个人简历

张运海，男，理学博士，中共党员，安徽农业大学教授（三级）、博士生导师、动物科技学院副院长，主要从事动物繁殖与生物技术应用基础研究；地方畜禽遗传资源保护与生物育种安徽省重点实验室常务副主任，安徽省现代牛羊产业技术体系副首席专家兼功能室主任、“115”产业创新团队首席助理，安徽省学术与技术带头人，安徽青年科技奖获得者，兼任中国农业生物技术学会动物生物技术分会常务理事、中国畜牧兽医学会动物繁殖学分会理事、安徽省细胞生物学学会理事、安徽省畜牧兽医学会理事；国家自然科学基金委网评专家、教育部科技奖网评专家；《Theriogenology》、《StemCellandDevelopment》、《JournalofAnimalScience》、《ReproductionFertilityDevelopment》、《AnimalGenetics》、《ReproductioninDomesticAnimals》、《遗传》、《中国细胞生物学学报》等期刊审稿人；主持或参与完成国家自然科学基金、转基因专项等国家级和省部级以上科研项目20余项；主持在研省级以上各类科研项目3项；我国首例体细胞克隆猪、国际首例手工克隆猪主创人之一。近年来，公开发表第一或通讯作者论文40余篇，其中SCI论文30多篇，H指数20；取得授权国家发明专利10余项。获国家科技进步奖二等奖1项、“神农中华农业科技奖”一等奖1项、安徽省科技一等奖1项、安徽省科技三等奖2项、安徽省教学成果二等奖1项；参与取得省级科技成果10多项。主持或参与完成教研项目3项，主持在研校级教研项目1项，参与国家级教研项目3项；指导校级大学生创新基金课题20余项（国家级3项，省级2项）。自2007年以来，指导25人获硕士学位，协助指导18人取得硕士学位；协助培养博士4人，培养毕业博士2人；目前指导研究生在读14人（硕士生9人、博士生5人）。

研究领域

动物繁殖与生物技术

近期论文

2019年
（1）Cao,Z#.,Xu,T.#,Tong,X.#,Zhang,D.,Liu,C.,Wang,Y.,Gao,D.,Luo,L.,Zhang,L.,Li,Y.,Zhang,Y.*,（2019）HASPINkinasemediateshistonedeacetylationtoregulateoocytemeioticmaturationinpigs.Reproduction(Cambridge,England).（SCI影响因子=3.086，JCR一区）
（2）Cao,Z#.,Luo,L#.,Yang,J.,Zhang,L.,Gao,D.,Xu,T.,Tong,X.,Zhang,D.,Wang,Y.,Li,Y.,Fang,F.,Zhang,Y.*,（2019）StimulatoryeffectsofNESFATIN-1onmeioticanddevelopmentalcompetenceofporcineoocytes.Journalofcellularphysiology.（SCI影响因子=3.923，JCR一区）
（3）Cao,Z.#,Zhang,M.#,Xu,T.,Chen,Z.,Tong,X.,Zhang,D.,Wang,Y.,Zhang,L.,Gao,D.,Luo,L.,Khan,I.M.,Zhang,Y.*,（2019）VitrificationofmurinematuremetaphaseIIoocytesperturbsDNAmethylationreprogrammingduringpreimplantationembryodevelopment.Cryobiology.（SCI影响因子=2.05，JCR二区）
（4）Chen,J.#,Pan,K.#,Chen,Z.,Li,Y.,Ding,B.,Han,C.,Cao,Z.,Bao,W.*,Zhang,Y.*,（2019）ProductionofporcineaminopeptidaseN(pAPN)site-specificeditedpigs.Animalsciencejournal90,366-371.（SCI影响因子=1.402，JCR二区）
（5）CaoZ#,XuT#,TongX,WangY,ZhangD,GaoD,ZhangL,NingW,QiX,MaY,YuT,KnottJG,ZhangY*(2019)Maternalyes-associatedproteinparticipatesinporcineblastocystdevelopmentviamodulationoftrophectodermepitheliumbarrierfunction.Cells,2019,8:1606-1622.(影响因子：5.5，中科院2区)
（6）CaoZ#,GaoD#,XuT,ZhangL,TongX,ZhangD,WangY,NingW,QiX,MaY,JiK,YuT,LiY,ZhangY*(2019)CircularRNAprofilingintheoocyteandcumuluscellsrevealsthatcircARMC4isessentialforporcineoocytematuration.Aging(AlbanyNY)11:8015-8034.(影响因子：5.6，中科院2区)
（7）LiY#,GaoD,XuT,AdurMK,ZhangL,LuoL,ZhuT,TongX,ZhangD,WangY,NingW,QiX,CaoZ,ZhangY*(2020)Anti-Mullerianhormoneinhibitsluteinizinghormone-inducedandrostenedionesynthesisinporcinethecacells.Theriogenology142:421-432.(影响因子：2.3，中科院2区).
（8）CaoZ#,ZhangD#,TongX,WangY,QiX,NingW,XuT,GaoD,ZhangL,MaY,YuT,ZhangY*(2020)Cumuluscell-derivedandmaternalSIRT6differentiallyregulatesporcineoocytemeioticmaturation.Theriogenology142:158-168.(影响因子：2.3，中科院2区)
（9）LuoL#,WangS,ZhuL,FanB,LiuT,WangL,ZhaoP,DangY,SunP,ChenJ,ZhangY,ChangX,YuZ,WangH,GuoR,LiB*,ZhangK*(2019)AminopeptidaseN-nullneonatalpigletsareprotectedfromtransmissiblegastroenteritisvirusbutnotporcineepidemicdiarrheavirus.ScientificReports9.
（10）LingY-h#,ZhengQ,LiY-s,SuiM-h,WuH,ZhangY-h*,ChuM-x,MaY-h,FangF-g,XuL-n(2019)IdentificationoflncRNAsbyRNASequencingAnalysisDuringinVivoPre-ImplantationDevelopmentalTransformationintheGoat.FrontiersinGenetics10.
（11）LingY#,ZhengQ,SuiM,ZhuL,XuL,ZhangY,LiuY,FangF,ChuM,MaY,ZhangX(2019)ComprehensiveAnalysisofLncRNARevealstheTemporal-SpecificModuleofGoatSkeletalMuscleDevelopment.InternationalJournalofMolecularSciences20.
（12）CaoZ#,GaoD,TongX,XuT,ZhangD,WangY,LiuY,LiY,ZhangY,PuY*(2019)Melatoninimprovesdevelopmentalcompetenceofoocyte-granulosacellcomplexesfromporcinepreantralfollicles.Theriogenology133:149-158.(影响因子：2.3，中科院2区)

2018年
（1）Cao,Z.B.#,Gao,D.,Xu,T.T.,Tong,X.,Wang,Y.Q.,Li,Y.S.,Fang,F.G.,Ding,J.P.,Zhang,X.R.,Zhang,Y.H.*,2018.Humanexhaledaircanefficientlysupportinvitromaturationofporcineoocytesandsubsequentearlyembryonicdevelopment.Anim.Reprod.15,29-38.
（2）LiYS#,LiuX#,ChenZ,SongDD,YangJ,ZuoXY,CaoZB,LiuY*,ZhangYH*.（2018）EffectofFollistatinonPre-ImplantationalDevelopmentofPigParthenogeneticEmbryos.AnimalScienceJournal,89(2):316-327.
（3）LiX#,ZhangPF#,JiangSS,DingB,ZuoXY,LiYS,CaoZB,ZhangYH*.（2018）Agingadultporcinefibroblastscansupportnucleartransferandtranscriptionfactor-mediatedreprogramming.AnimalScienceJournal,89(2):289-297.
2017年
（1）CaoZB#,WuRH#,GaoD,XuTT,LuoL,LiYS,HanJY,ZhangYH*.(2017).MaternalhistoneacetyltransferaseKAT8isrequiredforporcinepreimplantationembryodevelopment.Oncotarget,,8(52):90250-90261.
（2）ChenZ.#,ZuoX.#,LiH.,HongRY.,DingB.,LiuCX.,GaoD.,ShangH.,CaoZB.,HuangWP.,ZhangXR.,ZhangYH*.(2017)Effectsofmelatoninonmaturation,histoneacetylation,autophagyofporcineoocytesandsubsequentembryonicdevelopment.Animalsciencejournal,DOI:10.1111/asj.12779
（3）DingB#,CaoZB#,HongRY,LiH,ZuoX,LuoL,LiYS,HuangWP,LiWY,ZhangK*,ZhangYH*.(2017)WDR5inporcinepreimplantationembryos:expression,regulationofepigeneticmodificationsandrequirementforearlydevelopment.BiologyofReproduction,doi:10.1093/biolre/iox020
（4）GaoXX.#,YeJ.,YangC.,ZhangKF.,LiXM.,LuoL.,DingJP.,LiYS.,CaoHG.,LingYH.,ZhangXR.,LiuY.,FangFG.*,ZhangYH.*.(2017)ScreeningandevaluatingoflongnoncodingRNAsinthepubertyofgoats.BmcGenomics18.doi：10.1186/s12864-017-3578-9
（5）CaoZB.#,HongRY.#,DingB.,ZuoXY.,LiH.,DingJP.,LiYS.,HuangWP.,ZhangYH.*(2017)TSAandBIX-01294InducedNormalDNAandHistoneMethylationandIncreasedProteinExpressioninPorcineSomaticCellNuclearTransferEmbryos.PlosOne12:e0169092.doi：10.1371/journal.pone.0169092
（6）WangZF#,LiuCX#,HongRY,YangJ,LiH,LiYS,CaoHG,ZhangYH,CaoZB*.(2017)Effectofhistonedeacetylaseinhibitorromidepsinontheinvitrogrowthoffoetalfibroblastcellsandearlydevelopmentofporcineclonedembryos,ItalianJournalofAnimalScience,DOI:10.1080/1828051X.2016.1277963
（7）TaoJ#,ZhangY#,ZuoXY,HongRY,LiH,LiuX,HuangWP,CaoZB*,ZhangYH.(2017)DOT1Linhibitorimprovesearlydevelopmentofporcinesomaticcellnucleartransferembryos.PLoSONE12(6):e0179436.https://doi.org/10.1371/journal.pone.0179436
2016年
（1）YaoQ,ChenL,LiangY,SuiL,GuoL,ZhouJ,FanK,JingJ,ZhangYH*,YaoB*.(2016)Blastomereremovalfromcleavage-stagemouseembryosaltersplacentalfunction,whichisassociatedwithplacentaloxidativestressandinflammation.ScientificReports,6:25023.
（2）LingYH#,GuoXF,ChenT,DingJP,MaYH,ChuMX,DiR,ZhangYH*,ZhangXR*.(2016)CharacterizationandanalysisofdifferentiallyexpressedmicroRNAsinhircineovariesduringthefollicularandlutealphases.AnimalReproductionScience,166:47-57
（3）YangC#,YeJ#,LiX,GaoXX,ZhangKF,LuoL,DingJP,ZhangYH,LiYS,CaoHG,LingYH,ZhangXR,LiuY,FangFG*.(2016)DNAMethylationPatternsintheHypothalamusofFemalePubertalGoats.PLoSONE11(10):e0165327.https://doi.org/10.1371/journal.pone.0165327
2015年
（1）CaoZB,LiYS,ChenZ,WangH,ZhangML,ZhouNR,WuRH,LingYH,FangFG,LiN*,ZhangYH*.(2015)Genome-WideDynamicProfilingofHistoneMethylationduringNuclearTransfer-MediatedPorcineSomaticCellReprogramming.PLoSOne,10(12):e0144897
（2）LiuY,ZhangY,JiangQ,RaoM,ShengZ,ZhangY,DuW,HaoH,ZhaoX,XuZ,LiuJ,ZhuH.(2015)IdentificationofValidHousekeepingGenesforReal-TimeQuantitativePCRAnalysisofCollapsedLungTissuesofNeonatalSomaticCellNuclearTransfer-DerivedCattle.CellReprogramming,17(5):360-367（co-firstauthor)
（3）HanF,LiX,SongD,JiangS,XuQ,ZhangY*.(2015)SCNTversusiPSCs:proteinsandsmallmoleculesinreprogramming.InternationalJournalofDevelopmentalBiology,59(4-6):179-86.
（4）WeiChao,LiXia,ZhangPengfei,ZhangYu,LiuTong,JiangShaoshuai,HanFei,ZhangYunhai*.(2015)CharacterizationofPorcinePartiallyReprogrammediPSCsfromAdipose-DerivedStemCells.Reproduction,149(5):485-96,doi:10.1530/REP-14-0410
2014年
（1）CaoZubing,WuRonghua,SongDandan,HanFei,LiXia,ZhangPengfei,ZhangXiaorong,ZhangYunhai*.(2014)FrozenStorageofPorcineZygoteMedium3forInVitroCultureofClonedPorcineEmbryos.TheJournalofAnimal&PlantSciences,24(5):1288-1292
（2）Wang,J.,Zhao,G*.,Zhang,P.,Wang,Z.,Zhang,Y.*,Gao,D.,Zhou,P.,Cao,Y.(2014)Measurementofthebiophysicalpropertiesofporcineadipose-derivedstemcellsbyamicroperfusionsystem.Cryobiology69(3),442-50
（3）ZhouN.,CaoZ.,WuR.,LiuX.,TaoJ.,ChenZ.,SongD.,HanF.,LiY.,FangF.,ZhangX.,ZhangY.*(2014)DynamicchangesofhistoneH3lysine27acetylationinpre-implantationalpigembryosderivedfromsomaticcellnucleartransfer.AnimReprodSci148(3-4),153-63
（4）LiX.,ZhangP.,WeiC.,ZhangY.*,(2014)Generationofpluripotentstemcellsviaproteintransduction.InternationalJournalofDevelopmentBiology,21-27.
（5）ZhangY.,WeiC.,ZhangP.,LiX.,LiuT.,PuY.,LiY.,CaoZ.,CaoH.,LiuY.,ZhangX*.*,ZhangY*,(2014)Efficientreprogrammingofnaive-likeinducedpluripotentstemcellsfromporcineadipose-derivedstemcellswithafeeder-independentandserum-freesystem.PLoSOne9(1),e85089
（6）LiYS,ZhangCB,GaoY,ZhangYL,SuiLC,ZhangXR,ZhangYH*,(2014)Effectofepigallocatechin-3-gallateontheinvitrodevelopmentalpotentialofporcineoocytesandembryosobtainedparthenogeneticallyandbysomaticcellnucleartransfer.ItalianJournalofAnimalScience.13:3116doi:10.4081/ijas.2014.3116.
（7）Wang,J.,Cao,H.,Xue,X.,Fan,C.,Fang,F.,Zhou,J.,Zhang,Y.*,Zhang,X.*,(2014)EffectofvitaminCongrowthofcaprinespermatogonialstemcellsinvitro.Theriogenology81(4),545-55
（8）CaoZ,ZhouN,ZhangY,ZhangY,WuR,LiY,LiQ,ZhangY*,LiN*,(2014)Dynamicreprogrammingof5-hydroxymethylcytosineduringearlyporcineembryogenesis.Theriogenology81,496-508
（9）李侠,李运生,张运海*，(2014)猪多能干细胞的研究进展.中国细胞生物学学报,02:249-257.
2013年
（1）Gui,T.,Liu,X.,Tao,J.,Chen,J.,Li,Y.,Zhang,M.,Wu,R.,Zhang,Y.,Peng,K.,Liu,Y.,andZhang,X*.,ZhangY*,(2013)Validationofarecombinanthumanbactericidal/permeability-increasingprotein(hBPI)expressionvectorusingmurinemammaryglandtumorcellsandtheearlydevelopmentofhBPItransgenicgoatembryos.AnimReprodSci143(1-4),48-56
（2）Zhang,Y.,Zhang,X.,Liu,X.,Li,Y.,Ding,J.,Zhang,X.*,Zhang,Y.*,(2013)ReferenceGeneScreeningforAnalyzingGeneExpressionAcrossGoatTissue.Asian-AustralasianJournalofAnimalSciences26(12),1665-1671
（3）WeiC,LiuX,TaoJ,WuR,ZhangP,BianY,LiY,FangF,ZhangY*,(2014)EffectsofvitaminConcharacteristicsretainingofinvitroculturedmouseadiposederivedstemcells.InVitroCellular&DevelopmentalBiology–Animal,50(1):75-86
（4）ZhangXD,ZhangYH,LingYH,LiuY,CaoHG,YinZJ,DingJP*,ZhangXR*,(2013)CharacterizationanddifferentialexpressionofmicroRNAsintheovariesofpregnantandnon-pregnantgoats(Caprahircus).BMCGenomics,14:157-167(Co-firstAuthor).
（5）SuS,FangF,LiuY,LiY,RenC,ZhangY*,ZhangX*,(2013)ThecompensatoryexpressionofreproductivehormonereceptorsinthethymusofthemaleratfollowingactiveimmunizationagainstGnRH.GeneralandComparativeEndocrinology,185:57-66.
（6）ChenJ,ZhangY,ZhangY,WeiC,LiuX,ZhouN,JiaQ,LiY,ZhangX*,ZhangY*,(2013)ConstructionofmultipleshRNAsexpressionvectorthatinhibitsFUT1geneexpressionandproductionofthetransgenicSCNTembryosinvitro.MolecularBiologyReports,40(3):2243-2252.
（7）曹鸿国,陈涛,殷慧群,孙雪萍,蒲勇,杨盼,张运海,刘亚,李运生,陶勇,章孝荣，(2013)慢病毒直接诱导形成猪iPS细胞无需限定因子.生物化学与生物物理进展，(01)：57-63.
（8）陈建文,刘星,范彩云,李运生,包文斌,吴圣龙,章孝荣,张运海*，(2013)猪β防御素-1基因乳腺特异性表达载体的构建.扬州大学学报(农业与生命科学版)，(01)：1-6.
（9）吴蓉花,刘星,王维,张运海*，(2013)提高猪胚胎发育能力和克隆效率的研究进展.畜牧与兽医，(04)：89-94.