个人简历

教育经历\r
博士后 2008-2015 美国Purdue大学 物理系、化学系、生物医学工程系\r
博士 2002-2007 中国科学院武汉物理与数学研究所毕业\r
学士 1998-2002 武汉大学理学院物理系毕业\r
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工作经历\r
2015.3-至今 教授 华中科技大学光电国家实验室Britton Chance生物医学光子学研究部。\r
2008.3-2015.2 博士后 美国Purdue大学物理系、化学系、生物医学工程系

研究领域

""基因靶向的多色蛋白质Raman标签\r
细胞是组成生命的最基本单元。虽然细胞的尺寸只有微米大小，但其组成结构和代谢活动高度复杂且精确调控。由于成像方法和技术的限制，细胞对于目前的人类来说，仍然是一个黑匣子，我们无法在活体上实时观察成千上万种蛋白的分布和活动。荧光标记由于光谱线宽太宽，只能同时报告几种蛋白质在细胞内的位置和分布。我们实验室从一个独特而交叉的研究方向出发，发展基于非天然氨基酸的分子生物学Raman标记策略，希望实现基因靶向，且分子量和分子尺寸更小，光谱线宽更窄，标记数量更多的蛋白标记方法。\r
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超分辨无标记成像\r
恩斯特-阿贝(Ernst Abbe)在150年前发现的光学分辨率理论极限，长期以来一直被认为是不可能被打破的。21 世纪初，突破光学衍射极限的超分辨成像帮助生物学家们对活体细胞的观测能力从200纳米级分辨率跨越到了20纳米水平。超分辨光学成像包括STORM,STED,SIM和PALM都依赖荧光标记技术才可实现，然而很多组织和生物组分是无法标记的。我们主要突破无标记的Raman超分辨成像技术，对细胞内结构和代谢分子进行无标记的光学超分辨成像。目前我们已经将无标记超分辨SRS成像的分辨率提高到了110 纳米，后期我们将对临床样品进行超分辨成像研究。\r
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超快的化学反应成像\r
超快的化学反应相干Raman成像：化学反应，因其发生的过程在毫秒甚至微秒量级，反应时间极短，因此目前没有任何形式的显微成像技术能够对剧烈的化学反应进行亚微米和亚毫秒时空分辨的化学成像和监测。我们发展出Collinear Multiple Beam based Stimulated Raman Scattering Microscope (COMB-SRS)成像系统，能够在亚微米空间分辨下实现2000 Hz的相干Raman化学成像。该超高速的分子成像显微镜可以跟上剧烈的高分子聚合反应速度，帮助我们量化测量自由基触发的水凝胶分子聚合反应动力学过程。在化学分子成像过程中，我们首次观察到了光引发的聚合反应中聚合波的存在。\r
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超光谱受激Raman分子成像\r
传统生物学显微成像技术一直依赖荧光标记获取组织中细胞图像的对比度。为实现无标记的显微成像，我们发展了超光谱受激拉曼分子成像技术(Hyperspectral Stimulated Raman Scattering microscopy)，对鼠脑皮层神经网络以及神经活动进行免标记成像与测量;追踪单细胞内重要生物分子、代谢物、特定蛋白在空间的组织分布；对临床癌症组织内的脂质代谢进行免标记的成像研究，寻找癌症中代谢异常的生物分子标志物。""""

近期论文

Wei Zhu#, Er-Li Cai#, Hao-Zheng Li, Ping Wang*, Ai-Guo Shen*, Jürgen Popp*, Ji-Ming Hu*. Precise Encoding of Triple-Bond Raman Scattering of Single Polymer Nanoparticles for Multiplexed Imaging Application. Angew Chem Int Ed Engl. 60(40):21846-21852.(2021) (Impact factor:15.336)\r
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Yingxue Ma, Haozheng Li, Zhou Gong, Shuai Yang, Ping Wang*, and Chun Tang*.Nucleobase Clustering Contributes to the Formation and Hollowing of Repeat-Expansion RNA Condensate.Journal of American Chemical Society (10.1021/jacs.1c12085 ) (Impact factor:15.419)\r
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Guang Yang#, Chi Yang#, Yage Chen, Boyu Yu, Yali Bi, Jiangshan Liao, Haozheng Li, Hong Wang, Yuxi Wang, Ziyu Liu, Zongsog Gan, Quan Yuan, Yi Wang*, Jinsong Xia*, and Ping Wang*. Direct Imaging of Integrated Circuits in CPU with 60 nm Super-Resolution Optical Microscope. Nano Letters 9,3887-3893(2021) (Impact factor:11.189)\r
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Chi Yang#, Yali Bi#, Yage Chen, Songlin Huang, Zhihong Zhang, and Ping Wang*. Pulse-sheet chemical tomography by counterpropagating stimulated Raman scattering. Optica 8,3,396-401(2021) (Impact factor:11.104)\r
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Yali Bi#, Chi Yang#, Lei Tong#, Haozheng Li, Boyu Yu, Shuai Yan, Guang Yang, Meng Deng, Yi Wang, Wei Bao*, Lei Ye*, Ping Wang*. Far-field transient absorption nanoscopy with sub-50 nm optical super-resolution. Optica 7,10 (2020) (Impact factor:11.104)\r
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H. Li, Y. Cheng, H. Tang, Y. Bi, Y. Chen, G. Yang, S. Guo, S. Tian, J. Liao, X. Lv, S. Zeng, M. Zhu, C. Xu, J. Cheng*,P. Wang*. Imaging Chemical Kinetics of Radical Polymerization with an Ultrafast Raman Microscope. Advanced Science 7, 1903644 (2020) (Impact factor:16.806) \r
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S. Tian, et al, F. Meng*, J. Lauher*, P. Wang* & Liang Luo*. Polydiacetylene-based Ultrastrong Bioorthogonal Raman Probes for Targeted Live-cell Raman Imaging. Nature Communications 11, 81 (2020) (Impact factor:14.919) \r
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Y. Chen#, S. Liu, J#, et al, D. Zhu*, K. Wang*, P. Wang*. Coherent Raman Scattering Unravelling Mechanisms Underlying Skull Optical Clearing for Through-Skull Brain Imaging. Analytical Chemistry 15, 9371 (2019) (Impact factor:6.986) \r
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J. Zhang#, S. Yan#, Z. He, C. Ding, T. Zhai, Y. Chen, H. Li, G. Yang, X. Zhou, P. Wang*. Small Unnatural Amino Acid Carried Raman Tag for Molecular Imaging of Genetically Targeted Proteins. The journal of physical chemistry letters 9, 4679-4685 (2018) (Impact factor:6.475) \r
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Y. Bi#, C. Yang#, Y. Chen, S. Yan, G. Yang, Y. Wu, G. Zhang*, P. Wang*. Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm. Light: science & applications 7, 81 (2018) (Impact factor:17.782) \r
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S. Yan#, S. S. Cui#, K. Ke, B. X. Zhao, X. L. Liu, S. H. Yue*, P. Wang*. Hyperspectral Stimulated Raman Scattering Microscopy Unravels Aberrant Accumulation of Saturated Fat in Human Liver Cancer. Anal. Chem. 90, 6362-6366 (2018) (Impact fdfactor:6.986) \r
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B. Huang#, S. Yan#, L. Xiao, R. Ji, L. Yang, A. J. Miao*, P. Wang*. Label-Free Imaging of Nanoparticle Uptake Competition in Single Cells by Hyperspectral Stimulated Raman Scattering. Small 14, (2018) (Impact factor:13.281) \r
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Y. Rong#, X. Hou#, Y. Hu, A. Mei, L. Liu, P. Wang, H. Han*. Synergy of Ammonium Chloride and Moisture on Perovskite Crystallization for Efficient Printable Mesoscopic Solar Cells. Nature communications 8, 14555 (2017) (Impact factor:14.919) \r
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C. S. Liao#, M. N. Slipchenko#, P. Wang#, J. Li, S. Y. Lee, R. A. Oglesbee, J. X. Cheng*. Microsecond Scale Vibrational Spectroscopic Imaging by Multiplex Stimulated Raman Scattering Microscopy. Light: science & applications 4, (2015) (Impact factor:17.782) \r
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B. Liu#, P. Wang#, J. I. Kim, D. L. Zhang, Y. Q. Xia, C. Chapple*, J. X. Cheng*. Vibrational Fingerprint Mapping Reveals Spatial Distribution of Functional Groups of Lignin in Plant Cell Wall. Anal. Chem. 87, 9436-9442 (2015) (Impact factor:6.986) \r
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C. S. Liao*, P. Wang*, P. Wang, J. J. Li，H. J. Lee, G. Eakins, J. X. Cheng. Spectrometer-free vibrational imaging by retrieving stimulated Raman signal from highly scattered photons. Science Advances 1, (2015) (Impact factor:14.143)