个人简历

Professional appointments:\r
2019-present Principal Investigator/Associate Professor Department of Chemical Engineering, Tsinghua University, China\r
2016-2019 Principal Investigator/Assistant Professor Department of Chemical Engineering, Tsinghua University, China\r
2014-2016 Project Researcher Department of Chemistry and Biotechnology, The University of Tokyo, Japan\r
2010-2014 Postdoc Department of Chemical Engineering, Stanford University, USA\r
2009-2010 Postdoc Department of Biology, Johns Hopkins University, USA\r
\r
Education:\r
2004-2009 Ph.D. degree Department of Chemical Engineering, Tsinghua University, China\r
2000-2004 Bachelor's degree Department of Chemical Engineering, Tsinghua University, China

研究领域

卢元课题组研究的中心模式是利用工程学、生物学、化学、物理学、数学和计算机科学等多学科交叉手段操作生物大分子和复杂的生物网络。研究的焦点在于发展和应用世界最前沿的技术，突破天然生命体系的限制，快速改造生物分子机器、细胞和系统网络，以解决生物制造、人类健康等领域最具挑战性的工程难题。""

近期论文

2023\r
\r
Ting Wang#, Xiaomei Lin#, Yuting Li, Yuan Lu*. Artificial lipid biomembranes for full-length SARS-CoV-2 receptor. Advanced Materials, 2023: https://doi.org/10.1002/adma.202300575.\r
\r
Chen Wang#, Junzhu Yang#, Yuan Lu*. Click chemistry as a connection tool: Grand opportunities and challenges. Chinese Journal of Catalysis, 2023, In press.\r
\r
Xiaowen Cheng#, Chen Wang#, Dong Liu#, Yuting Liao, Bin Wang, Xue Zhang, Hao Zheng, Yuan Lu*. Dynamic artificial cells by swarm nanorobotics and synthetic life chemistry. SCIENCE CHINA Materials, 2023: https://doi.org/10.1007/s40843-022-2305-1.\r
\r
Qingyan Yuan, Minhui Wu, Yibo Liao, Shuli Liang, Yuan Lu*, Ying Lin*. Rapid prototyping enzyme homologs to improve titer of nicotinamide mononucleotide using a strategy combining cell-free protein synthesis with split GFP. Biotechnology and Bioengineering, 2023, 120(4): 1133-1146.\r
\r
Xihao Liao#, Changge Guan#, Cheng-Hua Wang#, Xinjie Chen#, Xiaomei Lin, Lianghua Lu, Lu Li, Ting Wang, Nan Su, Bian Wu, Xinquan Wang, Chong Zhang, Yi Wang*, Yuan Lu*, Xin-Hui Xing*. Cross-species binding spectrum of ACE2 to SARS-CoV-2 RBD. SCIENCE CHINA Life Sciences, 2023, 66(3): 639-642.\r
\r
Chuanting Qin#, Yi Wang#, Jiawang Hu, Ting Wang, Dong Liu, Jian Dong, Yuan Lu*. Artificial olfactory biohybrid system: An evolving sense of smell. Advanced Science, 2023, 10: 2204726.\r
\r
Dong Liu#, Dayin Sun#, Jiaxin Zhou#, Haoran Liu, Ruirui Guo, Bin Wang, Wenjun Ma, Zhenzhong Yang*, Yuan Lu*. Bionic morphological design and interface-free fabrication of halfmoon microrobots with enhanced motion performance. Chemical Engineering Journal, 2023, 452(Part 3): 139464.\r
\r
Dong Liu#, Ruirui Guo#, Shuangshuang Mao#, Yanjie Huang, Bin Wang, Zijian Wu, Xuanjie Xia, Jian Dong, Yu Xin, Ruiyang Xie, Jianzhong Shou, Wei Sun, Yuan Pang*, Yuan Lu*. 3D magnetic field guided sunflower-like nanocatalytic active swarm targeting patients-derived organoids. Nano Research, 2023: 16(1): 1021-1032.\r
\r
Yuting Liao#, Dong Liu#, Bin Wang, Zijian Wu, Miao Ni, Mengchen Wang, Cheng-Hua Wang*, Yuan Lu*. Photothermally-modulated magnetic nanochains as swarm nanorobotics for microreaction control. ACS Applied Nano Materials, 2023, 6(1): 21-33. (Cover)\r
\r
Dong Liu#, Yingying Liu#, Hua-Zhen Duan, Xinjie Chen, Yanan Wang, Ting Wang, Qing Yu, Yong-Xiang Chen*, Yuan Lu*. Customized synthesis of phosphoprotein bearing phosphoserine or its nonhydrolyzable analog. Synthetic and Systems Biotechnology, 2023, 8(1): 69-78.\r
\r
2022\r
\r
Bin Wang#, Dong Liu#, Yuting Liao, Yanjie Huang, Miao Ni, Mengchen Wang, Zhanpeng Ma, Zijian Wu, Yuan Lu*. Spatiotemporally actuated hydrogel by magnetic swarm nanorobotics. ACS Nano, 2022, 16(12): 20985-21001.\r
\r
Dong Liu, Ting Zhang, Yijia Guo, Yuting Liao, Zijian Wu, Hao Jiang, Yuan Lu*. Biohybrid magnetic microrobots for tumor assassination and active tissue regeneration. ACS Applied Bio Materials, 2022, 5(12): 5933-5942.\r
\r
Yi Cui#, Xinjie Chen, Ze Wang*, Yuan Lu*. Ribosome purification from Escherichia coli by ultracentrifugation. Biotechnology Notes, 2022, 3: 118-123.\r
\r
Xiaomei Lin#, Ting Wang#, Yuan Lu*. Cell-free synthetic biology: Orchestrating the machinery for biomolecular engineering. Biotechnology Notes, 2022, 3: 97-101.\r
\r
Junzhu Yang#, Cheng-Yen Lin#, Yi Feng#, Chen Wang, Jun Ge*, Yuan Lu*. Chemical-triggered artificial cell based on metal-organic framework. Chemical Engineering Journal, 2022, 450(Part 4): 138480.\r
\r
Dong Liu#, Ruirui Guo#, Bin Wang, Jiawang Hu, Yuan Lu*. Magnetic micro/nanorobots: A new age in biomedicines. Advanced Intelligent Systems, 2022: 2200208.\r
\r
Dong Liu#, Yanjie Huang#, Jiawang Hu, Bin Wang, Yuan Lu*. Multiscale catalysis under magnetic fields: Methodologies, advances, and trends. ChemCatChem, 2022: e202200889.\r
\r
Yanan Wang#, Xinjie Chen#, Wei Gao#, Lianju Ma*, Yuan Lu*. Mechanism investigation for efficient cell-free unnatural amino acid embedding. Process Biochemistry, 2022, 122 (Part 1): 306-314.\r
\r
Yi Cui#, Xinjie Chen#, Ze Wang, Yuan Lu*. Cell-free PURE system: Evolution and achievements. BioDesign Research, 2022: Article ID 9847014.\r
\r
Dong He#, Wen Zeng#, Yi Wang, Yifan Xing, Kang Xiong, Nan Su, Chong Zhang, Yuan Lu, Xin-Hui Xing*. Isolation and characterization of novel peptides from fermented products of Lactobacillus for ulcerative colitis prevention and treatment. Food Science and Human Wellness, 2022, 11(6): 1464-1474.\r
\r
Jiaqi Hou, Nan Jiang, Lianju Ma, Yuan Lu*. Cell-free protein synthesis: From basic research to engineering applications. Synthetic Biology Journal, 2022, 3(3): 465-486.\r
\r
Qingning Ye#, Xiaomei Lin#, Ting Wang#, Yuntao Cui, Hao Jiang*, Yuan Lu*. Programmable protein topology via SpyCatcher-SpyTag chemistry in one-pot cell-free expression system. Protein Science, 2022: 31(6):e4335.\r
\r
Xiaomei Lin#, Caijin Zhou#, Ting Wang#, Xiaoting Huang, Junxin Chen, Zhixia Li, Jisong Zhang*, Yuan Lu*. CO2-elevated cell-free protein synthesis. Synthetic and Systems Biotechnology, 2022: 7(3): 911-917.\r
\r
Ting Wang, Yuan Lu*. Advances, challenges and future trends of cell-free transcription-translation biosensors. Biosensors, 2022: 12(5): 318.\r
\r
Dong Liu, Ting Zhang, Xiaowen Cheng, Bin Wang, Yijia Guo, Zhengzuo Liu, Hao Jiang, Yuan Lu*. Engineering pollen-derived microstructures to reveal material morpho-performance paradigm. Small, 2022: 2200037.\r
\r
Ruirui Guo#, Dong Liu#, Yanjie Huang, Bin Wang, Jian Dong*, Yuan Lu*. Honeycomb-like active microswarms for magnetically tunable cascade enzyme catalysis. Nanoscale, 2022, 14(17): 6535-6542.\r
\r
Yanjie Huang#, Dong Liu#, Ruirui Guo, Bin Wang, Zhengzuo Liu, Yijia Guo, Jian Dong*, Yuan Lu*. Magnetic-controlled dandelion-like nanocatalytic swarm for targeted biofilm elimination. Nanoscale, 2022, 14(17): 6497-6506.\r
\r
Yuan Lu*. Textile-embedded cell-free biosensors. Nature Biomedical Engineering, 2022, 6: 225-226.\r
\r
Junzhu Yang#, Chen Wang#, Yuan Lu*. A temperature-controlled cell-free expression system by dynamic repressor. ACS Synthetic Biology, 2022, 11: 1408-1416. (Cover Article)\r
\r
Zhixia Li#, Yuting Li#, Xiaomei Lin#, Yuntao Cui, Ting Wang, Jian Dong*, Yuan Lu*. Supramolecular protein assembly in cell-free protein synthesis system. Bioresources and Bioprocessing, 2022, 9: 28.\r
\r
Shangsong Li#, Dong Liu#, Yuping Hu#, Zhijie Su, Xinai Zhang, Ruirui Guo, Dan Li, Yuan Lu*. Soft magnetic microrobot doped with porous silica for stability-enhanced multimodal locomotion in a nonideal environment. ACS Applied Materials & Interfaces, 2022, 14(8): 10856-10874.\r
\r
Dong Liu#, Ting Wang#, Yuan Lu*. Untethered microrobots for active drug delivery: From rational design to clinical settings. Advanced Healthcare Materials, 2022, 2102253.\r
\r
Jiaqi Hou#, Xinjie Chen#, Nan Jiang#, Yanan Wang, Yi Cui, Lianju Ma*, Ying Lin*, Yuan Lu*. Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system. Synthetic and Systems Biotechnology, 2022, 7(1): 522-532.\r
\r
2021\r
\r
Wenjun Ma, Dong Liu, Sida Ling, Jingwei Zhang, Zhuo Chen*, Yuan Lu, Jianhong Xu*. High-throughput and controllable fabrication of helical microfibers by hydrodynamically focusing flow. ACS Applied Materials & Interfaces, 2021, 13(49): 59392–59399.\r
\r
Xinjie Chen, Yuan Lu*. Circular RNA: Biosynthesis in vitro. Frontiers in Bioengineering and Biotechnology, 2021, Article 787881.\r
\r
Chen Wang#, Junzhu Yang#, Yuan Lu*. Modularize and unite: Toward creating a functional arti?cial cell. Frontiers in Molecular Biosciences, 2021, Article 781986.\r
\r
Jian Li*, Yong-Chan Kwon*, Yuan Lu*, Simon J. Moore*. Editorial: Cell-free synthetic biology. Frontiers in Bioengineering and Biotechnology, 2021, Article 799122.\r
\r
Dong Liu#, Shangsong Li#, Ting Zhang, Hao Jiang, Yuan Lu*. 3D magnetic field-controlled synthesis, collective motion, and bioreaction enhancement of multifunctional peasecod-like nanochains. ACS Applied Materials & Interfaces, 2021, 13: 36157-36170.\r
\r
Junzhu Yang#, Liyuan Zhang#, Cheng Zhang#, Yuan Lu*. Exploration on the expression and assembly of virus-like particles. Biotechnology Notes, 2021, 2: 51-58.\r
\r
Junzhu Yang#, Yuntao Cui#, Zhou Cao#, Shengnan Ma, Yuan Lu*. Strategy exploration for developing robust lyophilized cell-free systems. Biotechnology Notes, 2021, 2: 44-50.\r
\r
Xinjie Chen#, Yingying Liu#, Jiaqi Hou#, Yuan Lu*. A linear DNA template-based framework for site-specific unnatural amino acid incorporation. Synthetic and Systems Biotechnology, 2021, 6: 192-199.\r
\r
Du-Xin Liu#, Jiali Bao#, Dong Liu#, Yuan Lu, Jing Xu*. Modeling of planar hydraulically amplified self-healing electrostatic actuators. IEEE Robotics and Automation Letters, 2021, 6(4): 7533-7540.\r
\r
Yifan Xing#, Junyu Liu#, Xiaojie Guo, Haipeng Liu, Wen Zeng, Yi Wang, Chong Zhang, Yuan Lu, Dong He, Shaohua Ma, Yonghong He, Xin-Hui Xing*. Engineering organoid microfluidic system for biomedical and health engineering: A review. Chinese Journal of Chemical Engineering, 2021, 30: 244-254.\r
\r
Liyuan Zhang#, Xiaomei Lin#, Ting Wang#, Wei Guo*, Yuan Lu*. Development and comparison of cell-free protein synthesis systems derived from typical bacterial chassis. Bioresources and Bioprocessing, 2021, 8: 58.\r
\r
Cheng-Yen Lin#, Yi Feng#, Jun Ge*, Yuan Lu*. Impact of metal–organic frameworks on protein expression. Chemical Research in Toxicology, 2021, 34: 1403-1408.\r
\r
Xinjie Chen, Yuan Lu*. In silico design of linear DNA for robust cell-free gene expression. Frontiers in Bioengineering and Biotechnology, 2021, Article 670341.\r
\r
Xiaomei Lin, Zhixia Li, Yuting Li, Yuan Lu*. A robust Escherichia coli cell-free expression toolbox driven by sigma factors. Biochemical Engineering Journal, 2021, 171: 108031.\r
\r
Nan Jiang#, Xuanwei Ding#, Yuan Lu*. Development of a robust Escherichia coli-based cell-free protein synthesis application platform. Biochemical Engineering Journal, 2021, 165: 107830.\r
\r
Jun-Yu Liu, Dong He, Yi-Fan Xing, Wen Zeng, Kai Ren, Chong Zhang, Yuan Lu, Song Yang, Shu-Jian Ou, Yi Wang*, Xin-Hui Xing*. Effects of bioactive components of Pu-erh tea on gut microbiomes and health: A review. Food Chemistry, 2021, 353: 129439.\r
\r
2020\r
\r
Chen Wang#, Yuhao Geng#, Qi Sun#, Jianhong Xu*, Yuan Lu*. A sustainable and efficient artificial microgel system: Toward creating a configurable synthetic cell. Small, 2020: 2002313.\r
\r
Junzhu Yang, Yuan Lu*. Physical stimuli-responsive cell-free protein synthesis. Synthetic and Systems Biotechnology, 2020, 5(4):363-368.\r
\r
Eunhee Cho, Yuan Lu*. Compartmentalizing cell-free systems: Toward creating life-like artificial cells and beyond. ACS Synthetic Biology, 2020, 9(11): 2881–2901. (Front Cover Article)\r
\r
Xiaomei Lin, Yuting Li, Zhixia Li, Rui Hua, Yuyang Xing, Yuan Lu*. Portable environment-signal detection biosensors with cell-free synthetic biosystems. RSC Advances, 2020, 10: 39261-39265.\r
\r
Peng Zhang#, Junzhu Yang#, Eunhee Cho#, Yuan Lu*. Bringing light into cell-free expression. ACS Synthetic Biology, 2020, 9: 2144-2153. (Cover Article)\r
\r
Liyuan Zhang, Wei Guo*, Yuan Lu*. Advances in cell-free biosensors: Principle, mechanism, and applications. Biotechnology Journal, 2020, 2000187(Front Cover Article)\r
\r
Nan Jiang, Lianju Ma*, Yuan Lu*. Cell-free synthetic biology in the new era of enzyme engineering. Chinese Journal of Chemical Engineering, 2020, 28(11):2810-2816.\r
\r
Dong Liu, Zhenghuan Yang, Luyang Zhang, Minglun Wei, Yuan Lu*. Cell-free biology using remote-controlled digital micro?uidics for individual droplet control. RSC Advances, 2020, 10: 26972-26981\r
\r
Xinjie Chen#, Qi Sun#, Yuan Lu*. Creating a locally crowded environment with nanoclay hydrogels for cell-free biosynthesis. Soft Matter, 2020, 16: 5132-5138 (Cover Article)\r
\r
Xiaomei Lin#, Caijin Zhou#, Jisong Zhang*, Yuan Lu*. O2-tuned protein synthesis machinery in Escherichia coli-based cell-free system. Frontiers in Bioengineering and Biotechnology, 2020, 8: Article 312\r
\r
Caijin Zhou#, Xiaomei Lin#, Yuan Lu*, Jisong Zhang*. Flexible on-demand cell-free protein synthesis platform based on a tube-in-tube reactor. Reaction Chemistry & Engineering, 2020, 5: 270-277 (Front Cover Article)\r
\r
Yang Ji#, Yi Wang#, Wen Zeng, Xiang Mei, Shanshan Du, Yishu Yan, Jie Hao, Zhenqing Zhang, Yuan Lu, Chong Zhang, Jun Ge, Xin-Hui Xing*. A heparin derivatives library constructed by chemical modification and enzymatic depolymerization for exploitation of non-anticoagulant functions. Carbohydrate Polymers, 2020, 249:116824\r
\r
2019\r
\r
Licheng Wang, Hanyi Hou, Xinqi Jiao, Tianyin Shen, Yingying Liu, Yida Qiao, Yu Liu, Yuan Lu*, Jun Ge*. Bifunctional Therapy by Zinc-Cobalt Bimetal-Organic Framework with Encapsulated Doxorubicin to Overcome Drug-resistance.ChemNanoMat, 2019, 5: 1531-1539\r
\r
Yuan Lu, Jamin Koo*. O2 sensitivity and H2 production activity of hydrogenases-A review. Biotechnology and Bioengineering, 2019,116:3124-3135\r
\r
Yingying Liu, Ning Bu*, Yuan Lu*. Cell-free synthetic biology: an emerging strategy to revolutionize the biomedical industry. Chinese Journal of Biotechnology, 2019, 35(12): 2269-2283\r
\r
Peng Zhang, Huibao Feng, Junzhu Yang, Hao Jiang*, Hongjun Zhou, Yuan Lu*. Detection of inorganic ions and organic molecules with cell-free biosensing systems. Journal of Biotechnology, 2019, 300: 78-86\r
\r
Peng Zhang, Junqian Wang, Xuanwei Ding, Jun Lin, Hao Jiang*, Hongjun Zhou, Yuan Lu*. Exploration of the tolerance ability of a cell-free biosynthesis system to toxic substances. Applied Biochemistry and Biotechnology, 2019, 189:1096-1107\r
\r
Yi-Fan Xing, Dong He, Yi Wang, Wen Zeng, Chong Zhang, Yuan Lu, Nan Su, Yan-Hua Kong, Xin-Hui Xing. Chemical constituents, biological functions and pharmacological effects for comprehensive utilization of Eucommia ulmoides Oliver. Food Science and Human Wellness, 2019, 8: 177-188\r
\r
Wei Gao#, Eunhee Cho#, Yingying Liu#, Yuan Lu*. Advances and challenges in cell-free incorporation of unnatural amino acids into proteins. Frontiers in Pharmacology, 2019, 10, Article 611\r
\r
Wei Gao, Ning Bu*, Yuan Lu*. Efficient incorporation of unnatural amino acids into proteins with a robust cell-free system. Methods and Protocols, 2019, 2(1):16\r
\r
2018\r
\r
Yishu Yan#, Yuan Lu#, Xiaoni Liu, Yan Zhang*, Jinghua Chen*. pH-driven precise control of hybridization reaction kinetics for rapid DNA assay. ChemistrySelect, 2018, 3(38):10646-10650\r
\r
Wei Gao, Ning Bu*, Yuan Lu*. Recent advances in cell-free unnatural protein synthesis. Chinese Journal of Biotechnology, 2018, 34(9): 1371?1385\r
\r
Xuanwei Ding#, Dong Liu#, George Booth, Wei Gao, Yuan Lu*. Virus-like particle engineering: From rational design to versatile applications. Biotechnology Journal, 2018, 13(5):1700324 (Front Cover Article)\r
\r
Yang Ji, Yuan Lu, Yishu Yan, Xinxin Liu, Nan Su, Chong Zhang, Shengli Bi, Xin‐Hui Xing. Design of fusion proteins for efficient and soluble production of immunogenic Ebola virus glycoprotein in Escherichia coli. Biotechnology Journal, 2018:1700627\r
\r
Dong He, Wenming Zhu, Wen Zeng, Jun Lin, Yang Ji, Yi Wang, Chong Zhang, Yuan Lu, Daoquan Zhao, Nan Su, Xin-Hui Xing. Nutritional and medicinal characteristics of Chinese giant salamander (Andrias davidianus) for applications in healthcare industry by artificial cultivation: A review. Food Science and Human Wellness, 2018, 7(1):1-10 (Front Cover Article)\r
\r
Peng Zhang#, Jun Lin#, Yuan Lu*. Advances in cell-free protein synthesis. Chinese Journal of Bioprocess Engineering, 2018, 16(01): 59-66\r
\r
Zhidan Liu, Buchun Si, Jiaming Li, Jianwei He, Chong Zhang, Yuan Lu, Yuanhui Zhang, Xin-Hui Xing. Bioprocess engineering for biohythane production from low-grade waste biomass: technical challenges towards scale up. Current Opinion in Biotechnology, 2018, 50: 25-31