – 2010/11，德国德累斯顿工业大学，基因组系，联培博士 工作经历： 2013/05 – 2017/05，山东大学，生命科学学院，博士后 2016/03 – 2018/06，山东大学，微生物技术国家重点实验室，科研助理 2018/07 – 至今， 山东大学，微生物技术研究院/国家重点实验室，教授

研究领域

1.微生物合成生物学 2.DNA重组技术开发 微生物药物生物合成途径优化 3.多效植物促生（固氮、杀虫）细菌的构建及改良

近期论文

1.Song, C.1, Luan, J.1, Cui, Q., Duan, Q., Li, Z., Gao, Y., Li, R., Li, A., Shen, Y., Li, Y., Stewart, A. F., Zhang, Y.*, Fu, J.* &Wang, H.*, Enhanced heterologous spinosad production from a 79-kb synthetic multi-operon assembly,ACS Synth. Biol., 2019, 8(1), 137-147. 2.Zhang, Y.1, Liu, R.1, Tian, K.1, Wang, Z.1, Yang, X., Gao, D., Zhang, Y., Fu, J.*,Wang, H.*& Zhao, J.*, Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4,Emerg. Microbes Infect., 2018, 7(1), 199. 3.Jing, X.1, Cui, Q.1, Li, X., Yin, J., Ravichandran, V., Pan, D., Fu, J., Tu, Q.,Wang, H.*, Bian, X.* & Zhang, Y.*, EngineeringPseudomonas protegensPf-5 to improve its antifungal activity and nitrogen fixation,Microb. Biotechnol., 2018, Epub ahead of print. 4.Wang, H.1, Li, Z., Jia, R., Yin, J., Li, A., Xia, L., Yin, Y., Muller, R., Fu, J.*, Stewart, A. F.* & Zhang, Y.*, ExoCET: exonucleasein vitroassembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes,Nucleic Acids Research, 2018, 46(5), e28. 5.Wang, H.1, Li, Z.1, Jia, R., Hou, Y., Yin, J., Bian, X., Li, A., Muller, R., Stewart, A. F.*, Fu, J.* & Zhang, Y.*, RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression,Nature Protocols, 2016, 11(7), 1175-1190. 6.Wang, H.1, Bian, X., Xia, L., Ding, X., Muller, R., Zhang, Y.*, Fu, J.* & Stewart, A. F.*, Improved seamless mutagenesis by recombineering usingccdBfor counterselection,Nucleic Acids Research, 2014, 42(5), e37. 7.Wang, H.1, Yao, T., Cai, M., Xiao, X., Ding, X. & Xia, L.*, A genome walking strategy for the identification of nucleotide sequences adjacent to known regions,Biotechnology letters, 2013, 35(2), 279-284. 8.Fu, J.1, Bian, X.1, Hu, S.,Wang, H., Huang, F., Seibert, P. M., Plaza, A., Xia, L., Muller, R.*, Stewart, A. F.* & Zhang, Y.*, Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting,Nature Biotechnology, 2012, 30(5), 440-446. 9.Rostovskaya, M.1, Fu, J., Obst, M., Baer, I., Weidlich, S.,Wang, H., Smith, A. J. H., Anastassiadis, K.* & Stewart, A. F.*, Transposon-mediated BAC transgenesis in human ES cells,Nucleic Acids Research, 2012, 40(19), e150. 10.Zhang, W.1, Fu, J.1, Liu, J.,Wang, H., Schiwon, M., Janz, S., Schaffarczyk, L., von der Goltz, L., Ehrke-Schulz, E., Domer, J., Solanki, M., Boehme, P., Bergmann, T., Lieber, A., Lauber, C., Dahl, A., Petzold, A., Zhang, Y.*, Stewart, A. F.* & Ehrhardt, A.*, An engineered virus library as a resource for the spectrum-wide exploration of virus and vector diversity,Cell Reports, 2017, 19(8), 1698-1709. 11.Zhang, W.1, Fu, J., Liu, J.,Wang, H., Schulz, E., Solanki, M., Boehme, P., Gebbing, M., Bergmann, T., Doerner, J., Stewart, A. F. & Ehrhardt, A.*, High-throughput cloning and tagging of an engineered adenovirus-library empowers vector diversity for broad applications,Molecular Therapy, 2015, 23, S21. 12.Bian, X.1, Tang, B.1, Yu, Y., Tu, Q., Gross, F.,Wang, H., Li, A., Fu, J., Shen, Y., Li, Y., Stewart, A. F., Zhao, G., Ding, X.*, Muller, R.* & Zhang, Y.*, Heterologous production and yield improvement of epothilones inBurkholderialesstrain DSM 7029,ACS Chemical Biology, 2017, 12(7), 1805-1812. 13.Yin, J.1,Wang, H., Li, R., Ravichandran, V., Bian, X., Li, A., Tu, Q., Stewart, A.F., Fu, J.* & Zhang, Y.*, A practical guide to recombineering inPhotorhabdusandXenorhabdus,Current Topics in Microbiology and Immunology, 2017. 14.Ravichandran, V.1, Zhong, L.,Wang, H., Yu, G., Zhang, Y.* & Li, A.*, Virtual screening and biomolecular interactions of CviR-based quorum sensing inhibitors againstchromobacterium violaceum,Front. Cell Infect. Microbiol., 2018, 8, 292. 15.Liu, Q.1, Shen, Q.1, Bian, X., Chen, H., Fu, J.,Wang, H., Lei, P., Guo, Z., Chen, W.*, Li, D.* & Zhang, Y.*, Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster inBacillus subtilisvia Red/ET recombineering,Scientific Reports, 2016, 6, 34623. 16.Jiang, B.1, Zhang, R., Feng, D., Wang, F., Liu, K., Jiang, Y., Niu, K., Yuan, Q., Wang, M.,Wang, H., Zhang, Y. & Fang, X.*, A Tet-on and Cre-loxP based genetic engineering system for convenient recycling of selection markers inPenicillium oxalicum,Frontiers in Microbiology, 2016, 7, 485. 17.Zhu, L.1, Yue, X., Han, K., Li, Z., Zheng, L., Yi, X.,Wang, H., Zhang, Y. & Li, Y.*, Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes inMyxococcus xanthus,Microbial Cell Factories, 2015, 14, 105. 18.Bian, X.1, Huang, F.,Wang, H., Klefisch, T., Muller, R.* & Zhang, Y.*, Heterologous production of glidobactins/luminmycins inEscherichia coliNissle containing the glidobactin biosynthetic gene cluster fromBurkholderiaDSM7029,Chembiochem, 2014, 15(15), 2221-2224. 19.Yu, F.1, Jing, X., Li, X.,Wang, H., Chen, H., Zhong, L., Yin, J., Pan, D., Yin, Y., Fu, J., Xia, L., Bian, X., Tu, Q. & Zhang, Y., Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency,Microbiol. Res., 2019, 218, 58-65.