刘波
近期热点
资料介绍
个人简历
人简介现任生物医学工程学院院长;全国智能传感器创新联盟理事。长期从事生物医学工程相关交叉领域的基础与应用研究工作,有着生物学、医学及工程学教育背景及国外博士后留学经历。目前主要将生物物理学、医学、细胞生物学以及组织工程、基因工程等学科的原理与方法相互结合。曾建立血管体外器官培养系统,整合压力、血流、温度等多种生物探头以及电机阀门等元器件,实现动态检测及自动调整体外器官的培养状态;曾经在国际上率先提出了生物物理学领域一个新的研究方向,即机械应力脉动频率对细胞功能的影响。这一研究方向的深入发展,有望解决心血管疾病、运动医学、肿瘤医学、航天医学等领域的一些重要基础研究问题。该方向其中一篇论文被生物物理领域国际著名杂志Biophysical Journal录用,该杂志审稿人评价该文在细胞应变反应的机制研究方面具有里程碑意义;本团队将诺贝尔奖得主钱永健(Roger Y. Tsien)教授在荧光蛋白技术基础上发展起来的FRET技术融入生物医学工程领域,实现了在机械应力下的活细胞内蛋白活性实时FRET检测,并应用生物力学、分子生物学及图像分析处理等多学科综合交叉的方法,研发新型细胞内蛋白活性的荧光探针,开展了不同力学信号对血管壁细胞功能改变的信号转导机制研究,取得了一系列有科学价值的成果。已经主持国家自然科学基金项目4项(No.31670867,No.31000060,No.30570459,No.10972139),参与国家自然科学基金重点项目2项(No.10732070,No.10132020)及12项国家自然科学基金面上项目。主持与企业合作的研发项目2项,通过无创伤生物探测技术研发相应的生物芯片以检测膀胱癌。完成相关论文已发表在Nature系列杂志Communications Biology、Cell系列杂志iScience、Nature系列期刊Scientific Reports、Toxicological Sciences(毒理学Top)、Cardiovascular Research(骨科学领域Top)、英国皇家学会Journal of Royal Society Interface、Journal of Orthopaedic Research(骨科学领域Top)等杂志上。累计在国内外期刊已经发表文章80余篇,单篇最高他引约70余次,并多次在国内外学术会议上交流。并获得《光明日报》、《中国科学网》、《大连日报》等多家主流媒体专门报道。目前正致力于新的荧光蛋白FRET生物探针设计制备与应用方面,以及在微流控芯片上进行活细胞蛋白活性动态检测等领域的工作。目前已经申报国家发明专利7项,实用新型专利3项,其中一项发明专利已经转让给企业。长期招收硕士生、博士生以及博士后,要求:专业背景生物学、生物工程、医学、生物医学工程,具备较强的英语听说读写能力,具备较强动手能力。教育经历2005.4 - 2007.12 上海交通大学 生物医学工程 博士 1998.9 - 2001.6 第二军医大学 解剖与组织胚胎学 硕士 1991.9 - 1995.6 华东师范大学 生物学 学士 1988.9 - 1991.6 四川邻水中学 工作经历2012.4 - 至今 大连理工大学 教授 1995.7 - 2004.8 第二军医大学 助教,讲师 2004.9 - 2012.3 上海交通大学 助研,副研 2008.9 - 2010.8 美国伊利若以大学香槟分校 博士后 专利:1. 刘波,王贤萌. 一种利用PDMS图章精准控制单个细胞形状的方法。20190926受理,专利申请号201910921374X2. 刘波,金禹航.一种纸芯片通用通道结构及其制备方法。20190613受理,专利申请号201910509971.13. 刘波,韩亭亭,耿春阳. 一种多指标联合检测标志性蛋白纸芯片。20190118受理,专利申请号201910048051.43. 刘波,耿春阳. 一种用于细胞高效抓捕的新型微流控阵列。 20180725已受理,专利申请号20180823719.34. 刘波,邓莎. 一种检测活细胞内Paxillin蛋白力传递的荧光生物探针。201712 06已受理,专利申请号201711274845.X5. 刘波,李旺,于新磊,张树利,孙坚原. 一种基于FRET技术检测细胞膜表面张力的生物探针。2019.04.01授权,专利号 201610752206.96. 刘波,邵帅,谢飞. 一种检测细胞内RhoGDIa活性动态变化的FRET探针。20160316受理,2019.01.01授权,专利号201610150171.17. 刘波,刘晓光,于新磊.两种不同单细胞之间相互作用的微流控芯片装置,国家发明专利,2016.06授权,专利号ZL201410466404.X8. 刘波,李驰宇,李旺,耿春阳,辛嘉龙. 一种可以同时检测尿液中膀胱癌细胞、结石、血细胞和细菌的微流控芯片,2016.08授权,专利号ZL201510263042.9 授课信息 解剖生理学 /2019-2020 /春学期 /2.0学分 /2020730030 解剖生理学 /2019-2020 /春学期 /32课时 /2.0学分 /2020730030 人体解剖学 /2019-2020 /秋学期 /52课时 /3.0学分 /1020740030 人体解剖学 /2019-2020 /秋学期 /52课时 /3.0学分 /1020740030 解剖生理学 /2018-2019 /春学期 /32课时 /2.0学分 /2020730030 生理学 /2018-2019 /春学期 /48课时 /3.0学分 /1020740040研究领域
研究方向微流控临床检测芯片:基于微流控技术研发新型芯片,实现临床疾病的早期快速低成本检测。FRET生物探针设计与应用:基于荧光蛋白技术设计可以在活细胞检测蛋白活性的探针结构,在相应的DNA层面上通过亚克隆构建探针,然后转入活细胞自行表达出探针蛋白,在活细胞上验证探针功能。细胞力学生物学:研究各种力学状态下,细胞通过何种信号途径,将物理的力学信号转化为细胞内生物化学信号,并调控细胞极性变化与定向迁移。"研究领域 1、机械应力在活细胞内定向物理传递调控细胞功能 机械应力作用于细胞引起细胞功能改变的机制,绝大多数研究者认为是应力通过细胞膜上的离子通道、G蛋白偶联受体、整合素等途径直接转化为细胞内的化学信号进行调控。但这一观点却不能解释细胞在定向应力作用下的定向排列和迁移现象。本团队提出,机械应力在活细胞内的物理传递调控细胞定向迁移这一新的研究方向,即机械应力在细胞内部存在直接通过细胞骨架等成分进行物理传递,到达细胞远端应力集中的部位激活局部蛋白分子,从而调控细胞的极性变化。围绕这一研究方向,本团队将诺贝尔奖得主钱永健(Roger Y. Tsien)教授发明的荧光蛋白FRET技术融入该领域,通过活细胞荧光图像动态分析与处理,获得探针在细胞内的动态变化,如细胞内局部的蛋白活性变化与应力变化等等。其中自行设计制备活细胞动态检测蛋白活性的多个FRET探针,可在活细胞内动态观察检测蛋白的活性时空变化;并首次实现了活细胞的表面张力可视化检测,获光明日报、中国科学报、科学网、大连日报等多家主流媒体专门报道。此部分工作论文相继发表于Nature 系列杂志Communications Biology、Scientific Reports、Cell系列杂志iScience、生物物理领域Biophysical Journal、英国皇家学会Journal of Royal Society Interface心血管领域Cardiovascular Research、以及骨科学Journal of Orthopaedic Research等杂志上。申报专利3项。2、肿瘤早期检测微流控芯片 膀胱癌是泌尿系统最常见的恶性肿瘤,其发病率和致死率较高,目前临床的技术手段对于膀胱癌的诊断和检测有诸多缺点和不足,例如检测过程有创伤、成本高、特异性较低等,并且很难实现早期检测诊断。微流控芯片技术因其低消耗、集成化、高灵敏度等优势,逐渐成为未来临床检测载体的必然发展趋势。本团队在前期多年研究积累的基础上,以微流控芯片技术为依托,开发了一种新型的用于膀胱癌检测微流控芯片,针对膀胱癌细胞的生物力学、蛋白活性等特异性变化,采用病人尿液作为样品,结合生物力学细胞抓捕、FRET技术精确检测等手段,实现无创伤检测、大幅度降低检测成本,并能够实现早于临床病理诊断的早期检测,可用于家庭自检、医疗机构普查及医院精确诊断。申报相关专利10项,其中一项已经转让。"近期论文
2020年 1. Xue CD*, Sun ZP, Li YJ, Chen JF, Liu B, Qin KR*. Separation of micro and sub-micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis Electrophoresis, DOI 10.1002/elps.202000002, January 3, 2020 (SCI: WOS: , JCR Q2, IF2018: 2.754)2. Na JT, Xue CD*, Li YJ, Wang Y, Liu B, Qin KR*. Precise generation of dynamic biochemical signals by controlling the programmable pump in a Y-shaped microfluidic chip with a ‘chiristmas tree’ inlet. Electrophoresis, doi.org/10.1002/elps.201900400, January 3, 2020 (SCI: WOS:000506393700001, JCR Q2, IF2018: 2.754)3. Fei Xie, Shuai Shao, Baohong Zhang, Sha Deng, Aziz Ur Rehman Aziz, Xiaoling Liao, Bo Liu*. Differential phosphorylation regulates shear stress-induced polar activity of Rho-specific guanine nucleotide dissociation inhibitor α. Journal of Cellular Physiology. 2020, DOI: 10.1002/jcp.29594 Accepted4. Peng Wang+, Chunyang Geng+, Lujun Pan*, Bo Liu*. Carbon Nanocoil Based Flexible Tip for Live Cell Study of Mechanotransduction and Electro-physiological Characteristics. Journal of Materials Chemistry B, 2020, 8, 1405 - 1410 DOI: 10.1039/C9TB02564B 2019年1. Aziz ur Rehman Aziz, Xiaohui Yu, Qingyun Jiang, Youyi Zhao, Sha Deng, Kairong Qin, Hanqin Wang*, Bo Liu*. Doxorubicin-induced toxicity to 3D-cultured rat ovarian follicles on a microfluidic chip. Toxicology in Vitro. 2019, 62: 104677 2. Wei Juan, Liu Chong, Jiang Yang, Duan Chunzheng, Chen Li, Li Wang, Liu Bo*, Li Jingmin*, Microfluidic device for generating regionalized concentration gradients under a stable and uniform fluid microenvironment. Journal of Micromechanics & Microengineering. 2019;29(1): 0150083. Baohong Zhang, Fei Xie, Aziz ur Rehman Aziz , Shuai Shao, Wang Li, Sha Deng, Xiaoling Liao, Bo Liu*.Heat Shock Protein 27 Phosphorylation Regulates Tumor Cell Migration under Shear Stress. Biomolecules. 2019, 9, 50; doi:10.3390/biom90200504. Aziz ur Rehman Aziz, Chunyang Geng, Wang Li, Xiaohui Yu, Kai-Rong Qin, Hanqin Wang*, Bo Liu*. Doxorubicin induces ER calcium release via Src in rat ovarian follicles. Toxicological Sciences. 2019, 168(1):171-178. doi: 10.1093/toxsci/kfy284 (毒理学领域Top杂志)5. Chen ZZ, Yuan WM, Xiang C, Zeng DP, Liu B, Qin KR. A microfluidic device with spatiotemporal wall shear stress and ATP signals to investigate the intracellular calcium dynamics in vascular endothelial cells. Biomechanics and Modeling in Mechanobiology, 2019, 18(1): 189-202 doi: 10.1007/s10237-018-1076-x EI:201837058001656. Wang, Yan-Xia, Liu, Hai-Bin, Li, Peng-Song, Yuan, Wen-Xue, Liu, Bo, Liu, Shu-Tian,Qin, Kai-Rong. ROS and NO Dynamics in Endothelial Cells Exposed to Exercise-Induced Wall Shear Stress[J], CELLULAR AND MOLECULAR BIOENGINEERING,2019,12(1):107-120 7. Yuan WM, Xiang C, Xue CD*, Liu B, Qin KR*. Measuring the apparent viscosities of single cells by tracking the entire deformation dynamics in microfluidic channels. Analytical Methods, 2019, 11, 5680-5690, Oct. 4, 2019 (SCI: WOS:000498560200003, JCR Q2, IF2018: 2.378, *Corresponding Author)8. Yuan WM, Shao JY, Xue CD *, Liu B, Qin KR*. A high-throughput microfluidic device for probing calcium dynamics of single cells squeezing through narrow channels. Journal of Micromechanics and Microengineering, 2019, 29(11):115014(9pp), Nov., 2019(SCI: WOS:000487125300002, JCR Q2/Q3, IF2018:2.141, *Corresponding Author) 2018年1. Shuai Shao†, Xiaoling Liao†, Fei Xie, Sha Deng, Xue Liu, Tapani Ristaniemi, Bo Liu*. FRET biosensor allows spatio-temporal observation of shear stress-induced polar RhoGDIα activation. Communications Biology, 2018, 1:224. DOI:10.1038/s42003-018-0232-2 (Nature子刊)2. Aziz ur Rehman Aziz, Sumbal Farid, Kairong Qin, Hanqin Wang*, Bo Liu*. Regulation of insulin resistance and glucose metabolism by interaction of PIM kinases and insulin receptor substrates. Archives of Physiology and Biochemistry. 2018. 1-10 DOI: 10.1080/13813455. 2018. 14989033. Fei Xie; Baohong Zhang; Wenfeng Xu; Xiaoling Liao*; Qiuhong Huang; Bo Liu*. Membrane fluidity regulates high shear stress-induced FAK activation at different subcellular compartments. Biocell. 2018, 41(2-3): 45-53(WOS:000450909600003)4. Wang Li, Xinlei Yu, Fei Xie, Baohong Zhang, Shuai Shao, Chunyang Geng, Aziz ur Rehman Aziz, Xiaoling Liao, Bo Liu*. A membrane-bound biosensor visualizes shear stress-induced inhomogeneous alteration of cell membrane tension. iScience, 2018, 7:180-190. DOI:10.1016/j.isci.2018.09.002 (Cell系列期刊)5. Geng C+, Li C+, Li W, Yan W, Li J, Aziz A U R, Zhai X, Sun W*, Liu B*. A Simple Fabricated Microfluidic Chip for Urine Sample-based Bladder Cancer Detection. Journal of Micromechanics & Microengineering, 2018, 28: 115011. doi: 10.1088/1361-6439/aae016(WOS:000446364100001)6. Wang Li, Chunyang Gen, Bo Liu*. Visualization of synaptic vesicle dynamics with fluorescence proteins. Folia Neuropathologica, 2018; 56 (1): 21-29. DOI: 10.5114/fn.2018.74656 (WOS:000428950700003)7. Wei Juan, Liu Chong, Jiang Yang, Liu Tao, Chen Li, Liu Bo*, Li Jingmin*. Uniform and stable supply of medium for in-vitro cell culture using a robust chamber. Journal of Micromechanics and Microengineering. 2018. 28(6) :065006· DOI: 10.1088/1361-6439/aab374 (WOS:000428847000001,EI:20181705110575)8. 耿春阳,李驰宇,李雅楠,任海军,翟晓峰,刘波*.一种捕获尿液中膀胱癌细胞的新型微流控芯片。中国医疗设备. 2018, 33(4):17-219. Aziz Ur Rehman Aziz, Sumbal farid, Kai-Rong Qin, Hanqin Wang*, Bo Liu*. PIM kinases and its relevance to the PI3K/AKT/mTOR pathway in regulation of ovarian cancer. Biomolecules. 2018, 8(1): 7; doi:10.3390/biom8010007 (WOS:000428550500007)10. Chiyu Li, Wang Li, Chunyang Geng, Haijun Ren, Xiaohui Yu, Bo Liu*. Microfludic chip for cancer cell detection and diagnosis. Journal of Mechanics in Medicine and Biology. 2018, 18(1): 1830001. DOI: 10.1142/S0219519418300016(WOS:000425310300004) 2017年1. Shuai Shao, Cheng Xiang, Kairong Qin, Aziz ur Rehman Aziz, Xiaoling Liao, Bo Liu*. Visualizing the spatiotemporal map of Rac activation in bovine aortic endothelial cells under laminar and disturbed flows. PLOS ONE. 2017 Nov 30;12(11):e0189088. doi: 10.1371/journal.pone.0189088. ( WOS:000416841900199)2. Aziz Ur Rehman Aziz†, Mengjie Fu†, Jiu Deng, Chunyang Geng, Yong Luo, Bingcheng Lin, Xiaohui Yu*, and Bo Liu*, A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle. Micromachines. 2017, 8(11), 335; doi:10.3390/mi8110335(WOS:000416801300021, EI: 20174904504625)3. Jingmin Li, Juan Wei, Yuanchang Liu, Bo Liu, Tao Liu, Yang Jiang, Laiqian Ding and Chong Liu. A microfluidic design to provide a stable and uniform in vitro microenvironment for cell culture inspired by the redundancy characteristic of leaf areoles. Lab on a Chip. 2017, 17, 3921–3933(WOS:000414805100017)4. Aziz Ur Rehman Aziz, Chunyang Geng, Mengjie Fu , Xiaohui Yu, Kairong Qin and Bo Liu*. The Role of Microfluidics for Organ on Chip Simulations. Bioengineering. 2017, 4(2), 39; doi:10.3390/ bioengineering4020039. (INSPEC:17068256)5. Fei Xie, Shuai Shao, Aziz ur Rehman Aziz, Baohong Zhang, Hanqin Wang, Bo Liu*. Role of Rho-specific guanine nucleotide dissociation inhibitor α regulation in cell migration. Acta Histochemica. 2017, 119 (3) :183 (WOS:000401049000001)6. 张宝宏,谢 飞,任海军,刘 波* . HSP27对细胞迁移的调控. 生物化学与生物物理进展. 2017,44(2):110-116 (WOS:000395049300002)7. Zongzheng CHEN, Weimo YUAN, A. R. AZIZ, Zhengming GAO, Depei ZENG, Bo LIU, Kairong QIN. Transfer characteristics of dynamic biochemical signals in non-reversing pulsatile flows in a shallow Y-shaped microfluidic channel: signal filtering and nonlinear amplitude-frequency modulation. Appl. Math. Mech. -Engl. Ed., 2017, 38(10):1481–1496 DOI 10.1007/s10483-017-2251-6 2016年1. Chen ZZ, Gao ZM, Zeng DP, Liu B, Luan Y, Qin KR. A Y-shaped microfluidic device to study the combined effect of wall shear stress and ATP signals on intracellular calcium dynamics in vascular endothelial cells, Micromachines, 2016, 7(11), 213, doi:10.3390/mi7110213 (WOS:000389131500018)2. Yu M, Chen ZZ, Xiang C, Liu B, Xie HD, Qin KR. Microfluidic-based single cell trapping using a combination of stagnation point flow and physical barrier, Acta Mechanica Sinica, 2016, 32(3), 422-429, doi:10.1007/s10409-016-0558-2 (WOS:000379181600007 EI)3. Wenfeng Xu#, Bo Liu#, Xue Liu, Jie Xu, Bo Li, Xiaoling Liao* .Regulation of BMP2-induced intracellular calcium increases in osteoblasts. Journal of Orthopaedic Research. 2016. 34(10):1725-1733. JOR-15-0392.R2 (23196) (WOS:000385745500007)(骨科学Top杂志)4. Wang YX, Xiang C, Liu B, Zhu Y, Luan Y, Liu ST, Qin KR. A multi-component parallel-plate flow chamber system for studying the effect of exercise-induced wall shear stress on endothelial cells. Biomed Eng Online. 2016. 28;15(Suppl 2):154. doi: 10.1186/s12938-016-0273-z. WOS:0003924247000225. Han Y, Wang L, Yao QP, Zhang P, Liu B, Wang GL, Shen BR, Cheng B, Wang Y, Jiang ZL, Qi YX. Nuclear envelope proteins Nesprin2 and LaminA regulate proliferation and apoptosis of vascular endothelial cells in response to shear stress. Biochim Biophys Acta. 2015;1853(5):1165-73. doi: 10.1016/j.bbamcr. 2015.02.013. 2015年之前代表性论文1. Liu, B. Shaoying Lu, Ying-li Hu, Xiaoling Liao, Mingxing Ouyang, Yingxiao Wang. RhoA and Membrane Fluidity Mediates the Spatially Polarized Src/FAK Activation in Response to Shear Stress. Scientific Reports. 2014;4:7008; DOI:10.1038/srep07008 (WOS:000344760500004) 2. Wenfeng Xu, Xiaoling Liao, Ling Zhang, and Bo Liu*. Tissue Induction, the Relationship between Biomaterial’s Microenvironment and Mesenchymal Stem Cell Differentiation. Journal of Biomedical Science and Engineering, 2013;6(1):85-913. Bo Liu, Shaoying Lu, Zonglai Jiang, Yingxiao Wang*. Two distinct phases of calcium signaling under flow. Cardiovascular Research, 2011, 91(1):124-133. (WOS:000291547500018) 4. Bo Liu, Tae-Jin Kim, Yingxiao Wang. Live cell imaging of mechanotransduction. Journal of The Royal Society Interface, 2010, 7(S3):S365-375 (WOS:000280131400008 EI:20104013276493)5. Liu B, Qu MJ, Qin KR, Li H, Li ZK, Shen BR, Jiang ZL. Role of cyclic strain frequency in regulating the alignment of vascular smooth muscle cells in vitro. Biophysical Journal, 2008; 94(4):1497–1507. (WOS 000252597700036) 6. Qu MJ*, Liu B*, Qi YX, Jiang ZL. Role of Rac and Rho-GDI Alpha in the frequency-dependent expression of h1-calponin in vascular smooth muscle cells under cyclic mechanical strain. Annals of Biomedical Engineering, 2008; 36(9): 1481–1488. (WOS 000258190500005 EI 20083311462312) 相关热点
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