个人简介

黄勃，博士，1969年出生，教授，博士生导师，安徽省微生物防治重点实验室主任。安徽省森林保护和微生物防治领域专家，第十届安徽青年科技奖获奖人、省政府特殊津贴获得者。《菌物学报》和《中国生物防治学报》编委，教育部高校自然保护与环境生态类专业教学指导委员会委员。 1997.9-2001.8在中国科学技术大学就读分子生物学专业博士研究生，从事虫生真菌的分子系统学和分子分类的研究，获博士学位。2005.1-2007.1为Cornell大学植物病理系访问学者，从事虫霉目的分子系统学的研究。1995年至今，在安徽农业大学森林保护学教研室任教，历任助教、讲师，副教授和教授。主持省级以上科技项目15项，其中国家级项目有8项，包括7项国家自然基金、1项林业行业公益性项目和1项教育部“新世纪优秀人才支持计划”。 曾获得安徽省自然科学一等奖（第三名，第六名）二项及省科技进步一等奖（第七名）、省科技进步二等奖（第六名）和省科技进步三等奖（第2名）各一项。目前在GenBank中注册了350多个虫生真菌DNA片断序列和10个功能基因的cDNA全序列，并运用分子生物学手段解决了一些虫生真菌长期存在的分类难题。发表真菌新属3个，新种15个，新组合23个。在全国分离鉴定了大量菌种，通过4套保藏系统妥善保藏了8000多株，是我国最大、名列世界前五位的虫生真菌保藏中心，为我国真菌杀虫剂生产企业提供了大量的优质生产菌株。近年来在世界上率先完成了绿僵菌基因组水平的miRNA、长链非编码RNA和DNA甲基化系统鉴定，确定和注释4个了绿僵菌非编码小RNA分子通路中的重要基因——2个Argonaute和2个Dicer的功能；从转录水平上揭示了孢子高温胁迫响应的分子机制。迄今已在国内外学术期刊发表菌物相关论文170多篇（SCI源期刊近70篇）。并被SCI论文引用在700次以上，其中他引超过550次。　主要教学经历与成果：主要承担本科生“林木病理学”、硕士研究生“昆虫真菌学”和博士研究生“森林微生物生态进展”教学

研究领域

虫生真菌分子生物学、分子系统学和害虫的生物防治。

主要科研项目：1、国家自然科学基金面上项目：MT-A70蛋白介导的RNA甲基化调控罗伯茨绿僵菌杀虫毒力的分子机理研究（31972332），59万，主持，2020.01-2023.122、国家自然科学基金面上项目：微小RNA(miR-7)靶向调控罗伯茨绿僵菌产孢的分子机理研究（31772226），60万，主持，2018.01-2021.123、国家自然科学基金面上项目：绿僵菌的致病相关小RNA对寄主靶基因调控研究（31471821），2015.1-2018.12，主持, 87万4、国家重点研发计划“活体生物农药增效及有害生物生态调控机制”项目子课题（2017YFD0200400）：绿僵菌的MicroRMA与寄主互作的机理研究， 70万，主持，2017.01-2020.12。5、国家自然科学基金面上项目：金龟子绿僵菌产孢调控中微小RNA及其靶基因的鉴定与功能研究（31272096）， 2013.1-2016.12，主持，75万，6、林业公益性行业科研专项：山核桃有害生物绿色防控技术研究与示范（201204506），2012.1-2016.12，主持，154万7、国家自然科学基金面上项目：球孢白僵菌的繁殖与自然群体交配型关系的研究（31070009），2011-2013，主持，30万8、国家自然科学基金面上项目：耳霉属分子系统学及其中国资源研究（30770008），2008-2010，主持，30万9、教育部“新世纪优秀人才计划”（ NCET-05-0560），教育部，2006-2008，主持，50万10、“安徽省优秀青年基金”滚动支持（08040106902），安徽省自然基金委，2008-2009，主持，10万11、安徽省“十一五”攻关：真菌杀虫剂系列化产品的研发及应用技术的研究（06013053A），2006-2008，主持，30万12、安徽省优秀青年基金（04041043），安徽省自然基金委，2004-2006，主持，10万13、国家自然科学基金青年项目：利用多位点序列分析研究白僵菌中的复合种（30300004），2004-2006，主持，17万　主要科研成果：

近期论文

代表性论文论著：1、Xie XY, Wang YL, Yu DS, Xie R, Liu ZB, Huang B（通讯作者）. 2020. DNM1, a dynamin-related protein that contributes to endocytosis and peroxisome fission, is required for the vegetative growth, sporulation, and virulence of Metarhizium robertsii. Appl Environ Microbiol 86:e01217-20. https://doi.org/10.1128/AEM.01217-20.2、Luo FF, He YQ, Wei J, Zhao C, Zhou X, Hu FL（通讯作者）, Lu Ruili, Bao Guanhu, Huang Bo（通讯作者）, 2020. Basidiosins A and B: Cyclopentapeptides from the entomophthoralean fungus Basidiobolus meristosporus. Fitoterapia. https://doi.org/10.1016/j.fitote.2020.1046713、Tong YM, Wu H, Liu ZB, Wang ZX, Huang B（通讯作者）， 2020. G-protein subunit Gai in mitochondria, MrGPA1, affects conidiation, stress resistance, and virulence of entomopathogenic fungus Metarhizium robertsii. Frontiers in Microbiology. DOI: 10.3389/fmicb.2020.012514、Wang YL , Nie Y, Yu DS, Xie XY, Qin L, Yang Y, Huang B（通讯作者）, 2020. Genome-wide study of saprotrophy-related genes in the basal fungus Conidiobolus heterosporus. Applied Microbiology and Biotechnology. DOI 10.1007/s00253-020-10698-65、Yang GG, Hu F, Shi NJ, Wang P, Huang B（通讯作者）, 2020. A novel non-segmented double-stranded RNA virus isolated from the basal fungus Conidiobolus sp. Archives of Virology, DOI: 10.1007/s00705-020-04692-66、Nie Y, Yu DS, Wang CF, Liu XY（通讯作者）, Huang B（通讯作者）， 2020. A taxonomic revision of the genus Conidiobolus (Ancylistaceae, Entomophthorales): four clades including three new genera. MycoKeys 66: 55–817、Wang P, Yang GG, Shi NJ, Huang B（通讯作者）, 2020. Molecular characterization of a new partitivirus, MbPV1, isolated from the entomopathogenic fungus Metarhizium brunneum in China. Archives of Virology 165:765-769. DOI 10.1007/s00705-019-04517-18、Wang YL , Wang ZX, Yang WJ, Xie XY, Cheng HY, Qin L, Tang GL（通讯作者） , Huang B（通讯作者）, 2019. The Degradation of fungal microRNAs triggered by Short Tandem Target Mimic (STTM) is via the small RNA degrading nuclease. Applied and Environmental Microbiology, 85 (9): e03132-189、Wang ZX, Jiang YY, Wu H, Xie XY, Huang B（通讯作者）, 2019. Genome-wide identification and functional prediction of long non-coding RNAs involved in the heat stress response in Metarhizium robertsii. Front. Microbiol. 10: 2336. doi: 10.3389/fmicb.2019.0233610、Shi NJ, Yang GG, Wang P, Wang YL, Yu DS, Huang B（通讯作者）, 2019. Complete genome sequence of a novel partitivirus from the entomogenous fungus Beauveria bassiana in China. Archives of Virology, 164（12）: 3141-3144 doi:10.1007/s0070 5-019-04428 -111、Wang ZX, Zhu H, Cheng YR, Jiang YY, Li YD, Huang B（通讯作者）, 2019. The polyubiquitin gene MrUBI4 is required for conidiation, conidial germination, and stress tolerance in the filamentous fungus Metarhizium robertsii. Genes, 10, 412; doi:10.3390/genes1006041212、Wang ZX, Jiang YY, Li YD, Feng JY, Huang B（通讯作者）, 2019. MrArk1, an actin-regulating kinase gene, is required for endocytosis and involved in sustaining conidiation capacity and virulence in Metarhizium robertsii. Applied Microbiology and Biotechnology, 103(12): 4859-4868; DOI : 10.1007/s00253-019-09836-613、He YQ, Zhang WC, Peng F, Lu RL, Zhou H, Bao GH, Wang B, Huang B（通讯作者）, Li ZZi, Hu FL（通讯作者）, 2019. Metabolomic variation in wild and cultured cordyceps and mycelia of Isaria cicadae. Biomedical Chromatography, 33(4): e447814、Xie T; Wang YL; Yu DS; Zhang QL; Zhang TT; Wang ZX; Huang B（通讯作者）, 2019. MrSVP, a secreted virulence-associated protein, contributes to thermotolerance and virulence of the entomopathogenic fungus Metarhizium robertsii, BMC Microbiology, 19(25)：1~1015、Nie Y, Wang L, Cai Y, Tao W, Zhang YJ（通讯作者）, Huang B（通讯作者）, 2019. Mitochondrial genome of the entomophthoroid fungus Conidiobolus heterosporus provides insights into evolution of basal fungi. Applied Microbiology and Biotechnology, 103: 1379–139116、Chen MJ , Huang B（通讯作者）, 2019. Conidiobolus antarcticus, a synonym of C. osmodes. Mycotaxon, 133: 635-64117、Zhou R, Zhou XZ, Fan AL, Wang ZX, Huang B（通讯作者）, 2018. Differential functions of two metalloproteases, Mrmep1 and Mrmep2, in growth, sporulation, cell wall integrity, and virulence in the filamentous fungus Metarhizium robertsii. Frontiers in Microbiology 9: e1528.18、Yang WJ, Wu H, Wang ZX, Sun Q, Qiao LT, Huang B（通讯作者）, 2018. The APSES gene MrStuA regulates sporulation in Metarhizium robertsii. Frontiers in Microbiology 9 e1208.19、Nie Y, Qin L, Yu DS, Liu XY, Huang B（通讯作者）, 2018. Two new species of Conidiobolus occurring in Anhui, China. Mycological Progress, 17(10): 1203-121120、Zhang YB, Wang YL, Li WH, Bao LN, Wang LH, Huang XH, Huang, B（通讯作者）, 2018. Biogas emission from an anaerobic reactor. Aerosol and Air Quality Research, 18(6): 1493-1502.21、Wang ZX, Zhou Q, Li YD, Qiao LT, Pang Q, and Huang B（通讯作者）, 2018. iTRAQ-based quantitative proteomic analysis of conidia and mycelium in the filamentous fungus Metarhizium robertsii. Fungal Biology, 122(7): 651-65822、Liu YJ, Huang B（通讯作者）, 2018. Pestalotiopsis mangiferae causes leaf spot of chinese hickory (Carya cathayensis) in China. Plant Disease 102 (3) : 674-674.23、Meng HM, Wang ZX , Wang YL, Zhu H, Huang B（通讯作者）, 2017. Dicer and argonaute genes involved in RNA interference in the entomopathogenic fungus Metarhizium robertsii. Applied and Environmental Microbiology 87（6）：e03230-16.24、Wang YL, Wang TT, Qiao LT, Fan JR, Zhang TT, Wang ZX, Li WZ,Chen AH, Huang B（通讯作者）, 2017. DNA methyltransferases contribute to the fungal development stress tolerance and virulence of the entomopathogenic fungus Metarhizium robertsii. Appl Microbiol Biotech 101（10）: 4215-4226.25、Li WZ, Wang YL, Zhu JY, Wang ZX, Tang GL, Huang B（通讯作者）, 2017. Differential DNA methylation may contribute to temporal and spatial regulation of gene expression and the development of mycelia and conidia in entomopathogenic fungus Metarhizium robertsii. Fungal Biology 21 ( 2 ): 293 -303.26、Nie Y, Tang XX, Liu XY, Huang Bo（通讯作者）, 2017. A new species of Conidiobolus with chlamydospores from Dabie Mountains, Eastern China. Mycosphere 8 (7): 809-816.27、Chen AH, Wang YL, Shao Y, Huang B（通讯作者）, 2017. A novel technique for rejuvenation of degenerated caterpillar medicinal mushroom, Cordyceps militaris (Ascomycetes), a valued traditional chinese medicine. International Journal of Medicinal Mushrooms 19 (1)：87-91.28、Zhang LP, Wang ZX, Wang YL, Huang B （通讯作者）, 2017. Transcriptomic profile of lignocellulose degradation from Trametes versicolor on poplar wood. BioResources 12 (2): 2507-2527.29、Nie Y, Tang XX, Liu XY, Huang B（通讯作者）, 2016. Conidiobolus stilbeus, a new species with mycelial strand and two types of primary conidiophores. Mycosphere7 (6), 801–808.30、Meng HM, Wang ZX , Meng XY , Xie L， Huang Bo（通讯作者）, 2015. Cloning and expression analysis of the chitinase gene Ifu-chit2 from Isaria fumosorosea. Genetics and Molecular Biology 38 ( 3 ): 381-38931、Wang YL, Wang ZX, Liu C, Wang SB（通讯作者）, Huang B（通讯作者）,2015. Genome-wide analysis of DNA methylation in the sexual stage of the insect pathogenic fungus Cordyceps militaris. Fungal Biology, 119(12) : 1246-125432、Wang ZX, Zhou XZ, Meng HM, Liu YJ, Zhou Q， Huang B（通讯作者）, 2014. Comparative transcriptomic analysis of the heat stress response in the filamentous fungus Metarhizium anisopliae using RNA-Seq. Applied Microbiology and Biotechnology, 98(12): 5589-559733、Liu Y J, Huang B (通讯作者), Wu ZH, 2014.First report of Pestalotiopsis vismiae causing trunk disease of Chinese hickory (Carya cathayensis) in China. Plant Disease, 98 (11): 1588-1588 34、Zhu H, Wang ZX, Luo XM, Song JX, Huang, B(通讯作者）, 2014. Effects of straw incorporation on Rhizoctonia solani inoculum in paddy soil and Rice Sheath blight severity. Journal of Agricultural Science, 152 （5）: 741-748 35、Shi CE, Wang C, Xu XN, Huang B (通讯作者), 2014. Comparison of bacterial communities in soil between nematode-infected and nematode-uninfected Pinus massoniana pinewood forest。 Applied Soil Ecology， 85 : 11-20 36、Xie L, Chen HM, Wang ZX, Huang B（通讯作者）, 2014. Isolation of cDNA and upstream sequence of a gene encoding trehalose-6-phosphate synthase 1 from Beauveria bassiana and its functional identification in Pichia pastoris. Biologia, 69(8): 959-967 37、Wang ZX , Meng HM , Zhuang ZL , Chen MJ , Xie L， Huang B（通讯作者）, 2013. Molecular cloning of a novel subtilisin-like protease (Pr1A) gene from the biocontrol fungus Isaria farinosa, Applied Entomology and Zoology, 48 ( 4 ): 477-487338、Cai Y, Pu SC, Nie Y, Rehner SA, Huang B（通讯作者）, 2013. Discrimination of Chinese Beauveria strains by DGGE genotyping and taxonomic identification by sequence analysis of the Bloc nuclear intergenic region. Applied Entomology and Zoology. 48(3): 255-26339、Chen MJ, Huang B（通讯作者）, Li ZZ, Spatafora W J, 2013. Morphological and genetic characterisation of Beauveria sinensis sp. nov. from China. Mycotaxon, 124, 301–308.40、Zhou Q, Wang ZX, Zhang J, Meng HM, Huang B（通讯作者）, 2012. Genome-wide identification and profiling of microRNA-like RNAs from Metarhizium anisopliae during development. Fungal Biology,116, 1156-1162．41、Nie Y, Yu CZ, Liu XY, Huang B（通讯作者）, 2012. A new species of Conidiobolus (Ancylistaceae) from Anhui, China. Mycotaxon. 120：427–435.42、Zhang SL, He LM, Chen X，Huang B（通讯作者）,2012. Beauveria lii sp. nov. isolated from Henosepilachna vigintioctopunctata. Mycotaxon. 121：199–206.43、Chen MJ, Zhou N, Li ZZ, Sung G-H, Huang B（通讯作者）, 2010. Paecilomyces echinosporus sp. nov., a species isolated from soil in China. Mycotaxon 114:25-32。44、Pu SC,Chen MJ，Ma ZY，Xie L，Li ZZ，Huang B（通讯作者），2010.Genotyping isolates of the entomopathogenic fungus Beauveria bassiana sensu lato by Multi-locus PCR-DGGE analysis. African Journal of Biotechnology, 9(27): 4290-4294．45、Huang B, Humber AR, Hodge TK, 2007. A new species of Conidiobolus from Great Smokies National Park. Mycotaxon 100: 227-233.46、Huang B, Li CR, Humber AR, Hodge TK, Fan MZ, Li ZZ, 2005. Molecular evidence for the taxonomic status of Metarhizium taii and its teleomorph, Cordyceps taii (Hypocreales, Clavicipitaceae). Mycotaxon 94：137-147.47、Huang B, Humber AR, Li SG, Li ZZ, Hodge TK, 2005. Further notes on the molecular taxonomy of Metarhizium. Mycotaxon 94: 181-187.48、Huang B, Li CR, Fan MZ， Li ZZ, 2002. Moleculer identification of the teleomorph of Beauveria bassiana. Mycotaxon 81：229-236．