个人简历

EDUCATION and TRAINING
1999-2003: Dalian University of Technology, China, B.S., Polymer
2003-2009: Dalian University of Technology, China, Ph. D., Biochemical engineering
2009-2011: Dalian University of Technology, China, Postdoctoral fellow, Biomedical engineering
Current grant support:
1. Fundamental Research Funds for the Central Universities (No.DUT19LAB17, 2019.01-2020.12)
2. Dalian Major Research and Development Program (2018.01-2020.12)
3. Dalian High Level Talent Innovation Support Project (No.2016RQ023, 2016.10-2019.09)
4. National Major Research and Development Program (No.2016YFC1103000, 2016.06-2020.12)
Book chapters:
1. Jun Ren, Houliang Wei, Li Xu, Lingyun Jia. Blood Detoxication. Comprehensive Biotechnology (3rd edition), Elsevier, 2019, 5, 723-734.
2. Jun Ren, Houliang Wei, Lingyun Jia. Adsorbents for the treatment of autoimmune diseases through hemoperfusion. Hemoperfusion: general and biospecific adsorbents, immunosorbents and leucocyte adsorbents, World Scientific, 2017, chapter 22: 629-648.
3. Jun Ren, Houliang Wei, Lingyun Jia. Blood Detoxication. Comprehensive Biotechnology (2nd edition), Elsevier, 2011, 5, 729-739.
Overview of my current research work
In general, I am very interested in understanding the mechanism of biomolecule recognition, which plays central role in a variety of life activities, and hope to exploit its potential application in different area of biotechnology and biomedical applications.
(1) Biomedical materials for blood purification treatment
One specific interest is to develop techniques that enable recognizing and separating some specific disease-related molecules directly from human blood circulation, for achieving blood purification treatment for a range of serious diseases. To this end, we have developed several strategies to design functional materials, including affinity ligand screening, camelid-derived single chain antibody or nanobody, and molecular imprinting et al. These techniques are used to synthesize selective adsorbents for removing pathogenic factors from the blood of patients suffering from autoimmune diseases, renal failure, sepsis, and even cancer. For example, we are using nanobody to develop blood purification materials to treat dialysis related amyloidosis (DRA) by selectively removing beta 2 microglobulin. Meanwhile we are also isolating nanobodies against cytokines for synthesizing selective adsorbents, which could be used to control “Cytokine Storm” by directly reducing circulating cytokines from the blood of critically-ill patients with deadly inflammation.
Currently, two of my patented blood purification adsorbents have been industrialized to develop medical appliances for treating autoimmune disease and DRA, respectively. The former has been in the stage of clinical trial in China.
(2) Biomolecular engineering for bioprocessing and biohybrid materials
Other studies focus on engineering functional proteins and developing approaches for bioseparation, biosensing, and biofunctional device design, which are mainly based on the construction of biohybrid material system. Current work is centered at the application of engineering nanobody. The development of the methodologies involves production of engineered nanobody, specific tagging, multivalued complex, site-specific modification, bioconjugation, surface modification, as well as addressing issues such as stability, efficiency and activity rate in the process of applications.
We have been developing chemical and biological methods that could enable the immobilization of nanobody or other functional proteins in a controllable and efficient manner, to facilitate the preparation of affinity chromatography media, and biosensor surfaces. We used photochemical crosslinking of phenol groups to immobilize proteins and prepare all-protein nanoparticles. The reactions could be completed within seconds without loss of protein activity. Nanobody-based multiprotein complexes have been designed to form multifunctional protein assemblies.
These technologies and methods are expected to be extended to other biomedical areas, such as assembly of targeted nanoparticles for drug delivery, disease detection and mechanistic studies.

教育经历
1996.9 1999.7 湖北省襄樊市第五中学 无
2003.9 2009.6 大连理工大学 生物化工 博士
1999.9 2003.7 大连理工大学 高分子专业 学士
工作经历
2011.9 至今 大连理工大学 讲师
2009.7 2011.8 大连理工大学 博士后
专利
一种层析介质及其制备方法和应用
利用多酚类化合物在材料表面实现血小板图案化的方法
一种层析介质及其制备方法和应用
一种重组蛋白A亲和配基的定点固定化方法
一种光子集成阵列免标记光学生化传感检测系统
一种重组人IgE受体蛋白的制备方法及应用
著作成果
Comprehensive Biotechnology, 2nd edition ，Volume 5: Medical Biotechnology and Healthcare/ Artificial Organs and Life Support Devices/ Blood Detoxication
Regenerative Medicine, Artificial Cells and Nanomedicine: Volume 4
科研项目
2016年大连科技之星项目(2015年称号)-用于清除肾衰中分子毒素的高效血液净化吸附剂, 省、市、自治区科技项目, 2016/10/11, 进行
利用原位点击化学构建蛋白质印迹识别界面, 2012/09/25, 完成
治疗肝衰竭肾衰竭血液净化新技术及新产品, 省、市、自治区科技项目, 2018/01/01, 进行
高性能吸附剂关键技术研发及产业化, 国家科技部 , 2016/07/28, 进行
仿生蛋白A血液净化材料及其制备方法, 企事业单位委托科技项目, 2014/12/05-2034/12/05, 进行
其他奖励
校第七届“硕士生学术之星”优秀指导教师奖 （2016年）
第十二届“挑战杯”辽宁省赛优秀指导教师 （2015年）

研究领域

1. Bioseparation techniques for protein purification.
2. Biomedical materials for blood purification treatments.""

近期论文

[1]Peng, Qiang,Zang, Berlin,Zhao, Wei,Li, Da,Ren, Jun,Ji, Fangling,Jia, Lingyun.Efficient continuous-flow aldehyde tag conversion using immobilized formylglycine generating enzyme[J],CATALYSIS SCIENCE & TECHNOLOGY,2020,10(2):484-492
[2]Han X.,Wang Z.,Shao Y.,Wu Z.,Ren J.,Wang J.,Teng J.,Zhao M..Temperature insensitive optical biosensor based on polymer waveguide microring[J],Guangxue Xuebao/Acta Optica Sinica,2015,35(11)
[3]Huang, Chundong,Li, Da,Ren, Jun,Ji, Fangling,Jia, Lingyun.Generation and Application of Fluorescent Anti-Human beta 2-Microglobulin VHHs via Amino Modification[J],MOLECULES,2019,24(14)
[4]暴晓博,任军,王玉凤,贾凌云.基于凝胶过滤色谱的β2微球蛋白标准品单体定量检测方法[J],色谱,2019,37(5):533-538
[5]Zang, Berlin,Ren, Jun,Li, Da,Huang, Chundong,Ma, Ha,Peng, Qiang,Ji, Fangling,Han, Lulu,Jia, Lingyun.Freezing-assisted synthesis of covalent C-C inked bivalent and bispecific nanobodies[J],ORGANIC & BIOMOLECULAR CHEMISTRY,2019,17(2):257-263
[6]Han, Xiu-You,Zhang, Hua,Bo, Shu-Hui,Morthier, Geert,Zhao, Ming-Shan,Wu, Zhen-Lin,Yang, Si-Cheng,Shen, Fang-Fang,Liang, Yu-Xin,Wang, Ling-Hua,Wang, Jin-Yan,Ren, Jun,Jia, Ling-Yun.Recent Progress of Imprinted Polymer Photonic Waveguide Devices and Applications[J],POLYMERS,2018,10(6)
[7]Zang, Berlin,Jia, Lingyun,Ren, Jun,Peng, Qiang.Facile preparation of covalent C-to-C linked nanobody fusions by freezing[J],ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY,2018,255
[8]贾凌云,任军.Facile preparation of covalent C-to-C linked nanobody fusions by freezing[A],2018,255:437-437
[9]臧柏林,贾凌云,任军,徐丽.冷冻法制备C端-C端连接的二价纳米抗体[A],中国化学会2017全国高分子学术论文报告会,2017,1
[10]任军,贾凌云,田凯凯.光催化交联法构建蛋白质水凝胶[A],中国化学会2017全国高分子学术论文报告会,2017,2
[11]Gao, Ming,Ren, Jun,Tian, Kaikai,Jia, Lingyun.Characterization of non-specific protein adsorption induced by triazole groups on the chromatography media using Cu (I)-catalyzed alkyne-azide cycloaddition reaction for ligand immobilization[J],JOURNAL OF CHROMATOGRAPHY A,2016,1476:63-68
[12]Ren, Jun,Tian, Kaikai,Jia, Lingyun,Han, Xiuyou,Zhao, Mingshan.Rapid Covalent Immobilization of Proteins by Phenol-Based Photochemical Cross-Linking[J],BIOCONJUGATE CHEMISTRY,2016,27(10):2266-2270
[13]贾凌云,任军,臧柏林,暴晓博.亲和分离材料的设计策略及其应用[A],2016,1-1
[14]任军,姚鹏,贾凌云.基于超分子置换洗脱模式的非盐依赖疏水色谱方法[A],2016,1-1
[15]臧柏林,任军,徐丽,贾凌云.一种重组蛋白A的定点固定化方法[A],2016,1-1
[16]Han, Lulu,Chu, Simin,Wei, Houliang,Ren, Jun,Xu, Li,Jia, Lingyun.Functionalized Magnetic Fe3O4-beta-Cyclodextran Nanoparticles for Efficient Removal of Bilirubin[J],JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2016,16(6):5537-5545
[17]贾凌云,任军,徐丽.Direct site-specific immobilization of protein A viaaldehyde-hydrazide conjugation[J],Journal of Chromatography B,2016,1008:132-138
[18]Zang, Berlin,Ren, Jun,Xu, Li,Jia, Lingyun.Direct site-specific immobilization of protein A via aldehyde-hydrazide conjugation[J],JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICALAND LIFE SCIENCES,2016,1008:132-138
[19]Han, Xiuyou,Shao, Yuchen,Han, Xiaonan,Lu, Zhili,Wu, Zhenlin,Teng, Jie,Ren, Jun,Zhao, Mingshan.Athermal optical waveguide microring biosensor with intensity interrogation[J],OPTICS COMMUNICATIONS,2015,356:41-48
[20]Yang, Liwei,Han, Lulu,Ren, Jun,Wei, Houliang,Jia, Lingyun.Coating process and stability of metal-polyphenol film[J],COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2015,484:197-205
[21]徐丽,王从纲,陈林丽,任军,谢健,贾凌云.通过分子簇获得不同聚集度的Aβ寡聚体及相互作用蛋白研究[A],2015全国老年痴呆与认知障碍相关疾病学术大会,2015,111-111
[22]Wei, Houliang,Han, Lulu,Tang, Yongchao,Ren, Jun,Zhao, Zongbin,Jia, Lingyun.Highly flexible heparin-modified chitosan/graphene oxide hybrid hydrogel as a super bilirubin adsorbent with excellent hemocompatibility[J],JOURNAL OF MATERIALS CHEMISTRY B,2015,3(8):1646-1654
[23]韩秀友,王凌华,任军,武震林,滕婕,谷一英,王锦艳,赵明山.聚合物光子集成器件及应用研究[A],第十一届全国塑料光纤.聚合物光子学学术会议论文集中国光学学会,2015,6-18
[24]韩秀友,韩笑男,王志灵,邵宇辰,武震林,任军,王锦艳,滕婕,赵明山.温度不敏感聚合物波导微环光学生化传感器研究[J],光学学报,2015,35(11):1113004
[25]Ren, Jun,Yao, Peng,Chen, Jingjing,Jia, Lingyun.Salt-independent hydrophobic displacement chromatography for antibody purification using cyclodextrin as supermolecular displacer[J],JOURNAL OF CHROMATOGRAPHY A,2014,1369:98-104
[26]Xu, Li,Wang, Conggang,Chen, Linli,Ren, Jun,Xie, Jian,Jia, Lingyun.Detection of A beta-interacting proteins via a novel A beta-adsorbents that use immobilized regular comb polymer[J],JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICALAND LIFE SCIENCES,2014,971:94-98
[27]Ren, Jun,Yao, Peng,Cao, Yaming,Cao, Jian,Zhang, Lijun,Wang, Yuanqiang,Jia, Lingyun.Application of cyclodextrin-based eluents in hydrophobic charge-induction chromatography: Elution of antibody at neutral pH[J],JOURNAL OF CHROMATOGRAPHY A,2014,1352:62-68
[28]Ren, Jun,Han, Pingping,Wei, Houliang,Jia, Lingyun.Fouling-Resistant Behavior of Silver Nanoparticle-Modified Surfaces against the Bioadhesion of Microalgae[J],ACS APPLIED MATERIALS & INTERFACES,2014,6(6):3829-3838
[29]徐丽,任军,谢健,贾凌云.Detection of Aβ-interacting proteins via a novel Aβ-adsorbents that use immobilized regular comb polymer[J],J Chromatogr B Analyt Technol Biomed Life Sci,2014,971(1):94-98
[30]Wei, Houliang,Han, Lulu,Ren, Jun,Jia, Lingyun.Anticoagulant Surface Coating Using Composite Polysaccharides with Embedded Heparin-Releasing Mesoporous Silica[J],ACS APPLIED MATERIALS & INTERFACES,2013,5(23):12571-12578
[31]Wei, Houliang,Ren, Jun,Han, Bo,Xu, Li,Han, Lulu,Jia, Lingyun.Stability of polydopamine and poly(DOPA) melanin-like films on the surface of polymer membranes under strongly acidic and alkaline conditions[J],COLLOIDS AND SURFACES B-BIOINTERFACES,2013,110:22-28
[32]Ren, Jun,Wang, Linghua,Han, Xiuyou,Cheng, Jianfang,Lv, Huanlin,Wang, Jinyan,Jian, Xigao,Zhao, Mingshan,Jia, Lingyun.Organic Silicone Sol-Gel Polymer as a Noncovalent Carrier of Receptor Proteins for Label-Free Optical Biosensor Application[J],ACS APPLIED MATERIALS & INTERFACES,2013,5(2):386-394
[33]韩秀友,谷一英,武震林,任军,贾凌云,王锦艳,蹇锡高.聚合物基光波导器件研究进展[A],2013,1-2
[34]赵明山,韩秀友,谷一英,武震林,任军,贾凌云,王锦艳,蹇锡高.微纳米压印聚合物光波导器件[A],2013,63-66
[35]Wei, Houliang,Xu, Li,Ren, Jun,Jia, Lingyun.Adsorption of bilirubin to magnetic multi-walled carbon nanotubes as a potential application in bound solute dialysis[J],COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2012,405:38-44
[36]Wang, Linghua,Ren, Jun,Han, Xiuyou,Claes, Tom,Jian, Xigao,Bienstman, Peter,Baets, Roel,Zhao, Mingshan,Morthier, Geert.A Label-Free Optical Biosensor Built on a Low-Cost Polymer Platform[J],IEEE PHOTONICS JOURNAL,2012,4(3):920-930
[37]Wang, Linghua,Zhao, Mingshan,Han, Xiuyou,Gu, Yiying,Lv, Huanlin,Cheng, Jianfang,Teng, Jie,Ren, Jun,Wang, Jinyan,Jian, Xigao.Optical biosensors utilizing polymer-based athermal microring resonators[A],Conference on Biophotonics - Photonic Solutions for Better Health CareIII,2012,8427
[38]任军,韩秀友,王锦艳,蹇锡高,赵明山,贾凌云.Organic silicone sol-gel polymer as non-covalent carrier of receptor proteins for label-free optical biosensor application[J],ACS Applied Materials & Interfaces,2012,5(2):386-394
[39]Wang, Linghua,Zhao, Mingshan,Kodeck, Valerie,Van Vlierberghe, Sandra,Ren, Jun,Teng, Jie,Han, Xiuyou,Jian, Xigao,Baets, Roel,Morthier, Geert.A low cost photonic biosensor built on a polymer platform[A],2011 Asia Communications and Photonics Conference and Exhibition, ACP 2011,2011,8311
[40]任军,白鹰,郝兰,贾凌云.MEP吸附剂对重症肌无力模型大鼠的疗效研究[A],第十三届全国生物材料学术会议,2011,328-328
[41]Ren, Jun,Bai, Ying,Hao, Lan,Dong, Yan,Pi, Zhiqian,Jia, Lingyun.Amelioration of experimental autoimmune myasthenia gravis rats by blood purification treatment using 4-mercaptoethylpyridine-based adsorbent[J],JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2011,98A(4):589-595
[42]任军,贾凌云.Amelioration of Experimental Autoimmune Myasthenia Gravis Rats by Blood Purification Treatment using[J],Journal of Biomedical Materials Research A,2011,98(4):589-595
[43]Ren, Jun,Bai, Ying,Hao, Lan,Pi, Zhiqian,Xu, Li,Jia, Lingyun.Amelioration of Experimental Autoimmune Myasthenia Gravis Rats Using 4-Mercaptoethylpyridine-based Adsorbent[J],JOURNAL OF BIOTECHNOLOGY,2010,150:S446-S446
[44]孙嘉怡,任军,徐丽,贾凌云.猪肝中单胺氧化酶B的分离纯化[J],色谱,2010,28(9):872-876
[45]Ren, Jun,Pi, Zhiqian,Lin, Xue,Xu, Li,Xie, Jian,Jia, Lingyun.Effect of Structural Properties of Both Proteins and Stationary Phases to Adsorption Behavior of Proteins in Hydrophobic Interaction Chromatography[A],4th International Conference on Bioinformatics and BiomedicalEngineering (iCBBE),2010
[46]Ren, Jun,Pi, Zhiqian,Lin, Xue,Xu, Li,Xie, Jian,Jia, Lingyun,Ma, Yue,Wang, Yumei.An Adsorbent for Extracorporeal Elimination of Pathogenic Autoantibodies[A],4th International Conference on Bioinformatics and BiomedicalEngineering (iCBBE),2010
[47]王巍,徐文学,任军,谢健,贾凌云.交联琼脂糖凝胶表面修饰对其性状影响的研究[J],应用化工,2009,38(7):937-940,949
[48]王巍,徐文学,任军,谢健,贾凌云.交联琼脂糖凝胶表面修饰对其性状的影响研究[A],第十七届全国色谱学术报告会,2009,938-940
[49]Ren, Jun,Jia, Lingyun,Xu, Li,Lin, Xue,Pi, Zhiqian,Xie, Jian.Removal of autoantibodies by 4-mercaptoethylpyridine-based adsorbent[J],JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICALAND LIFE SCIENCES,2009,877(11-12):1200-1204
[50]王舵,张丹妮,任军,贾凌云.分子对接技术在色谱配基筛选中的应用与优化[J],计算机与应用化学,2008,25(5):541-544
[51]孙宇,贾凌云,任军.蛋白质相互作用的研究方法[J],分析化学,2007,35(5):760-766
[52]任军,贾凌云.亲和色谱仿生配基的筛选和设计[J],分析化学,2005,33(9):1345-1349