个人简历

李大力，博士，2007年获湖南师范大学遗传学博士学位，博士期间在美国德州农工大学进行联合研究,毕业后受聘于华东师范大学生命科学学院从事教学与科研工作，2009和2014 年先后破格晋升为副教授、研究员，2016年获得教育部奖励计划青年项目资助。目前为华东师范大学实验动物中心主任，上海市调控生物学重点实验室副主任。近年来在国际知名生物学期刊Nature Biotechnology，Nucleic Acids Research和Cell Research等杂志上发表研究论文60余篇，在国际上率先建立了大鼠和小鼠基因编辑技术体系，并成功利用CRISPR/Cas9技术在动物模型中治愈遗传疾病。主持国家自然科学基金4项，上海市项目2项。Scientific Reports杂志编委，为Nature Biotechnology等国际知名期刊审稿。

研究领域

1.基因编辑技术体系的优化与遗传疾病的基因治疗研究 2.哺乳动物模型的遗传密码扩充技术 3.成体干细胞自我更新与分化的机理及应用研究

近期论文

Li D*, Qiu Z, Shao Y, Chen Y, Guan Y, Liu M, Li Y, Gao N, Wang L, Lu X, Zhao Y*, Liu M*. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nature Biotechnology. 2013 ;31(8):681-3 Guan Y, Ma L, Li Q, Sun Z, Ma Li, Wu L, Wang L, Zeng L, Shao Y, Chen Y, Ma N, Lu W, Hu K, Han H, Yu Y, Huang Y, Liu M*, Li D*. CRISPR/Cas9 mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse. EMBO Mol Med. 2016 May 2;8(5):477-88 News&Views, Nguyen TH, Anegon I. Successful correction of hemophilia by CRISPR/Cas9 genome editing in vivo: delivery vector and immune responses are the key to success.EMBO Mol Med. 2016 May 2;8(5):439-41.) （杂志同期点评） Chen Y, Ma J, Lu W, Tian M, Thauvin M, Yuan C, Volovitch M, Wang Q, Holst J, Liu M, Vriz S, Ye S*, Wang L*, Li D*. Heritable expansion of the genetic code in mouse and zebrafish. Cell Res. 2017 Feb;27(2):294-297. Editor’s Choice, Valda Vinson, Expanding the genetic code in vertebrates, Science 2017:Vol. 355, Issue 6320, pp. 36 Shao Y, Guan Y, Wang L, Qiu Z, Liu M, Chen Y, Wu L, Li Y, Ma X, Liu M*, Li D*. Genome editing in the rat using CRISPR-Cas and injection of RNAs into one-cell embryos. Nature Protocols. 2014 (10):2493-512 Qiu Z, Liu M, Chen Z, Shao Y, Pan H, Wei G, Yu C, Zhang L, Li X, Wang P, Fan HY, Du B, Liu B, Liu M*, Li D*. High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases. Nucleic Acids Res. 2013 Jun;41(11):e120 2017 Wen H, Ma H, Cai Q, Lin S, Lei X, He B, Wu S, Wang Z, Gao Y, Liu W, Liu W, Tao Q, Long Z, Yan M, Li D, Kelley KW, Yang Y, Huang H, Liu Q. Nat Med. 2018 Jan 1. doi: 10.1038/nm.4456. Yue Z, Yuan Z, Zeng L, Wang Y, Lai L, Li J, Sun P, Xue X, Qi J, Yang Z, Zheng Y, Fang Y, Li D, Siwko S, Li Y, Luo J, Liu M. FASEB J. 2017 Dec 21. Wei Y, Zhang XH, Li DL*. The 'new favorite' of gene editing technology-single base editors. Yi Chuan. 2017 Dec 20;39(12):1115-1121. （invited review） Peng W, Li M, Li H, Tang K, Zhuang J, Zhang J, Xiao J, Jiang H, Li D, Yu Y, Sham PC, Nattel S, Xu Y. Dysfunction of Myosin Light-Chain 4 (MYL4) Leads to Heritable Atrial Cardiomyopathy With Electrical, Contractile, and Structural Components: Evidence From Genetically-Engineered Rats. J Am Heart Assoc. 2017 Oct 28;6(11). Sun M, Ding J, Li D, Yang G, Cheng Z, Zhu Q. NUDT21 regulates 3'-UTR length and microRNA-mediated gene silencing in hepatocellular carcinoma. Cancer Lett. 2017 Dec 1;410:158-168. Liu XM, Zhang YL, Ji SY, Zhao LW, Shang WN, Li D, Chen Z, Tong C, Fan HY. Mitochondrial Function Regulated by Mitoguardin-1/2 Is Crucial for Ovarian Endocrine Functions and Ovulation. Endocrinology. 2017 Nov 1;158(11):3988-3999 Liu Y, Fan C, Zhang Y, Yu X, Wu X, Zhang X, Zhao Q, Zhang H, Xie Q, Li M, Li X, Ding Q, Ying H, Li D, Zhang H. RIP1 kinase activity-dependent roles in embryonic development of Fadd-deficient mice. Cell Death Differ. 2017 Aug;24(8):1459-1469 Zhang X, Wang L, Liu M, Li D*. CRISPR/Cas9 system: a powerful technology for in vivo and ex vivo gene therapy. Sci China Life Sci. 2017 May;60(5):468-475 （invited review） Zhang L, Li L, Gao G, Wei G, Zheng Y, Wang C, Gao N, Zhao Y, Deng J, Chen H, Sun J, Li D*, Zhang X*, Liu M*. Elevation of GPRC5A expression in colorectal cancer promotes tumor progression through VNN-1 induced oxidative stress. Int J Cancer. 2017 Jun 15;140(12):2734-2747 （Cover story） Chen Y, Ma J, Lu W, Tian M, Thauvin M, Yuan C, Volovitch M, Wang Q, Holst J, Liu M, Vriz S, Ye S*, Wang L*, Li D*. Heritable expansion of the genetic code in mouse and zebrafish. Cell Res. 2017 Feb;27(2):294-297. Lu J, Shao Y, Qin X, Liu D, Chen A, Li D*, Liu M*, Wang X*.CRISPR knockout rat cytochrome P450 3A1/2 model for advancing drug metabolism and pharmacokinetics research.Sci Rep. 2017 Feb 20;7:42922.. Chen R, Zhang Q, Duan X, York P, Chen GD, Yin P, Zhu H, Xu M, Chen P, Wu Q, Li D, Samarut J, Xu G, Zhang P, Cao X, Li J, Wong J. The 5-hydroxymethylcytosine (5hmC) reader Uhrf2 is required for normal levels of 5hmC in mouse adult brain and spatial learning and memory.J Biol Chem. 2017 Jan 23. pii: jbc.M116.754580 Wei Y, Chen Y, Qiu Y, Zhao H, Liu G, Zhang Y, Meng Q, Wu G, Chen Y, Cai X, Wang H, Ying H, Zhou B, Liu M, Li D, Ding Q. Prevention of Muscle Wasting by CRISPR/Cas9-mediated Disruption of Myostatin In Vivo.Mol Ther. 2016 Nov;24(11):1889-1891. You P, Hu H, Chen Y, Zhao Y, Yang Y, Wang T, Xing R, Shao Y, Zhang W, Li D*, Chen H*, Liu M*. Effects of Melanocortin 3 and 4 Receptor Deficiency on Energy Homeostasis in Rats. Sci Rep. 2016 Oct 7;6:34938. Chen Y, Liu X, Zhang Y, Wang H, Ying H, Liu M, Li D, Lui KO, Ding Q. A Self-restricted CRISPR System to Reduce Off-target Effects. Mol Ther. 2016 Sep;24(9):1508-10. Zhang L, Shao Y, Li L, Tian F, Cen J, Chen X, Hu D, Zhou Y, Xie W, Zheng Y, Ji Y, Liu M, Li D*, Hui L*. Efficient liver repopulation of transplanted hepatocyte prevents cirrhosis in a rat model of hereditary tyrosinemia type I. Sci Rep. 2016 Aug 11;6:31460 Chen Y, Lu W, Gao N, Long Y, Shao Y, Liu MZ, Chen H, Ye S, Ma X, Liu M*, Li D*. Generation of obese rat model by transcription activator-like effector nucleases targeting the leptin receptor gene. Sci China Life Sci. 2016 May 25.（IF，1.69） Luo J, Yang Z, Ma Y, Yue Z, Lin H, Qu G, Huang J, Dai W, Li C, Zheng C, Xu L, Chen H, Wang J, Li D, Siwko S, Penninger JM, Ning G, Xiao J, Liu M. LGR4 is a receptor for RANKL and negatively regulates osteoclast differentiation and bone resorption. Nat Med. 2016 May;22(5):539-46 Zhang Y, Zeng Z, Zhao J, Li D, Liu M, Wang X.Measurement of Rhodamine 123 in Three-Dimensional Organoids: A Novel Model for P-Glycoprotein Inhibitor Screening.Basic Clin Pharmacol Toxicol. 2016 Apr 6. Guan Y, Ma L, Li Q, Sun Z, Ma Li, Wu L, Wang L, Zeng L, Shao Y, Chen Y, Ma N, Lu W, Hu K, Han H, Yu Y, Huang Y, Liu M*, Li D*. CRISPR/Cas9 mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse. EMBO MOL MED. 2016 May 2;8(5):477-88.