个人简历

华南师范大学生物光子学研究院院长、学术委员会主任，激光生命科学教育部重点实验室教授，博士生导师，广东省“千百十人才工程”省级培养对象。主持国家863项目1项（230万）、国家重大科研仪器研制项目1项（510万）、国家自然科学基金面上项目5项，主持广东科技计划项目1项、重点项目1项以及其他省部级项目4项。研究领域主要涉及生物医学光子学、生物物理学、肿瘤细胞生物学、分子生物学。发表学术论文140余篇，获得授权国家发明专利5项。
教育简历：
1982.9-1986.6：湖北师范学院物理专业毕业，获理学学士学位；
1990.9-1993.5：华中科技大学光电子与物理电子专业毕业，获理学硕士学位；
1999.9-2003.5：华中科技大学生物医学工程专业毕业，获工学博士学位；
工作简历：
1986.6-1990.9：湖北师范学院物理系教师，助教；
1993.9-2000.9：湖北师范学院物理系教师，讲师；
2000.9-2003.11：华中科技大学生命科学院，副教授；
2003.11-2005.12：华南师范大学，副教授, 副所长；
2005.12-2013: 华南师范大学激光生命科学教育部重点实验室 教授，博士导师；
2013-2018.06: 华南师范大学生物光子学研究院学术委员会主任，教授，博导；
2018.07-至今: 华南师范大学生物光子学研究院院长、学术委员会主任，教授，博导；
主持承担的主要基金项目
1. 国家重大科研仪器研制项目（主持）：定量FRET显微成像仪的研制(61527825)，研究总经费510.32万元，直接经费442.1万元，起止年月：2016.01-2020.12
2. 国家自然科学基金面上项目（主持）：基于线性光谱分离的活细胞实时定量FRET显微成像技术研究(81471699) 80万，起止年月：2015.01-2018.12
3. 广东省科技计划项目（主持）：活细胞FRET光谱定量检测仪的研制(2014B090901060)，100万,起止年月：2015.01-2018.12
4. 广州市科技计划项目（主持）：光谱FRET定量检测分析技术研究（2014J4100055）, 8万元，起止年月：2014.04-2016.04
5. 国家自然科学基金面上项目（主持）：活细胞中蛋白质寡聚化动态过程实时荧光成像与定量分析技术研究(61178078)，78万元，起止年月：2012.1-2015.12.
6. 国家自然科学基金面上项目（主持）：显微光学成像术研究紫杉醇诱导肿瘤细胞内质网空泡化和类副凋亡的分子调控机理(31071218)，35万元，起止年月：2011.1-2013.12.
7. 广东省高教厅、财政厅重点项目（主持）：非小细胞肺癌对吉非替尼耐药分子机制的单细胞可视化研究(cxzd115)，25万元，起止年月：2011.10-2014.10。
8. 国家自然科学基金面上项目（主持）：荧光技术在活细胞中实时研究中药诱导肿瘤细胞凋亡的分子机理(30670507)，28万元，起止年月：2007.1-2009.12。
主要获奖
（1） 组织光学成像理论若干关键问题研究，2003年度湖北省自然科学二等奖，骆清铭，曾绍群，朱丹，吴龟灵，陈同生。
（2） 光学与生命科学交叉科学中的前沿问题与关键技术研究.2009年度广东省科学技术奖（自然科学类）二等奖，邢达，朱德斌，陈同生，章春笋 张玲瑞，刘镭，唐永红等。

研究领域

生物医学光子学、细胞生物学""

近期论文

1 Hai-yang Li, Cai-ping Zhuang, Xiao-ping Wang,Tong-sheng Chen*. SB203580 enhances the RV-induced loss of mitochondrial membrane potential and apoptosis in A549 cells. Proc. of SPIE 2012, 8224, 82240L(EI收录)
2 Chao-yang Wang,Tong-sheng Chen*.Taxol-induced paraptosis-like A549 cell death is not senescence. Proc. of SPIE 2011, 7900: 79000K-1（EI收录）
3 Huai-na Yu, Ying-ying Lu,Tong-sheng Chen*. Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells. Proc. of SPIE 2011, 7900: 79000J-1（EI收录）
4 Wen-jing Guo,Tong-sheng Chen*. Taxol induces concentration-dependent phosphatidylserine (PS) externalization and cell cycle arrest in ASTC-a-1 cells. Proceedings of the SPIE, 7565：75650J-75650J-7 (2010). DOI: 10.1117/12.839552 （EI收录）
5 Feng-lian Xiao,Tong-sheng Chen*, Jun-Le Qu, Cheng-Yi Liu. Artemisinin induces ROS-mediated caspase3 activation in ASTC-a-1 cells. Proceedings of the SPIE, Volume 7565, pp. 75650I-75650I-7 (2010)，DOI: 10.1117/12.839551 (EI收录）
6 Wei-wei Zhang, Zhi-ping Wang,Tong-sheng Chen*.Bax is not involved in the resveratrol-induced apoptosis in human lung adenocarcinoma cells. Proceedings of the SPIE, Volume 7565, pp. 75650K-75650K-7 (2010)，DOI: 10.1117/12.839631. (EI收录）
7 Ying-Ying Lu,Tong-Sheng Chen*，Jun-Le Qu. Bax translocation into mitochondria during dihydroartemisinin(DHA)-induced apoptosis in human lung adenocarcinoma cells. Proc. of SPIE 2009，7178, 71780J
8 Wen-liang Pan,Tong-sheng Chen*,Jun-Le Qu. Emission spectral analysis of caspase-3 activation during artesunate-induced apoptosis of human lung adenocarcinoma cell. Proc. of SPIE 2009，7178, 71780I
9 Qingrui Sun,Tongsheng Chen*.Reactive oxygen species (ROS) is not a promotor of taxol-induced cytoplasmic vacuolization. Proc. of SPIE 2009，7178, 71780K
10 Tong-sheng Chen *, Lei Sun, Huiying Wang, Longxiang Wang．Fluorescence Imaging Analysis of Taxol-Induced Cell Death with Cell Swelling and Cytoplasmic Vacuolization．Proceedings of the SPIE 2008, 6857, 68570L-68570L-8 (2008) (EI收录)．
11 Lei Sun,Tongsheng Chen*, Longxiang Wang, Huiying Wang．Analysis of caspase3 activation in ChanSu-induced Apoptosis of ASTC-a-1 Cells by Fluorescence Techniques．Proceedings of the SPIE 2008, 6857, 68570I-68570I-8 (2008) (EI收录)．
12 Huiying Wang,Tongsheng Chen*,Lei Sun．Analysis of caspase-3 activation in mitomycin C-induced ASTC-a-1 cell apoptosis using acceptor photobleaching techniques．Proceedings of the SPIE 2008, 6857, pp. 68570J-68570J-7 (2008) (EI收录)．
13 Tongsheng Chen, et al. Dynamic imaging of interaction between proteins 14-3-3and Bid in living cells, SPIE, 2006, 6087:60870L (EI收录)
14 Tongsheng Chen, et al. Real-time single cell analysis of molecular mechanism of apoptosis and preliforaion using FRET technique, SPIE, 2006, 6047:604736 (EI收录)
15 Tongsheng Chen, Da Xing. Application of the FRET for Monitoring the Dynamics of Caspase-3 Activation during Apoptosis in Living Cells. SPIE, 2005, 5630: 368（EI收录）
16 Tongsheng Chen, Da Xing. Application of the FRET for Monitoring the Dynamics of Caspase-3 Activation during Apoptosis in Living Cells. SPIE, 2004, 5630:368 (EI收录)
17 Tongsheng Chen, Shaoqun Zeng, Qingming Luo et al., High-Order Photobleaching in Two-Photon Excitation FluorescenceMicroscopy Inside Live Cell. SPIE. 2003 . 4967: 63-67（EI收录）
18 Tongsheng Chen, Yafeng Liu, Shaoqun Zeng, Qingming Luo. Photobleaching of fluorophores at lower concentration under two-photon excitation microscopy. SPIE, 2003，5254:508-512（EI收录）
19 Tongsheng Chen,Shaoqun Zeng, Qingming Luo. Interaction way of photobleaching produced bythe interaction between excitation photons and fluorophores molecules in excited states. SPIE,2003，4967:185-190（EI收录）
20 Tongsheng Chen,et. al. Data analysis in green fluorescent protein based resonance energy transfer. SPIE. 2000, 4224: 346-350 (EI收录)。
1 Tan Li, Liping Wu, Jiang Zhang, Gaina Xi, Yilin Pang, Xiaoping Wang and Tongsheng Chen*. Hydrothermal Reduction of Polyethylenimine and Polyethylene Glycol Dual-Functionalized NanoGraphene Oxide for high-efficiency Gene Delivery. ACS Appl. Mater. Interfaces 2016, 8, 31311−31320.
2 Tan Li, Hongyu Liu, Gaina Xi, Yiling Pang, Liping Wu, Xiaoping Wang *, Tongsheng Chen*. One step reduction and PEIylation ofPEGylated nanographene oxide for high-efficient chemo-photothermal therapy. J Mater Chem B 2016, 4: 2972 – 2983.
3 Yingyao Quan, Guiqi Qin, Hao Huang, Yuhong Liu, Xiaoping Wang, Tongsheng Chen*. Dominant roles of Fenton reaction in sodium nitroprusside-induced chondrocyte apoptosis. Free Radical Biology & Medicine 2016, 94: 135-144.
4 Mengyan Du, Lili Zhang, Shusen Xie, Tongsheng Chen*. Wide-field microscopic FRET imaging using simultaneous spectral unmixing of excitation and emission spectra. Opt. Express 2016, 24(14), 16037-16051.
5 aina Xi, Xiaoping Wang, Tongsheng Chen*. A reduced graphene oxide-based FRET sensor for highly sensitive detection of matrix metalloproteinase 2. Int J Nanomed 2016, 11:1537–1547
6 Jiang Zhang, Fangrui Lin, Liuying Chai, Lichun Wei, Tongsheng Chen*. IIem-spFRET: Improved Iem-spFRET method for robust FRET measurement. J Biomed Opt 2016, 21(10), 105003
7 Yilin Pang, Guiqi Qin, Liping Wu, Xiaoping Wang, Tongsheng Chen*. Artesunate induces ROS-dependent apoptosis via a Bax-mediated intrinsic pathway in Huh-7 and Hep3B cells. Experimental Cell Research 2016, 347: 251–260
8 Hongyu Liu, Tan Li, Yuhong Liu, Guiqi Qin, Xiaoping Wang, and Tongsheng Chen*. Glucose-reduced Graphene Oxide with Excellent Biocompatibility and Photothermal Efficiency as well as Drug Loading. Nanoscale Res Lett 2016, 11:211.
9 Jiang Zhang, Lili Zhang, Liuying Chai, Fangfang Yang, Mengyan Du, Tongsheng Chen*. Reliable measurement of the FRET sensitized-quenching transition factor for FRET quantification in living cells. Micron 2016, 88:7-15.
10 ZP Wang, Y Li, Q Zhou, YF Wang, TS Chen. Berberine-loaded solid lipid nanoparticles enhance hypoglycemic efficacy on streptozotocin-induced diabetic C57BL/6 mice. Nanomed-Nanotechnol Biol Med 2016, 12(2): 483-484.
11 XL Li and TS Chen. Enhancement and suppression effects of a nanopatterned surface on bacterial adhesion. PHYSICAL REVIEW E 2016(93), 052419.
12 H Huang, YY Quan, XP Wang, TS Chen. Gold Nanoparticles of Diameter 13 nm Induce Apoptosis in Rabbit Articular Chondrocytes. Nanoscale Res Lett 2016, 11:249.
1 Lili Zhang, Guiqi Qin, Liuying Chai, Jiang Zhang, Fangfang Yang, Hongqin Yang, Shusen Xie,Tongsheng Chen*. Spectral wide-field microscopic FRET imaging in live cells.J. Biomed. Opt. 2015,20(8), 086011
2 Guiqi Qin, Hongyu Liu, Liping Wu, Yilin Pang, Chubiao Zhao, Shengnan Wu,Xiaoping Wang,Tongsheng Chen*.Artesunate inducesapoptosis via a ROS-independentBax-mediated intrinsic pathway in HepG2 cells. Exp Cell Res 2015, 336(2):308-317
3 Guiqi Qin, Lili Zhang, Hongyu Liu, Yingyao Quan, Liuying Chai, Shengnan Wu, Xiaoping Wang,Tongsheng Chen*. Dihydroartemisinin induces apoptosis preferentially via a Bim/Bak-mediated intrinsic pathway in hepatocarcinoma cells. Apoptosis 2015, 20:1072–1086.
4 Liuying Chai, Jianwei Zhang, Lili Zhang,Tongsheng Chen*.Miniature fiber optic spectrometer-based quantitative fluorescence resonance energy transfer measurement in single living cells,\